assistive reproductive technologies

Question 1

when was copy cat(CC) cloned

Answer 1

2001

Question 2

who was CC’s genetic donor, what type of cell was used

Answer 2

rainbow, cumulus cells

Question 3

why are calico cats almost always female

Answer 3

bc it relies on an x-chromosome inactivation

Question 4

when was the first calf born from IVP

Answer 4

1981

Question 5

when was the first human born from IVP

Answer 5

1978

Question 6

steps for IVF

Answer 6

-aspirate eggs, transfer to uterus(4-8 cell stage)
-ovarian stimulation

Question 7

oocyte recovery methods for IVF

Answer 7

-surgery(old)
-ultrasound-guided transvaginal oocyte recover

Question 8

what is GIFT

Answer 8

oocyte + sperm tranferred into oviduct(in vivo fertilization)

Question 9

what is ZIFT

Answer 9

-zygote intrafallopian transfer
-transfer into oviduct at 1-cell stage(in vitro fertilization)

Question 10

what is blastocyst transfer

Answer 10

culture in vitro to day 5-6 then transfer to uterus

Question 11

intrauterine insemination

Answer 11

delivered into uterus

Question 12

ICSI

Answer 12

-sperm deposited directly into oocyte cytoplasm

Question 13

what conditions is ICSI recommended for

Answer 13

-limited sperm available
-only non-motile sperm available
-only testicular or epididymal sperm available

Question 14

IVP procedure

Answer 14

1. in vitro oocyte maturation
2. in vitro fertilization
   2.5. sex sorting, cloning, cryopreservation
3. culture in vitro
4. embryo transfer

Question 15

cloning procedure

Answer 15

1. remove DNA from metaphase II oocyte
2. remove cells from donor embryo/animal
3. fuse donor cell with enucleated oocyte
4. culture to blastocyst phase in lab then transfer to recipient

Question 16

first clone produced from somatic cells

Answer 16

Dolly(sheep)

Question 17

what is used for gene editing

Answer 17

CRISPR-Cas9

Question 18

T/F gene editing is considered as GMOs

Answer 18

FALSE