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Microbiology > Bacteriology- Nocardia, Actinomyces, Mycobacterium > Flashcards

Bacteriology- Nocardia, Actinomyces, Mycobacterium Flashcards

(44 cards)

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1
Q

Motility of listeria

A

Tumbling at 25 C
Non motile at 37 C

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2
Q

Listeria
1. Flagella
2. Hemolysis shown
3. Infection via?
4. Characteristics
5. Diagnosis

A
  1. Peritrichous flagella
  2. Beta
  3. Cold contaminated food, dairy. Causes diarrhoea
  4. Catalase +, Camp +, Cold enrichment at 4 C
  5. ELISA
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3
Q

Neonatal infection of Listeria is called

A

Granulomatosis infanti septicum

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4
Q

Treatment of Listeria infection

A

Ampicillin
Gentamicin

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5
Q

Virulence of M. tb

A

Cord factor

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6
Q

What is the reason behind acid fastness of M. tb

A

Mycolic acid

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7
Q

What is the function of LAM in cell wall of M. tb?

A

It inhibits phagolysosome formation.
LAM can be tested in urine in cases of HIV with TB

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8
Q

Ghon’s focus vs Ghon’s complex vs Ranke’s complex

A

Ghon’s focus– Lung lesion
Ghon’s complex– Lung + Lymph node
Ranke’s complex– calcified Ghon’s complex

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9
Q

Simon’s focus

A

Apex involvement of TB

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10
Q

Simmond’s focus

A

Liver involvement of TB

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11
Q

Assman’s focus

A

Infraclavicular involvement of TB

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12
Q

Puhl’s

A

Supraclavicular involvement of TB

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13
Q

Weighert’s focus

A

Blood vessel involvement of TB

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14
Q

Rich’s focus

A

Brain involvement of TB

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15
Q

M. TB vs M. bovis

A

M. Tb is catalase, Niacin, Nitrate reduction positive. M. bovis is negative for all three
M.tb colonies are rough, buff and tough. M. bovis colonies are smooth, white and breaks easily

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16
Q

Sputum sample of M. tb is taken via

A

Modified Petroff method with 4% NaOH or further modified to NALC with 2% NaOH

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17
Q

ZN stain sensitivity
Lowenstein Jensen Media sensitivity for AFB

A

ZN: 10^4 AFB/ml
LJ media: 10-100 AFB/ml

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18
Q

Composition of LJ media

Study These Flashcards
A

Malachite green
Egg
Salt
Asparagine

19
Q

Newer automated methods for TB detectiom

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A
  1. Bactec MGIT- Mycobacterium Growth Indicator Tube
    It is fluorescent based- uses middlebrook 7H9 broth
  2. BacT/ alert- colorimetry based
  3. Versatrek- detects pressure changes
20
Q

Molecular typing methods for TB:

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A
  1. RFLP using 1S6110 strain
  2. CBNAAT/ GeneXpert- within 2 hrs. Can detect 131 organism/ ml min
    It can also detect resistance (rpoB gene)
  3. Line probe assay
21
Q

Tests to detect latent TB

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A
  1. Mantoux: 0.1 ml PPD
  2. Interferon Gamma Release Assay
    IF gamma is needed for granuloma formation. If T lymphocyte sample is exposed to CFP10, TB7.7, ESAT 6–> releases IF gamma which is measured by ELISA
22
Q

False + Mantoux test
False - Mantoux test

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A
  1. BCG vaccine, non TB mycobacterium
  2. HIV, Immunodeficiency
23
Q

Classification for non TB mycobacteria

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A

Runyon’s classification
Photochromogens
Scotochromogens
Non photochromogens
Rapid growers

24
Q

Photochromogens Examples

Study These Flashcards
A

M. marinum
M. kansasii

25
Scotochromogens examples
M. scrofulaceum M. gordonae M. szulgai
26
Non photochromogens examples
M. avium M. xenopi M. ulcerans ‘AUX’
27
Rapid growers examples in Runyon classification
M. chelonae M. fortuitum They grow within 7 days
28
Which organism causes swimming pool granuloma/ fish tank granuloma?
M. marinum p/w warty skin lesions
29
Which are the tap water scotochromogens?
M. gordonae
30
Which atypical mycobacteria causes Buruli ulcer?
M. ulcerans
31
M. ulcerans produces an exotoxin called?
Mycolactone exotoxin
32
What constitutes MAC?
M. avium, M. intracellulare
33
Lady Windermere syndrome is caused by?
MAC
34
Which atypical mycobacterium causes post surgery abscess?
M. chelonae
35
Hansen's bacillus is
M. leprae
36
Features of M. leprae
Obligate anerobe Obligate intracellular- on foot pad of mice or armadillo
37
MC site of infection of nocardia vs actinomyces
Nocardia- lung. LN absent Actinomyces- jaw. LN present
38
MC extrapulmonary site of Nocardia infection
Brain
39
Molar tooth appearance on solid media is shown by
Actinomyces
40
Culture of Nocardia is done by
Paraffin bait technique
41
Tx of Nocardia
Sulfonamides
42
Tx of Actinomyces
Penicillin
43
Splendore Hoeppli phenomenon is seen in
Actinomyces Sporothrix fungi
44
Which species of Actinomyces is a/w IUD users/ PID?
Actinomyces israellii

Microbiology (19 decks)

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