Bacteriology- Staph, Strept Flashcards

(67 cards)

1
Q

Characteristics of staph aureus

A

Coagulase +
Catalase +
Produces Beta lactamases

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Main habitat of S aureus

A

Nose, skin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

All cocci are gram ____ except _____ which are gram ____

A

positive
Velonella, Neisseria, Moraxella
Negative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

All bacilli are gram ____ except _____ which are gram ____

A

negative
Mycobacteria, Anthracis, Clostridium, Diphtheria, Nocardia, Actinomyces, Listeria, Diphtheroids
positive

‘MAC DoNALD’

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Micrococcus are arranged in

A

tetrads

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Glucose utilization in micrococcus vs staphylococcus

A

In micrococcus– oxidative (micrO– Oxidative)
In staPHylococcus– Fermentative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Oxidase status in micrococcus vs staphylococcus

A

Staph- oxidase -ve
Micro- oxidase +ve

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Catalase status of staph vs micro

A

both are catalase positive

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Virulence factors for staph aureus

A
  1. Protein A
  2. Enzymes
  3. Toxins
  4. Cytolysins
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Functions of protein A

A

Antiphagocytic
Anticomplementary
Coagglutination

Binds with Fc portion of Ig

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Enzymes of S aureus

A

Catalase
Coagulase
Thermonuclease
DNAase
Phosphatase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Toxins of S aureus

A

Exotoxins which include–
-Enterotoxin
-TSST
-Exfoliative toxin
All of them act as super antigens

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Which hemolysin is asso with hot cold phenomenon?

A

Beta hemolysin (aka sphingomyelinase)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

PV toxin is a/w _____ and is a part of _____ toxin

A

MRSA
Synergohymenotropic (Gamma hemolysin + PV toxin)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Epidermolytic toxin causes _____ and its form in children ____. It also causes ____

A

SSSS
Ritter’s syndrome
TEN

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Gram positive bacilli, spore forming aerobe

A

Bacillus sp.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Gram positive bacilli, spore forming anaerobe

A

Clostridium sp

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Gram positive bacilli, non spore forming aerobe

A

Mycobacterium
Cornyebacterium
Listeria
Nocardia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Gram positive bacilli, non spore forming, anaerobe

A

Actinomyces

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Gram positive cocci, catalase +

A

Staph

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Gram positive cocci, catalase -ve

A

Strept

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Staph aureus coagulase status

A

coagulase +

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Staphylococcal Scalded skin syndrome includes what manifestations

A

TEN
Ritter’s syndrome in children
Bullous impetigo

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Characteristic feature of TSS caused by TSST

A

Sun burn rash

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Enterotoxin mediated food poisoning caused by S aureus has ____ incubation period
1-6 hrs vomiting, diarrhoea
25
What are the cytolysins of Staphylococcus sp.
Alpha Beta Gamma hemolysins Panton Valentine Leukocidins
26
Selective medium for Staph Aureus
Mannitol Salt Agar-- Golden yellow colonies Ludlam medium-- Black colonies
27
IOC for S. aureus
Culture ELISA/ PCR
28
TOC for 1. Penicillin resistant S. aureus 2. MRSA 3. VRSA 4. LRSA
1. Methicillin 2. Vancomycin 3. Linezolid 4. Daptomycin
29
Gene that confers MRSA and VRSA its resistance
MRSA- mec A gene VRSA- Van A gene
30
Treatment for TSS Food poisoning d/t S aureus
TSS- CLindamycin Food poison-- No antibiotics
31
Coagulase negative Staph classified acc to
Novobiocin sensitivity
32
CONS which is novobiocin sensitive
S. epidermidis
33
CONS which is novobiocin resistant
S. saprophyticus
34
S. epidermidis characteristics
Biofilms Skin commensal Prosthetic Valve Infective Endocarditis in < 12 mths
35
S. saprophyticus characteristics
Causes UTI, Honeymoon cystitis in young women
36
Streptococcus is classified acc to
Brown classification- Hemolysis in blood agar alpha, beta, gamma
37
Alpha hemolytic strept examples
S. pneumoniae S. viridans
38
Beta hemolytic strept is further classified acc to
Lancefield grouping (acc to carbohydrate in cell wall) A to W except I and J
39
Gamma hemolysis example
Enterococci
40
Virulent factors of S. pneumoniae
Main- Polysaccharide capsule IgA protease Pneumolysin
41
Characteristics of polysaccharide capsule of S. pneumoniae
Anti phagocytic Virulent forms Mucoid colonies Has > 95 serotypes
42
Diseases caused by pneumococcus
POEMS Pneumonia Otitis media Epiglottitis Meningitis Sinusitis
43
Lab diagnosis of pneumococcus
Quellung reaction to demonstrate capsule Carrom coin/ Draughtman colonies in Blood agar, Chocolate agar ELISA/ PCR Bile soluble Optochin sensitive
44
Treatment for Pneumococcus
Ceftriaxone Gentamicin
45
Vaccines against pneumococcus
MI indication: Splenectomy pts PCV-13 PPSV 23
46
S. viridans characteristics
commensal in oral cavity produces biofilms causes prosthetic valve endocarditis in > 12 mths a type of viridans-- S. mutans causes dental caries
47
Group A Beta hemolytic strept is aka
S. pyogenes
48
S. pyogenes capsule
Hyaluronic acid capsule
49
Exotoxin produced by S. pyogenes
Streptococcal pyrogenic exotoxin (super Ag)
50
Streptococcal pyrogenic exotoxin causes
Scarlet fever
51
Enzymes produced by Strep. pyogenes
Hyaluronidase-- spreading factor Streptokinase, Streptodornase-- thrombolytic-- used in Tx for MI, DVT
52
Virulence factors of pyogenes
Enzymes Exotoxins M proteins Streptolysin O
53
Streptolysin O
Immunogenic If ASO > 200 todd units, means recent exposure
54
M protein
Major virulence factors Griffith typing-- > 150 types
55
Suppurative lesions caused by S. pyogenes
Throat- Sore throat, pustular tonsilitis Skin- NICE Necrotizing fascitis Impetigo Cellulitis Erysipelas
56
Beta hemolytic strept
S. pyogenes S. agalactiae
57
Acute rheumatic fever occurs after ___ infection
sore throat d/t s. pyogenes
58
PSGN occurs after ___ infection
Skin infection> sore throat d/t s. pyogenes
59
Which HS reaction is ARF and PSGN?
ARF- type II HSR PSGN- type III HSR
60
Lab diagnosis of S. pyogenes
Culture in PNF medium (Polymyxin, Neomycin, Fucidic acid medium) Bacitracin sensitivity test PYR test
61
S. agalactiae
GBS Commensal in female genital tract Causes neonatal-- meningitis, sepsis, arthritis
62
Lab diagnosis of GBS
Hippurate hydrolysis + CAMP test + (CAMP factor increases beta hemolytic activity)
63
All streptococcus treatment (except pneumococcus), CONS treatment
Penicillins, cephalosporins
64
Enterococcus grows in
42 C 6.5% salt 9.6 pH 40% bile Bile esculin hydrolysis +
65
Tx for Enterococcus
Vancomycin If resistant, forms VRE--> tx: Linezolid, Daptomycin
66