Flashcards in BC - L5 Deck (13):
passive immunity & HIV
modulating the immune system
new therapeutic uses for mAbs
completed vaxine trials
HIV entry process
enriching for Abs the recognise CD4 binding site
large scale isolation of bNt mAbs
improving HIV mAbs
types of therapy tested
What happened with the completed HIV vaccine clinical trials?
no protection observed
HIV has a very large number of genetic variants circulating at the same time (compared to flu) --> Abs dont function
only bNt mAbs
Describe the HIV entry process
Abs for CD4?
delRSC3 is a mimic of virus that binds to the CD4 receptor
How to isolate bNt mAbs fro memory cells?
sort single IgG B Cells that bind a modified gp160 trimer called 2CC
recover the IgV genes from each cell
re-express as a recombinant, complete IgG in a test tube
What is the arms race, descirbe
Example of improving mAbs for HIV
G --> aromatic
Types of therapies?
mono - ineffective
tri - partly effective
penta - effective
Modulating the immune system?
T cell activation
activation of CTLA4 & PD-1 pathways in immune response
New therapeutic uses for mAbs?
anti-CD19 CAR T cell therapy