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Flashcards in BC - L5 Deck (13):
1

Topics?

passive immunity & HIV
modulating the immune system
new therapeutic uses for mAbs

2

HIV subtopics?

completed vaxine trials
selection..
HIV entry process
enriching for Abs the recognise CD4 binding site
large scale isolation of bNt mAbs
arms race
improving HIV mAbs
types of therapy tested

3

What happened with the completed HIV vaccine clinical trials?

no protection observed

4

selection?

HIV has a very large number of genetic variants circulating at the same time (compared to flu) --> Abs dont function
only bNt mAbs

5

Describe the HIV entry process

-

6

Abs for CD4?

delRSC3 is a mimic of virus that binds to the CD4 receptor

7

How to isolate bNt mAbs fro memory cells?

sort single IgG B Cells that bind a modified gp160 trimer called 2CC
recover the IgV genes from each cell
re-express as a recombinant, complete IgG in a test tube

8

What is the arms race, descirbe

-

9

Example of improving mAbs for HIV

G --> aromatic

10

Types of therapies?

mono - ineffective
tri - partly effective
penta - effective

11

Modulating the immune system?

Ipilimumab
T cell activation
activation of CTLA4 & PD-1 pathways in immune response

12

New therapeutic uses for mAbs?

CAR
anti-CD19 CAR T cell therapy

13

Describe the CAR pathway

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