Flashcards in BC - L4 Deck (13):
how mAbs are made
mechanisms of action
limitations of mAbs
What are monoclonal antibodies? Why do they have great therapeutic potential?
a pure population of antibodies with one target specificity made by a clonal population of antibody secreting plasma cells
extremely well tolerated
very specific & selective
Describe how the first mAbs were made
Humanisation of mAbs?
to avoid HAMA
fully mouse (-o-)
replace mouse IgH & IgL C-region with human (-xi-)
graft mouse VH & VL CDR onto human framework (-zu-)
graft one V CDR, other V chimeric (-xizu-)
only human, XenoMouse (-u-)
Describe new generation mAb pathway
Describe humanisation via isolation
Describe humanisation using bacteriophage display
Mechanisms of action?
target cell activation
ligand blockade examples? how they work? (3)
anti-TNFa (infliximab, adalimumab)
receptor blockade examples? how they work? (1)
how does target cell activation work?
T cell activating