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Flashcards in BC - L4 Deck (13):
1

Topics discussed?

about mAbs
how mAbs are made
mechanisms of action
limitations of mAbs

2

What are monoclonal antibodies? Why do they have great therapeutic potential?

a pure population of antibodies with one target specificity made by a clonal population of antibody secreting plasma cells

extremely well tolerated
long lived
very specific & selective

3

Describe how the first mAbs were made

-

4

Humanisation of mAbs?

to avoid HAMA
fully mouse (-o-)
replace mouse IgH & IgL C-region with human (-xi-)
graft mouse VH & VL CDR onto human framework (-zu-)
graft one V CDR, other V chimeric (-xizu-)
only human, XenoMouse (-u-)

5

Describe new generation mAb pathway

-

6

Describe humanisation via isolation

-

7

Describe humanisation using bacteriophage display

-

8

Mechanisms of action?

ligand blockade
receptor blockade
depletion
target cell activation

9

ligand blockade examples? how they work? (3)

anti-TNFa (infliximab, adalimumab)
anti-VEGFA (bevacizumab)
anti-RANKL

10

receptor blockade examples? how they work? (1)

anti-HER2 (Trastuzumab)

11

depletion examples?

anti-CD20 (Rituximab)

12

how does target cell activation work?

T cell activating

13

limitations?

unpredictable effects
cardiotoxicity
infections
acute anaphylaxis
generation of anti-mAb antibodies