biotech <-> my own notes Flashcards
(16 cards)
the manipulation of living organisms or their
components to produce useful products
biotechnolgy
applied science that explores applications of cutting, splicing, and creating DNA
Recombinant DNA technology
GENETIC ENGINEERING
MAINPULATION OF THE genetic makeup of cells or whole organisms
determines the precise sequence of
bases, adenine (A), cytosine (C), guanine (G), and
thymine (T), in a segment of DNA or in the whole
genome
dna seqeuncing
newer dna seqeuncing methods (automated)
- deep sequencing
- massdivily parallel seqeuncing
- high-thoughput sequencing
tools for manipulating dna
Restriction enzymes: cut DNA at specific sites
* DNA ligases: join fragments of DNA
* Plasmids: small circular pieces of DNA to which desired
genes can be added and inserted into bacteria for
amplification
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CRISPR-Cas9
system enables the nucleotide
sequence of a gene to be changed within a living cell
can be used to rapidly amplify specific DNA
sequences to obtain millions of copies
PCR 9(The polymarase Chain reaction )
identifying the source of dna
dna profiling
an organism that has been genetically
engineered to carry one or more “foreign” genes from another species
transgenic organism
Monoclonal Antibodies
Laboratory-
Created for Commercial Use
introduction of human genes into human cells to treat or
eliminate a disease
gene therapy
how can dna be introduced into human cells
viral vectors - human gene can be packaged into viruses; ex vivo and in vivo
ex vivo gene therpay
Viruses carrying the human gene
of interest are introduced into cells that have been
removed from the body. The infected cells are then
returned to the patient
in vivo gene therapy
viruses carrying the human gene
of interest are injected directly into the patient—even
directly into the desired tissue
small fatty spheres surrounding the gene
liposomes
