Flashcards in C diff Deck (13):
What is the general classification of C diff?
gram +, rod (bacilli), anaerobic, spore forming
Two toxins associated with C diff?
Toxin A: enterotoxin
Toxin B: cytotoxin Hyaluronidase
What is the "gold standard" for C diff testing?
other than cytotoxin assay, what is an important test for C diff?
ELISA- enzyme linked immunosorbent
-tests for both toxin A and B
-low sensitivity (60-80%)
What is the mechanism of action for toxin A?
(enterotoxin) activates inflammatory cascade in colon, disrupts the intercellular tight junctions causing fluid secretion, mucosal injury, edema, and inlammation
What is the mechanism of action for toxin B?
(cytotoxin) disrupts cytoskeleton, leading to mucosal injury and activation of inflammatory cascade
Occurs prior to sterilization, doesn't remove microbes but removes chemicals, radioactivity to make safe to handle
destroys all living organisms, viruses, and endospores so they are no linger able to reproduce
reduces organisms to a low enough level that disease is unlikely; inanimate objects (ie. bedpans) since often too toxic to use on human tissue
microbicide safe to use in human tissue
decrease number of microbes to a safe level but doesn't eliminate
procedure performed under sterile conditions