Week 3 Diebel Review Flashcards Preview

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Flashcards in Week 3 Diebel Review Deck (17):
1

How does bacterial staining differentiate between Gram + and - ?

Gram + --> purple, thick peptidiglycan cell wall

Gram - --> pink, thin peptidoglycan layer between inner and outer membrane

2

What are the virulence factors of Salmonella?

O antigen--inhinits phagocyte killing

anti-phagocyte protein induced by ocyR

LPS--endotoxin, produces fever

Injectosome--help hide?

Enterotoxin--diarrhea

Type 1 fimbriae--adherence

Cytotoxin--inhibits host cell protein synthesis

Vi Capsule antigen- inhibits complement binding

Flagellum--motility

H antigen--adherence

3

Contrast lytic and lysogenic bacteriophages.

lytic destroys host cell

lysogenic/temperate does not

4

What structures are common among all viruses?

genetic material and capsid

5

genetic info and replication for Class 1?

dsDNA (+/-), semiconservative replication of minus strand

6

genetic info and replication for Class 2?

ssDNA (+), synthesis of dsDNA intermediate, semiconservative, discards (-) strand

7

genetic info and replication for Class 7?

dsDNA (+/-), transcription followed by reverse transcription

8

genetic info and replication for Class 3?

dsRNA (+/-), makes ssRNA (+) and transcribe from this to give ssRNA (-) partner

9

genetic info and replication for Class 4?

ssRNA (+), makes ssRNA (-) and transcribe from this to give ssRNA (+) partner

10

genetic info and replication for Class 5?

ssRNA (-), makes ssRNA (+) and transcribe from this to give ssRNA (-)

11

genetic info and replication for Class 6?

ssRNA (+), makes ssRNA (+) by transcription of (-) strand of dsDNA

12

What are the differences between the innate and adaptive immune systems?

Innate is ready to go all the time
-macrophages and dendritic cells

Adaptive must be "trained" in lymphocytes
-T cells and B cells
-has memory
-quicker and stronger response to reexposure

13

What type of cells are first response in tissue?

macrophages and dendritic cells

14

what is opsonization?

the antibody marks a pathogen for destruction by a phagocyte

15

what are the methods for bacterial genetic exchange?

Homologous recombination-cross strand exchange from one chromosome to another
Transformation-free DNA is picked up by a competent host cell
Transduction (generalized and specialized)-transfer of DNA from one cell to another via bacteriophage
Conjugation-requires cell to cell contact transfer through a pilus
Transposition-discrete segments of DNA move as a unit from one location to another within in other DNA moleculs

16

What is the virulence factor that HCV uses to shut off intracellular innate immunity? how does it work?

NS3/4A, blocks TRIF and MAVS of host cell which are in charge of creating IFN-B

17

What are selective and differential medias and how do they function in general?

Selective: contains compounds that selectively inhibits growth of some microbes but not others

Differential: contains an indicator, usually a dye, that detects particular chemical reactions occurring during growth