Flashcards in Week 3 Diebel Review Deck (17):
How does bacterial staining differentiate between Gram + and - ?
Gram + --> purple, thick peptidiglycan cell wall
Gram - --> pink, thin peptidoglycan layer between inner and outer membrane
What are the virulence factors of Salmonella?
O antigen--inhinits phagocyte killing
anti-phagocyte protein induced by ocyR
LPS--endotoxin, produces fever
Type 1 fimbriae--adherence
Cytotoxin--inhibits host cell protein synthesis
Vi Capsule antigen- inhibits complement binding
Contrast lytic and lysogenic bacteriophages.
lytic destroys host cell
lysogenic/temperate does not
What structures are common among all viruses?
genetic material and capsid
genetic info and replication for Class 1?
dsDNA (+/-), semiconservative replication of minus strand
genetic info and replication for Class 2?
ssDNA (+), synthesis of dsDNA intermediate, semiconservative, discards (-) strand
genetic info and replication for Class 7?
dsDNA (+/-), transcription followed by reverse transcription
genetic info and replication for Class 3?
dsRNA (+/-), makes ssRNA (+) and transcribe from this to give ssRNA (-) partner
genetic info and replication for Class 4?
ssRNA (+), makes ssRNA (-) and transcribe from this to give ssRNA (+) partner
genetic info and replication for Class 5?
ssRNA (-), makes ssRNA (+) and transcribe from this to give ssRNA (-)
genetic info and replication for Class 6?
ssRNA (+), makes ssRNA (+) by transcription of (-) strand of dsDNA
What are the differences between the innate and adaptive immune systems?
Innate is ready to go all the time
-macrophages and dendritic cells
Adaptive must be "trained" in lymphocytes
-T cells and B cells
-quicker and stronger response to reexposure
What type of cells are first response in tissue?
macrophages and dendritic cells
what is opsonization?
the antibody marks a pathogen for destruction by a phagocyte
what are the methods for bacterial genetic exchange?
Homologous recombination-cross strand exchange from one chromosome to another
Transformation-free DNA is picked up by a competent host cell
Transduction (generalized and specialized)-transfer of DNA from one cell to another via bacteriophage
Conjugation-requires cell to cell contact transfer through a pilus
Transposition-discrete segments of DNA move as a unit from one location to another within in other DNA moleculs
What is the virulence factor that HCV uses to shut off intracellular innate immunity? how does it work?
NS3/4A, blocks TRIF and MAVS of host cell which are in charge of creating IFN-B