Flashcards in C10 Manipulating genes and cells Deck (16)
Most animal cells cannot grow indefinitely due to the loss of ?
Most animal cells cannot grow indefinitely with the exception of ?
embryonic stem cells
molecular scissors for DNA
"dideoxy" method developed by..
southern blotting procedure
electrophoresis of DNA cut with restriction enzymes. blot gel onto nitrocellulose. allow labeled DNA probe to hydridize to specific sequence on nitrocellulose.
? hybridization can locate genes on chromosomes
? are commonly used as cloning vectors
DNA created by reverse transcription of mRNA
Problem with cDNA library
produces more DNA for those genes transcribed more often
synthetic primer containing desired mutated sequence binds to nearly complementary sequence. When duplicated, two different DNA sequences are created.
Single stranded DNA sequence used in hybridization to detect certain sequences
joins DNA fragments together to produce a recombinant DNa molecule
Causes most common type of hemophilia
most common was to rapidly sequence DNA