C10 Manipulating genes and cells Flashcards Preview

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Flashcards in C10 Manipulating genes and cells Deck (16):
1

Most animal cells cannot grow indefinitely due to the loss of ?

telomerase

2

Most animal cells cannot grow indefinitely with the exception of ?

embryonic stem cells

3

molecular scissors for DNA

Restriction enzymes

4

"dideoxy" method developed by..

Sanger

5

southern blotting procedure

electrophoresis of DNA cut with restriction enzymes. blot gel onto nitrocellulose. allow labeled DNA probe to hydridize to specific sequence on nitrocellulose.

6

? hybridization can locate genes on chromosomes

in situ

7

? are commonly used as cloning vectors

bacterial plasmids

8

cDNA

DNA created by reverse transcription of mRNA

9

Problem with cDNA library

produces more DNA for those genes transcribed more often

10

in vitro

in glass

11

site-directed mutagenesis

synthetic primer containing desired mutated sequence binds to nearly complementary sequence. When duplicated, two different DNA sequences are created.

12

Single stranded DNA sequence used in hybridization to detect certain sequences

DNA probe

13

joins DNA fragments together to produce a recombinant DNa molecule

ligase

14

Causes most common type of hemophilia

factor 8

15

most common was to rapidly sequence DNA

dideoxy sequencing

16

regulatory regions upstream of coding region can be made to control expression of a ? whose expression can be easily monitored

reporter gene