Flashcards in C7 DNA to Protein Deck (32):
What is a promoter
Location of the initiation of synthesis
Three steps in Nucleic acid polymerization
Initiation, elongation, termination
Bacterial RNAP uses this to help recognize the DNA sequence of a promoter
Has polycistronic DNA (eukarya vs prokarya)
In prokaryotes, it directs RNAP to start at different promoter sequences
number of RNA polymerases in prokaryotes
number of RNA polymerases in eukaryotes
Which RNAP makes rRNA in eukaryotes
Which RNAP makes tRNA and snRNA in eukaryotes
Which RNAP makes mRNA in eukaryotes
Requires multiple general transcription factors to form a transcription initiation complex
The TBP region of this transcription factor binds to the TATA box within the promoter
What occurs when RNAP II transitions from initiation to elongation?
Initiation factors release and C-terminal domain of RNAP is phosphorylated
Why does thymine in DNA have less error than Uracil in RNA?
cytosine can be deaminated to uracil. Easily recognized in DNA as error. More difficult in RNA.
In eukaryotes, transcription occurs here
What is added to the 5' end of a primary transcript?
How does the 5cap and PolyAtail effect the mRNA?
Allows it to be transported out of the nucleus and increases its stability
Single coding sequence that codes for multiple proteins
Splicing is controlled by a ? complex known as a ?. It contains ?
ribonucleoprotein. spliceosome. snRNPs
Why have splicing?
Additional flexibility in recombination
snRNPs are made up of.. (2)
protein and snRNA
Enzyme that catalyzes charging of tRNAs with respective amino acid
Two types of Ribosomes
attached to rough ER and free ribosomes
Prokaryote ribosome binding site
proteins are broken down by ? often in a ?
Signal recognized by proteasome
Bacteria Promoter Stuctures
-35 and -10 sequences and sigma factors
Eukaryote Promoter Structures
TATA Box and general transcription factors
Bacteria Ribosome mRNA binding structures
Shine dalgarno and AUG
Eukaryote Ribosome mRNA binding structures
5'cap and AUG