C7 DNA to Protein Flashcards Preview

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Flashcards in C7 DNA to Protein Deck (32):
1

What is a promoter

Location of the initiation of synthesis

2

Three steps in Nucleic acid polymerization

Initiation, elongation, termination

3

Bacterial RNAP uses this to help recognize the DNA sequence of a promoter

sigma factor

4

Has polycistronic DNA (eukarya vs prokarya)

prokarya

5

In prokaryotes, it directs RNAP to start at different promoter sequences

sigma factor

6

number of RNA polymerases in prokaryotes

1

7

number of RNA polymerases in eukaryotes

3

8

Which RNAP makes rRNA in eukaryotes

pol I

9

Which RNAP makes tRNA and snRNA in eukaryotes

pol III

10

Which RNAP makes mRNA in eukaryotes

pol II

11

Requires multiple general transcription factors to form a transcription initiation complex

RNAP 2

12

The TBP region of this transcription factor binds to the TATA box within the promoter

TFIID

13

What occurs when RNAP II transitions from initiation to elongation?

Initiation factors release and C-terminal domain of RNAP is phosphorylated

14

Why does thymine in DNA have less error than Uracil in RNA?

cytosine can be deaminated to uracil. Easily recognized in DNA as error. More difficult in RNA.

15

In eukaryotes, transcription occurs here

nucleus

16

What is added to the 5' end of a primary transcript?

7-methyl-G

17

How does the 5cap and PolyAtail effect the mRNA?

Allows it to be transported out of the nucleus and increases its stability

18

Single coding sequence that codes for multiple proteins

polycistronic

19

Splicing is controlled by a ? complex known as a ?. It contains ?

ribonucleoprotein. spliceosome. snRNPs

20

Why have splicing?

Additional flexibility in recombination

21

snRNPs are made up of.. (2)

protein and snRNA

22

Enzyme that catalyzes charging of tRNAs with respective amino acid

aminoacyl-tRNA syntetases

23

Two types of Ribosomes

attached to rough ER and free ribosomes

24

Prokaryote ribosome binding site

shine dalgarno

25

proteins are broken down by ? often in a ?

proteases, proteasome

26

Signal recognized by proteasome

ubiquitin

27

RNA enzymes

ribozymes

28

Bacteria Promoter Stuctures

-35 and -10 sequences and sigma factors

29

Eukaryote Promoter Structures

TATA Box and general transcription factors

30

Bacteria Ribosome mRNA binding structures

Shine dalgarno and AUG

31

Eukaryote Ribosome mRNA binding structures

5'cap and AUG

32

Describe eukaryotic translation initiation

initiator tRNA and initiation factors bind to small ribosome subunit. Snall subunit binds to mRNA. Initiator tRNA finds AUG start site. Initiation factors dissociate. Large ribosome subunit binds.