Catabolic Reactions Flashcards

(34 cards)

1
Q

What is the process for ethanol fermentation?

A

pyruvate turns into acetaldehyde and CO2
acetaldehyde turns into ethanol
NADH turns to NAD

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2
Q

When is ethanol fermentation used?

A

yeast in the absence of oxygen
ethanol used to make beer and wine

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3
Q

What is the process of lactic acid fermentation?

A

pyruvate turns into lactate (reversible)
NADH turns to NAD

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4
Q

When is lactic acid fermentation used?

A

certain bacteria utilized in food production (yogurt, sour cream, kimchee)
humans do this when there is no oxygen
ex) Lactobacilius sp. , Streptocoocus sp.

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5
Q

What is the process of mixed acid fermentation and an example?

A

pyruvate turns to formate which turns into H2+CO2
NADH turns into NAD
ex) E.coli

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6
Q

What is the process of butanediol fermentation?

A

pyruvate to acetoin to 2,3 butanediol
NADH turns into NAD
ex) Enterobacter aerogenes

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7
Q

What is a phosphoroclastic reaction?

A

Pyruvate + CoA eventually creates
Acetyl PO4 +ADP which makes acetate and ATP
ex) Clostridium sp.

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8
Q

What is ferriddoxin?

A

an iron contain protein is responsive for H2 production in a phosphoroclastic reaction

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9
Q

What are other substrates that can undergo fermentation other than glucose?

A

sugars other than glucose, amino acids, aromatic compounds, and purines/pyrimidines

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10
Q

What is the preparation for the Krebs cycle and where does it occur?

A

pyruvic acid turns into acetyl CoA and CO2
1 NADH formed per pyruvate conversion
occurs in the mitochondria

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11
Q

What is the rest of the Krebs cycle?

A

Acetyl group + oxaloacetic acid turns into citric acid
citric acid after 7 steps turns into oxaloacetic acid
3 NADH, 1 FADH2, and 1 GTP formed per acetyl group
releases CO2

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12
Q

What is the glyoxylate cycle?

A

Catabolism of C2-C3 organic acids typically involves production of oxaloacetate
Glyoxylate is a key intermediate

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13
Q

What is the electron transport system?

A

uses NADH and FADH2 to move H+ ions from matrix to outside of the membrane
Movement of H+ ions down concentration gradient provides energy to make ATP from ADP and P

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14
Q

What is the terminal electron acceptor in the electron transport system?

A

oxygen

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15
Q

Where does glycolysis occur?

A

in the cytoplasm

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16
Q

Why is there a reduction in net ATP for eukaryotes?

A

have to transfer the pyruvate in to the mitochondria costs 2-4 ATP

17
Q

What is unique about anaerobic respiration?

A

use of electron acceptors other than oxygen
less energy released compared to aerobic respiration
ex) NO3, SO4, CO3

18
Q

Nitrate anaerobic respiration

A

NO3 to N2O to N2
Facultative anaerobes
ex) Pseudomonas sp., Bacillus sp., Moraxella sp.

19
Q

Sulfate anaerobic respiration

A

SO4 to S to H2S
Strict anaerobe
ex) Desulfovibrio sp.

20
Q

What microbes use fumarate to succinate (TCA Cycle)?

A

E coli, Proteus sp., Enterococcus sp.

21
Q

Methane anaerobic respiration

A

CO2 to CH4
Methanogenic bacteria found in intestinal tracts, sewage plants, ruminants
Methanobacter sp., Methanococcus sp.

22
Q

Iron anaerobic respiration

A

Fe3+ to Fe2+
Acidophilic (H2SO4)
ex) thiobacillus feroxidans

23
Q

What is chemolithotrophy?

A

uses inorganic chemicals as electron donors
typically aerobic
begins with oxidatoin of inorganic electron donor

24
Q

What is phototrophy?

A

light as energy source

25
What is photophosphorylation?
light-mediated ATP synthesis
26
What is photoautotrophs?
use ATP for assimilation of CO2 for biosynthesis
27
What are photoheterotrophs?
use assimilation of organic carbon for biosynthesis
28
What enzyme converts cellulose to glucose?
cellulase ex: Clostridium sp.
29
What enzyme converts starch to glucose?
amylase ex: Bacillus
30
What enzyme converts lactose to glucose and galactose?
B-galactosidase ex: E.coli
31
What does starch agar test?
if amylase is produced, then the starch will be degraded
32
What enzyme converts triglycerides to glycerol and fatty acids?
lipase ex: Staphylococcus aureus media: spirit blue agar
33
What enzyme converts phospholipids to phosphorylcholine and fatty acids
phospholipase ex: Clostridium perfringens media: egg yolk agar
34
What enzyme converts proteins to amino acids?
protease ex: serratia marcescer media: skim milk agar