CC2 LAB: CREATININE KINASE, ALT, AST

Question 1

associated with atp regeneration in contractile or transport system

Answer 1

creatinine kinase

Question 2

ck is predominant in

Answer 2

muscle cells

Question 3

how many kDa in ck

Answer 3

82

Question 4

separation of atypical bands
detection of ak

Answer 4

electrophoresis (reference method)

Question 5

good with technique, may be more sensitive and precise

Answer 5

ion-exchange chromatography

Question 6

measure concentration rather than activity
may detect inactive ck-mb

Answer 6

radioimmunoassay

Question 7

• anti-M inhibits M activity
• CK activity is measure before and after inhibition
• residual activity is multiplied by 2 to account for MB
  (50% inhibited)
• disadvantage: detects BB activity; though small amount,
  may cause false MB activity; also, atypical forms are not
  inhibited

Answer 7

immunoinhibition

Question 8

A in Coupled-enzyme method

Answer 8

340 nm

Question 9

forward reaction in ck

Answer 9

Tanzer-Gilvarg

Question 10

• reverse reaction in ck
• most common
• 2 – 6x faster than forward
• less interference

Answer 10

Oliver Rosalki

Question 11

Tanzer-Gilvarg Method pH

Answer 11

pH 9.0

Question 12

Oliver-Rosalki Method pH

Answer 12

pH 6.8

Question 13

inactivated because of oxidation of sulfhydryl groups

Answer 13

CK activity unstable

Question 14

T/F: Hemolyzed samples falsely elevate CK activity

Answer 14

T

Question 15

ratio of ck

Answer 15

1:4

Question 16

working reagent (microliter) ck & ck-mb

Answer 16

1000uL

Question 17

sample (microliter) ck & ck-mb

Answer 17

40uL

Question 18

Transfer of an amino group
between aspartate and alpha-
keto acids to form oxaloacetate and glutamate

Answer 18

Aspartate Aminotransferase

Question 19

coenzyme of ast

Answer 19

Pyridoxal phosphate

Question 20

former name of ast

Answer 20

serum glutamic-oxaloacetic
transaminase

Question 21

Consists of a coupled enzymatic reaction using Lactate Dehydrogenase as the
indicator enzyme

Answer 21

alt

Question 21

Transfer of an amino group
between alanine and alpha-
keto acids to form glutamate
and pyruvate

Answer 21

Alanine Aminotransferase

Question 22

coenzyme of alt

Answer 22

Pyridoxal phosphate

Question 22

former name of alt

Answer 22

serum glutamic-pyruvic
transaminase

Question 23

Catalyzes the reduction of pyruvate to lactate with simultaneously oxidation of NADH

Answer 23

alt

Question 24

optimal pH of alt

Answer 24

7.3 – 7.8

Question 25

Relatively unaffected by hemolysis

Answer 25

alt

Question 26

sample of ast in microliter

Answer 26

100

Question 27

coupled enzymatic reaction ▪ AST and malate dehydrogenase (MD) as the indicator reaction ▪ change in absorbance at 340 nm as NADH is oxidized to NAD+

Answer 27

Karmen Method

Question 27

Optimal pH of ast

Answer 27

7.3 – 7.8

Question 28

reaction with ... couples color reagent with keto acid product

Answer 28

dinitrophenylhydrazine

Question 28

Hemolysis should be avoided

Answer 28

ast

Question 28

reaction with ... couples the salt with the keto acid product and forms a color

Answer 28

diazonium salts