PHOSPHATASES

Question 1

from what class is alkaline phosphatase

Answer 1

hydrolases

Question 2

systematic name of alp

Answer 2

Orthophosphoric Monoester
Phosphohydrolase (Alkaline
Optimum)

Question 3

product of alp

Answer 3

alcohol
phosphate ion

Question 3

optimum pH of alp

Answer 3

9.0 -10.0

Question 4

activators of alp

Answer 4

magnesium
zinc

Question 4

substrate of bessey-lowry-brock method

Answer 4

p-nitrophenylphosphate

Question 5

p-nitrophenyl phosphate (colorless) is HYDROLYZED to

Answer 5

p-nitrophenol (yellow)

Question 5

Calculation of ALP activity based on the molar absorptivity of p-nitrophenol

Answer 5

BOWERS AND MCCOMB

Question 6

CONTINUOUS-MONITORING TECHNIQUE is …proportional to alp activity

Answer 6

DIRECTLY PROPORTIONAL

Question 6

tissue sources of alp

Answer 6

liver
bone
kidney
spleen
intestine
placenta

Question 6

absorbance of alp

Answer 6

405 nm

Question 6

major tissue source of acp

Answer 6

prostate

Question 7

most anodal furthest to cathode

Answer 7

liver alp

Question 8

most heat labile

Answer 8

bone alp

Question 9

least heat labile

Answer 9

placenta alp

Question 10

least anodal nearest to cathode

Answer 10

intestinal alp

Question 10

isoenzymes of acp

Answer 10

prostatic
lysosomal
macrophage
erythrocytic
osteoclastic

Question 11

elevated during adenocarcinoma of the pancreas

Answer 11

nagao alp

Question 12

most heat stable alp (in general)

Answer 12

regan alp

Question 13

abundant in cancers affecting the lungs, breasts, and gynecological areas

Answer 13

regan alp

Question 14

important in differentiating bone alp and regan alp

Answer 14

phenylalanine reagent

Question 15

major source of acp

Answer 15

prostate

Question 16

what sample that men produce where acp is found

Answer 16

semen

Question 17

most common method of measuring alp kinetic method

Answer 17

bowers and mccomb

Question 18

substrate of bowers and mccomb

Answer 18

para-nitrophenylphosphate

Question 19

ph of P-npp

Answer 19

10.2

Question 20

products of para-nitrophenylphosphate

Answer 20

phosphate ion para-nitrophenol

Question 21

what color is para-nitrophenol after is changed its absorbance

Answer 21

yellow

Question 22

inactivates alp

Answer 22

phosphorus

Question 23

prevents the inactivation of alp

Answer 23

2-amino-2-methyl-1-propanol

Question 24

result of hemolysis in acp

Answer 24

falsely increase

Question 25

inhibits intestinal and placental alp to a much greater extent than liver and bone alp

Answer 25

phenylalanine

Question 26

substrate of choice for quantitative endpoint reactions

Answer 26

thymolphthalein monophosphate

Question 27

substrate choice for continuous monitoring methods

Answer 27

alpha-napththyl phosphate

Question 28

inhibit phosphorus present in serum + mg/zinc in serum

Answer 28

2-amino-2-methyl-propanolol

Question 29

blood type that binds to erythrocytes

Answer 29

type a

Question 30

carcinoplacental isoenzymes

Answer 30

regan nagao kasahara

Question 31

substrate of acp

Answer 31

phosphomonoesters

Question 32

assay that does not need of serum to acidify

Answer 32

radioimmunoassay