CC2 LAB: LACTATE DEHYDROGENASE AND CREATININE KINASE

Question 1

catalyzes the interconversion of lactic and pyruvic acid

Answer 1

lactate dehydrogenase

Question 2

from what major class is lactate dehydrogenase

Answer 2

oxidoreductase

Question 3

how many daltons does ld have

Answer 3

128 kDa

Question 4

how many subunits are there in lactate dehydrogenase

Answer 4

4 (tetrameric)

Question 5

arrangement of isoenzymes

Answer 5

LDH1
LDH2
LDH3
LDH4
LDH5

Question 6

abnormal isoenzyme

Answer 6

LDH6

Question 7

tissue source for LDH1 to LDH5

Answer 7

LDH1&2: heart, rbc
LDH3: lung, lymohocyte, spleen, pancreas
LDH4: liver
LDH5: skeletal muscle

Question 8

related to heart diseases

Answer 8

LDH1

Question 9

related to hepatic disorders

Answer 9

LDH5

Question 10

most ubiquitous out of all the LDH units

Answer 10

LDH3

Question 11

serum row of a normal person

Answer 11

LDH2>LDH1>LDH3>LDH4>LDH5

Question 12

heart row of a normal person

Answer 12

LDH1>LDH2>LDH3>LDH4>LDH5

Question 13

substrate of lactate dehydrogenase

Answer 13

alpha-hydroxybutyrate

Question 14

in electrophoresis the complex is made up of

Answer 14

IgA
IgG

Question 15

what do you call the forward method and what is its optimal pH

Answer 15

Wackers method: 8.3-8.9pH

Question 16

what do you call the backward method and what is its optimal pH

Answer 16

wroblewski la due method: 7.1-7.9pH

Question 16

LD should be stored at RT within

Answer 16

48hrs

Question 16

most labile LD

Answer 16

LD-5

Question 16

associated with atp regeneration in contractile or transport system

Answer 16

creatinine kinase

Question 16

LD isoenzyme analysis should be stored at RT and analyzed within

Answer 16

24hrs of collection

Question 17

LDH changes after how many days of storage of serum at rt

Answer 17

3 days

Question 17

from what major class is creatinine kinase

Answer 17

transferases

Question 17

ck3

Answer 17

cardiac and skeletal muscles

Question 18

ck1

Answer 18

brain

Question 18

ck2

Answer 18

cardiac muscles

Question 19

fastest to migrate toward anode

Answer 19

ck1

Question 20

slowest mobility

Answer 20

ck3

Question 21

major isoenzyme in the sera of healthy people that is located in the striated muscles

Answer 21

ck3

Question 22

present in small quantities in the sera of healthy people

Answer 22

ck1

Question 23

undetectable to trace in healthy people

Answer 23

ck2

Question 24

detection of AK in hemolyzed samples

Answer 24

electrophoresis

Question 25

migrate to a position midway between ck-mb and ck-mm

Answer 25

adenylate kinase

Question 26

migrate to a point cathodal to ck-mm

Answer 26

mitochondrial-ck

Question 27

exhange of ions between columns or is separated into columns

Answer 27

ion-exchange chromatography

Question 28

coupled-enzyme activity is a change in A at what absorbance

Answer 28

340 nm

Question 29

measure concentration rather than activity

Answer 29

radioimmunoassay

Question 30

forward reaction in cea and what pH

Answer 30

tanzer-gilvarg method 9.0pH

Question 31

reverse reaction in cea and what pH

Answer 31

oliver rosalki method 6.8pH