CC2 LAB: LIPASE AND AMYLASE

Question 1

to catalyze oxidation/reduction reactions

Answer 1

oxidoreductases

Question 2

to transfer functional group from one substance to another

Answer 2

transferases

Question 3

formation of 2 products from a substrate by hydrolysis using water

Answer 3

hydrolases

Question 4

intramolecular rearrangements

Answer 4

isomerases

Question 4

non-hydrolytic addition or removal of groups from substrates

Answer 4

lyases

Question 5

join together 2 molecules by the synthesis of new bonds

Answer 5

ligases

Question 6

lipase is from what major class of enzymes

Answer 6

hydrolases

Question 6

systematic name of lipase

Answer 6

triacylglycerol acylhydrolase

Question 7

specific enzymes inside the pancreas

Answer 7

lipase
amylase

Question 8

substrate of lipase

Answer 8

fats

Question 9

products of lipase

Answer 9

alcohol
fatty acids

Question 9

activators of lipase

Answer 9

colipase
bile salts

Question 9

what does lipase attacks?

Answer 9

ester linkages

Question 10

classic-cherry crandall method modified substrate

Answer 10

triolein

Question 10

lipase acute pancreatitis: onset

Answer 10

4-8 hours

Question 11

lipase acute pancreatitis: normalize

Answer 11

8-14 days

Question 11

lipase acute pancreatitis: peak

Answer 11

24 hours

Question 12

lipase assay optimal ph

Answer 12

8.8 pH

Question 12

lipase assay normal temp

Answer 12

40 degrees celsius

Question 13

what methods does lipase assay do

Answer 13

titration
turbidimetric method

Question 14

classic-cherry crandall method substrate

Answer 14

olive oil

Question 14

what does the method classic-cherry crandall used

Answer 14

titration

Question 15

in lipase what enzymes are based on coupled reactions

Answer 15

peroxidase
glycerol kinase

Question 16

in what sample or specimen is the lipase stable

Answer 16

serum

Question 17

how long does the lipase last if left in room temp

Answer 17

1 week

Question 18

how long does the lipase last if left in the refrigerator at 4 degrees celsius

Answer 18

3 weeks

Question 19

what will be the result of there is hemolysis in the sample

Answer 19

false low results

Question 20

the amylase is from what major class of enzymes

Answer 20

hydrolase

Question 20

reference ranges of lipase

Answer 20

0-1.0 U/mL 37 degrees celsius <0.6 ukat/L

Question 20

catalyzes the breakdown of starch and glycogen to monosaccharides

Answer 20

amylase

Question 21

hydrolizes the ester linkages of fats to produce alcohols and fatty acids

Answer 21

lipase

Question 22

tissue sources of amylase

Answer 22

salivary glands acinar cells of pancreas

Question 23

is a polysaccharide, made up of multiple glucose

Answer 23

starch

Question 23

long and unbranched chain linked by a-1,4 glycosidic bonds

Answer 23

amylose

Question 24

branched chain polysaccharide with a-1,6 linkages

Answer 24

amylopectine

Question 25

substrate of amylase

Answer 25

starch/glycogen

Question 25

storage for of glucose

Answer 25

glycogen

Question 26

product of amylase

Answer 26

monosaccharides: -glucose -maltose -dextrin

Question 27

activator of amylase

Answer 27

calcium ions chloride

Question 28

what does amylase attacks

Answer 28

a-1,4 glycosidic bonds

Question 28

amylase acute pancreatitis: onset

Answer 28

5-8 hours

Question 29

amylase acute pancreatitis: peak

Answer 29

24 hours

Question 30

amylase acute pancreatitis: normalize

Answer 30

2-5 days

Question 31

measures the disappearance of starch substrate

Answer 31

amyloclastic

Question 32

measures the appearance of the product

Answer 32

saccharogenic

Question 33

coupling of several enzyme systems to monitor amylase activity

Answer 33

continuous monitoring

Question 33

measures the increasing color from production of product coupled with a chromogenuc dye

Answer 33

chromogenic

Question 33

what color does amyloclastic gives

Answer 33

dark-blue

Question 34

amylase coupled enzyme absorbance of NADH is at

Answer 34

@340 nm

Question 35

optimum pH of amylase

Answer 35

6.9-7.0

Question 36

absorbance of NAD+

Answer 36

@260 nm

Question 37

absorbance of NADH

Answer 37

@340 nm

Question 38

inhibits salivary amylase

Answer 38

wheat germ lectin

Question 38

amylase is stable in what samples

Answer 38

serum urine

Question 39

the samples of amylase in rt can last up to

Answer 39

1 week

Question 40

the samples of amylase in 4 degrees celsius can last up to

Answer 40

2 months or 6 months

Question 41

you can detect acute pancreatitis in what enzyme

Answer 41

amylase lipase

Question 42

anticoagulants used in amylase

Answer 42

citrate edta oxalate

Question 43

capacity of the machine to read the sample

Answer 43

linearity

Question 44

measurement of true negative

Answer 44

specificity

Question 45

measurement of true positive

Answer 45

sensitivity