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Fundamentals in Blood Bank > Compatibility Testing > Flashcards

Flashcards in Compatibility Testing Deck (17):
1

Consist of mixing donors serum to patients red cell

Minor cross matching

2

True or false it is necessary to perform a minor cross match because donor units are screened for unexpected antibodies

False it is no longer perform

3

Give the 6 causes of positive results in the major crossmatching

1. Incorrect ABO grouping of the patient or donor
2. An alloantibody in patients serum reacting with the corresponding antigen on donor cell
3. An autoantibodies in patients serum reacting with the corresponding antigen on donor cell
4. Prior coating of the donor cell with proteins , resulting in positive AGT
5. Abnormalities in patients serum
6. Contaminants in the test system

4

A method for major crossmatch test type and screen coupled with and immediate crossmatch

Abbreviated crossmatch

5

Name the 4 methods in major crossmatching

Immediate spin crossmatch
Abbreviated crossmatch
Electronic/computer crossmatch
Antiglobulin crossmatch

6

A method for major crossmatch test that is accomplished by simply mixing a patient serum with donor cells and centrifuging immediately.

Immediate spin crossmatch

7

A method for major crossmatch test that compares recent ABO serologic results and interpretation on file for both the donor and the patient being matched and determine compatibility based on this comparison.

Electronic crossmatch

8

This procedure begins at the same manner as the immediate spin crossmatch continues at 37*C incubation and finishes with AGT

Antihuman crossmatch

9

In immediate spin crossmatch absence of hemolysis agglutination indicates?

Compatibility

10

Compatibility testing protocol

Testing of donor sample
Testing of patient sample

11

Compatibility testing consist of the ff.

Review of patients past blood bank history and records.
ABO and RH grouping of the recipient and donor.
Antibody screening of the recipient and donor's serum.

12

Used to detect antibodies bound to erythrocyte in vivo.

Direct globulin test

13

Used to detect the reaction of the antibodies and erythrocyte in vitro after an appropriate incubation phase

Indirect globulin test

14

What are the application of direct anti globulin test?

HDN
HTR
Autoimmune and drug induced hemolytic anemia

15

AGT is commonly called?

Coomb's test

16

What are the factors affecting anti globulin test?

Ratio of serum to cell
Reaction medium
Temperature
Incubation time
Washing of cells
Saline for washing
Addition of anti human globulin
Centrifugation for reading

17

Consist of mixing patient serum to donor cell

Major cross match