Control of Gene Expression

Question 1

Gene regulation requires (2)

Answer 1

1) short stretches of DNA defined sequences which serve as recognition sites for DNA binding proteins
2) Gene regulatory proteins which are transcriptional factors that will bind and activate genes

Question 2

The recognition sequence for regulatory proteins is ____ and can be proximal or distal to the first ____

Answer 2

TGATAG

Exon

Question 3

There are 4 possible configurations of DNA for regulatory protein to bind to such as

Answer 3

Hydrogen Bond Donors

Hydrogen Bond Acceptors

Methyl Groups

Hydrogen Atoms

Question 4

Every transcription factor WILL and MUST have (2)

Answer 4

a DNA binding module and an activation module

Question 5

Every transcription factor COULD have (2)

Answer 5

dimerization and regulatory module

Question 6

Abnormality in which gene causes Hereditary Spherocytosis?

Answer 6

KDL1 causing change in A.A sequence to RDR from RER leading to less RNA formation

A zinc finger protein

Question 7

Helix-Turn-Helix

Answer 7

Simples most common DNA binding Motif

Two alpha helices connected by SHORT chain of A.A

Long helix is the recog. site for dna binding module

The side chains of A.A recognize DNA motif

Question 8

Zinc Finger Domain

Answer 8

Has a zn atom
Binds to major groove
Found in tandem clusters**
Multiple contact points

Question 9

Leucine Zipper Motif

Answer 9

Two alpha helical DNA binding domain

Grabs DNA like clothespin

Activation domain overlaps with dimer domain

Dimerization via leucine zipper regions

Leucine residue every 7 A.A

Question 10

HELIX-LOOP-HELIX

Answer 10

Short alpha chain connected by a loop to a longer alpha chain

Can be homo or heterodimers

Question 11

How can gene activator proteins modify DNA (4)?

Answer 11

Nucleosome remodeling (nucleosome sliding)

Nucleosome Removal

Histone Replacement

Histone Modification