CORE PRACTICAL 11

Question 1

What is core practical 11? (1)

Answer 1

Investigate the presence of different chloroplast pigments using chromatography

Question 2

What is the first stage of this practical? (1)

Answer 2

Cut spinach leaves into small pieces with scissors and place into a mortar with a sprinkling of sand.

Question 3

What is the second stage of this practical? (1)

Answer 3

Use a pestle to grind the mixture, add a small amount of propanone to the paste as you grind.

Question 4

What is the third stage of this practical? (1)

Answer 4

Allow the mixture to settle and draw up the dark green chlorophyll solution with a small pipette, place this in a small, sealed tube and allow any solids to settle. The mixture can be filtered but you need to add more propane as some is absorbed by the filter paper.

Question 5

What is the fourth stage of this practical? (1)

Answer 5

To prepare a chromatogram, cut a piece of chromatography paper to fit the container, use a pencil to draw a line 1cm above the bottom