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Flashcards in Cytology Lab 1 Deck (76):
1

The two most common LM stains are

1.) Hematoxalyn (blue)
2.) Eosin (Red)

2

What had a thinner section, LM or EM?

EM

3

What is the theoretical limit of resolution for LM?

0.20 um

4

What is the theoretical limit of resolution for EM?

0.2-0.5 nm

5

A barrier between two aqueous compartments made up of choesterol, glycolipids, phospholipids, and proteins

Plasma membrane

6

Associated with nuclear lamins

Inner nuclear membrane

7

Continuous with endoplasmic reticulum. Can not distinguish a double membrane in LM

Outer nuclear membrane

8

We can not see mitochondria under LM using an

H and E stain

9

The golgi will appear with what type of stain?

Silver stain

10

With an H and E stain, the golgi is?

Unstained (will be a white region near nucleus)

11

Structures in the cytoplasm that are formed from the metbolic products of the cell

-non-living components of the cell

Inclusions (Glycogen, Lipids, Pigment granules)

12

The only inclusion with a membrane is the

Pigment granules

13

Membrane bound packets of stored products which have gone through the biosynthetic pathway

Secretory Granules

14

Cells storing many granules are indicative of

Regulated secretion

15

Light vacuolated appearance is indicative of

SER and Lipid Droplets

16

We can not see the cytoskeleton under

LM

17

Form the structural basis of microvilli, sterocilia, and adherent junctions

Actin

18

Involved in the intracellular trafficking of vesicles

Microtubules

19

Provide mechanical support for the cell

Intermediate filaments

20

Under LM, the RER stains

Blue

21

Lines all surfaces (external and internal) is avascular, and is highly cellular

Epithelium

22

Forms the parenchyma (secretory portion) of
exocrine and endocrine glands

Epithelium

23

Functions in protection and regulation of water and nutrient uptake

Apical plasma membrane

24

Which part of the epithelium provides protection to underlying connective tissue?

Skin

25

If the epithelium has one layer it is classified as

Simple

26

If the epithelium has two or more layers it is classified as

Stratified

27

Stratified in appearance, however, surface cells can be dome shaped or flat and the nuclei are ROUNDED in appearance

Transitional epithelium

28

All cells rest on the basement membrane, but not all cells reach the luminal surface

-Cell surface modifications (Cilia and Stereocilia) are ALWAYS present

Pseudostratified columnar epithelium

29

If present, should be included in the classification of
epithelial tissue.

Surface modifications and Goblet cells

30

Supports the tissue and forms the boundary between epithelium and connective tissue

Basement membrane or Basal Lamina

31

Line blood vessels and ventricles and atria of the heart

Simple Squamous epithelium

32

Simple squamous epithelium function in

Fluid transport, gas exchange, and lubrication

33

Located mainly in ducts of glands, covering of ovaries, and kidney tubules. Functions in secretion, absorption, and protection

Simple Cuboidal Epithelium

34

Wine glass shaped cell filled with mucus droplets (glycoprotein) which are secreted and function to lubricate luminal surface

Goblet Cell

35

Stratified epithelium is identified by the layer that is

Closest to surface of lumen

36

We NEVER find cilia or sterocilia at the cell surface of

Stratified epithelium

37

In keratinized stratified squamous epithelium, the cell layer closest to the lumen lack

Nuclei

38

Located in the ducts of sweat glands

Stratified cuboidal epithelium

39

Will ALWAYS have a surface modification such as cilia or sterocilia

Pseudostratified Epithelium

40

Do pseudostratified squamous and pseudostratified cuboidal epithelium exist?

No

41

Invaginations of the plasma membrane in transitional epithelium that play an important role in the permeability barrier

Unit Membrane (UM)

42

Maintains selective passage of substances from one side of the epithelium to the other

Tight Junction

43

Secretes its product via ducts to the surface

Exocrine Glands

44

Secretes product directly into blood stream via fenestrated capillaries and lymphatic vessels

Endocrine glands

45

Composed of relatively few cells and a relatively large amount of intercellular material

Connective tissue (Stroma)

46

The ECM is made up of which two components?

Fibers and ground substance

47

Produces both the fibers and the ground substance

-Principal cell of connective tissue

Fibroblast

48

Mostly collagen with just a little elastic tissue

-Connect muscle to bone

Tendons

49

Collagen, but with more elastic fibers since we want it to stretch and return to original shape

-Connects bone to bone

Ligaments

50

Coarser collagen bundles are used for

Tendons and Ligaments

51

Surround individual muscle cells, nerve fibers, and supporting lymphoid tissues

Fine Reticular Fibers

52

Fibers are less common and a highly hydrated ground substance predominates

Loose connective tissue

53

The polyanionic properties of ground substance enable it to hold Na+ ions and water in the connective tissue and thus

Resist compression

54

Have a significant influence on the passage of materials across connective tissue

Glycosaminoglycans

55

Provides storage of substances (mostly triglycerides) that can be metabolized to produce energy

White adipose tissue

56

Functions in thermogenesis

Brown adipose tissue

57

White blood cells are found in

Connective tissue

58

Have a great capacity to proliferate in response to
injury. Thus connective tissues usually heal well after injury

Fibroblasts and Mesenchymal cells

59

The dead components of connective tissues are degraded by

Macrophages

60

What are the three types of cells that reside in CT?

Fibroblasts, adipocytes, WBCs, and mesenchymal cells

61

Has a well developd RER and golgi and a long, tapered nucleus

-secretes ECM

Fibroblast

62

Undifferentiated cells that retain the potential to develop into fibroblasts

Mesenchymal cells

63

Found scattered throughout loose CT

-not membrane bound

Adipocyte

64

Has darker pink staining due to increased collagen bundles

Dense CT

65

All collagen bundles run in the same direction in

Dense Regular CT

66

Collagen bundles run in all different directions in

Dense Irregular CT

67

Easy to distinguish because they contain numerous secretory granules

Mast cells

68

Usually a small cell, mostly nucleus with a rim of cytoplasm

-large collections found in lamina propria

Lymphocyte

69

Have extensive RERs and their nuclei tend to be shifted towards one side

Plasma Cells

70

Tendons and Ligaments are made up of which type of CT?

Dense regular

71

The dermis of the skin is made up of which type of CT?

Dense irregular

72

Reticular fibers (type III collagen) are found in

Lymph nodes

73

Macrophages contain many

Lysosomes

74

Contains numerous fat droplets of varying sizes

-Nucleus is rounded

Brown fat

75

A specialized white blood cell that leaves the circulation to function in phagocytosis and respond to parasitic infections

Eosinophils

76

Most common monocyte

-Functions in phagocytosis

Neutrophil

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