Day 9.3 Biochem Flashcards Preview

* DIT each session > Day 9.3 Biochem > Flashcards

Flashcards in Day 9.3 Biochem Deck (162):
1

Organizational relationship bt DNA and chromosomes

DNA --> nucleosomes --> chromatin --> chromosomes

2

What is a nucleosome?

Group of 8 histones w DNA wrapped around twice.
2 each of H2A, H2B, H3, H4

3

What AAs make up the histone octamer

Mostly lysine and arginine, which are positively charged, and therefore ionically bind the negatively charged DNA

4

Which histone is not part of the histone octamer (the nucleosome core)?

H1

5

When does DNA condense?

In mitosis, it condenses to form mitotic chromosomes.

6

Heterochromatin, Euchromatin

HeteroChromatin = Highly Condensed.
Transcriptionally inactive, sterically inaccessible.

Euchromatin (Eu = True-ly transcribed)
Less condensed, transcriptionally active and sterically accessible.

7

How do additions to lysine affect DNA transcription?

Adding an acetyl or removing a methyl group decreases DNA affinity for the histone, causing it to become euchromatin.
Euchromatin = transcriptionally active.

So, if you add acetyl or remv methyl, you can make proteins!

If you do the opposite- remove acetyl or add methyl, you make the DNA have MORE affinity for the histone --> heterochromatin.

8

Methylation of DNA strands during replication

On the template (parent) strand, cytosine and adenine are methylated during DNA replication.

This allows mismatch repair enz to distinguish b/t the old and new strands- it's more likely that the new strand is going to be the one with the problem.

9

What effect does hypermethylation have on the transcription of DNA

Adding methyl groups causes DNA to become condensed into Heterochromatin, so transcription can't occur.
Hypermethylation inactivates DNA transcription.

10

What effect does histone acetylation have on DNA transcription?

Histone acetylation relaxes DNA coiling (changes it to euchromatin), which allows transcription to take place.

11

Methylation effect on DNA
Acetylation effect on DNA

Methylation makes DNA Mute (heterochromatin, highly condensed/transcriptionally inactive form)
Methyl groups are sml, so they allow histones to stay together.

Acetylation makes DNA Active
(euchromatin, transcriptionally active form)
Acetyl groups are bigger, so they push DNA away from histones.

12

What are the nucleotides? How many rings? What's in DNA vs RNA

Purines: A, G; 2 rings

Pyrimidines: C, T, U (1 ring- Py (pies) have a single ring)

DNA = A, G, C, T
RNA = A, G, C, U

13

Which nucleotide has a ketone?
Which has a methyl?

Guanine has a ketone

Thymine has a meTHYl

14

How is uracil made?

Deamination of cytosine.
The NH2 is taken off of cytosine and replaced w an O.

15

Number of bonds between the nucleotides

G-C has 3 H+ bonds
A-T has 2 H+ bonds

So, DNA with higher G-C content is harder to melt, therefore has a HIGHER melting temp.
(Note that DNA length also affects melting temp- longer DNA has higher)

16

Difference bt nucleotide and nucleoside

Nucleoside = Sugar(ribose) + Base
Nucleotide = Sugar(ribose) + Base + Phosphate(mono/di/tri)

Nucleotides are linked by 3' to 5' phosphodiester bonds.

17

Adenine vs adenosine
Guanine vs guanosine

the -sine is the nucleoside (Base + sugar).
otherwise, it just means the base.
So adenine and guanine are bases.
Adenosine and Guanosine are the nucleosides (base + sugar).

18

What AAs are necessary for purine synthesis? What are the carbon sources?

Glycine
Aspartate
Glutamate

CO2, glycine, and THF are the sources of carbons.

19

What is necessary for Pyrimidine synthesis?

Aspartate and Carbamoyl phosphate
The precursors for carbamoyl phosphate are CO2 (for the carbon) and glutamine (for the nitrogen)
Also need THF to make it.

20

Is glycine needed in purine or pyrimidine synthesis?

Only purine synthesis

21

What are the precursors for purines? pyrimidines?

Purines: IMP precursor
IMP --> AMP
IMP --> GMP (this is by IMP dehydrogenase)

Pyrimidines: Orotate precursor, PRPP is added later.
Orotic acid --> OMP --> UMP --> UDP
UDP --> > > > dTMP (DNA) (this is by thymidylate synthase)
UDP --> CTP

22

Which is made first, ribonucleotides or deoxyribonucleotides?

Ribonucleotides.
The deoxyribonucleotides come from the ribonucleotides. Ribonucleotide reductase is the enz that converts them.

23

What 2 pathways use carbamoyl phosphate?

Denovo pyrimidine synthesis
Urea cycle

24

OTC deficiency

Ornithine transcarbamoylase (OTC) normally converts carbamoyl phosphate to Citurulline, which is part of the urea cycle.

In OTC deficiency, the carbamoyl phosphate cannot be converted to citrulline and builds up, so it enters the other pathway it's part of- denovo pyrimidine synthesis. Carbamoyl phosphate is converted to orotic acid.

25

How is carbamoyl phosphate made?

ATP + CO2 + Glutamine
and the enzyme CPS-2

26

What is the rate-limiting step in pyrimidine synthesis?

CPS-2 enz, which converts ATP, CO2, and glutamate into carbamoyl phosphate.
(Carbamoyl phosphate is then converted to orotic acid and pyrimidines are made from that.)

27

How does the order differ in pyrimidine vs purine synthesis?

Purines:
1. Start w Sugar and PRPP (phosphate)
2. Add base

Pyrimidines
1. Start w (temporary) base (Orotic acid)
2. Add Sugar and PRPP (phosphate)
3. Modify the base

When you build a pyramid, you need to start w a base!

28

What are the steps in pyrimidine synthesis?

Orotic acid + PRPP (+orotic acid phosphoribosyltransferase)
OMP (+5'-phosphate decarboxylase)
UMP
UDP (+ribonucleotide reductase)

UDP --> CTP
or
UDP --> dUDP --> dUMP (+THF and thymidylate synthase) --> dTMP

29

What role does folate play in pyrimidine synthesis?

Methylene THF is needed to convert dUMP to dTMP (final pyrimidine product), along with the enz thymidylate synthase.

In this process, methylene THF is converted to DHF.

DHF is converted back to THF by the enz dihydrofolate reductase. (And the THF is converted to methylene THF)

30

What are the steps in purine synthesis?

Ribose-5-P (+PRPP synthetase)
PRPP (+gluamine PRPP amidotransferase)
>>+Hypoxanthine -->>>>
IMP

IMP --> AMP
or
IMP (+IMP dyhydrogenase) --> GMP

31

What is the rate-limiting step of purine synthesis?

Glutamine PRPP amidotransferase, which converts PRPP to the next step in the pathway to make IMP.

32

What does ribonucleotide reductase do? What drug blocks it?

RNR converts UDP --> > > dTMP

(It also makes deoxy-nucleotides from the other ribonucleotides)

Blocked by Hydroxyurea
(anti-cancer, used in sickle cell)

33

What does PRPP synthetase do? What drug blocks it?

PRPP synthetase converts Ribose-5-P to PRPP in the first step of denovo purine synthesis.

Blocked by 6-MP (mercaptopurine)

34

What does Thymidylate Synthase do?
What drug blocks it?

Converts dUMP to dTMP (folate is also req'd)

Blocked by 5-FU (anti-cancer)

35

What does IMP dehydrogenase do?
What drug blocks it?

Converts the IMP to GMP in purine synthesis.
Blocked by Mycophenylate (an immunosuppressant)

36

What does Dihydrofolate Reductase do?
What drugs block it?

DHFR converts DHF to THF (need to regenerate the THF for pyrimidine synthesis)
MTX blocks it in eukaryotes
TMP blocks it in prokaryotes (so use for bacterial infections)

37

What causes orotic aciduria?

Can't convert Orotic Acid to UMP in the denovo pyrimidine synth pathway.

Caused by defect in one of two enz:
1. orotic acid phosphoribosyltransferase (which converts orotic acid to OMP)
2. orotidine 5'-phosphate decarboxylase
(which converts OMP to UMP)

Auto-recessive.

38

Findings in orotic aciduria

Since you can't convert orotic acid to UMP, orotic acid builds up, and there is increased orotic acid in urine.

There is also megaloblastic anemia (which, unlike most of the time, does NOT improve w admin of folate or B12)

And FTT.

No hyperammonia (vs OTC def- which is also increased orotic acid)

39

If there is increase orotic acid in the urine, what is the next thing you should check?

Ammonia levels.

Hyperammonia - OTC deficiency (bc can't get rid of it in the urea cycle)

No hyperammonia - orotic aciduria (urea cycle is fine)

40

Rx for orotic aciduria?

Oral uridine administration

Just bypass the pathway w the faulty enz and start pyrimidine synthesis w the UMP.

41

What is the goal of breaking down purines?

Turn them into uric acid, which can be excreted in urine.

42

What can Guanine be converted to?

GMP (guanylic acid)
Guanosine (nucleoside)
Xanthine

43

What can Hypoxanthine be converted to?

IMP (Inosinic acid)
Inosine
Xanthine

44

What can Adenine be converted to?

AMP (adenylic acid) only.
Converted by enz APRT (the equivalent of HGPRT, which converts Hypoxanthine and Guanine)

45

What is IMP converted to?

AMP or GMP

46

What two things can xanthine be made from?

Guanine or Hypoxanthine

47

What does xanthine oxidase do?

Converts Hypoxanthine to Xanthine, and also converts Xanthine to Uric Acid (which is excreted in urine)

48

What drug blocks xanthine oxidase?

Allopurinol. Since it doesn't allow uric acid to be formed, it is good for treating (chronic) gout.
Can also be used for the gout and hyperuricemia of Lesch-Nyhan syndrome

49

What is Lesch-Nyhan syndrome?

Defective purine salvage d/t absence of HGPRT (which converts hypoxanthine to IMP and guanine to GMP).
Buildup of guanine and hypoxanthine mean that they are both converted to xanthine, which makes a lot of uric acid.

Findings: Hyperuricemia, gout, MR, self-mutilation (esp lip-biting), choreoathetosis, aggression.

X-linked Recessive

HGPRT = He's got purine recovery trouble

50

What does HGPRT do?

H = hypoxanthine
G = guanine
Converts hypoxanthine and guanine to IMP and GMP respectively.

51

What is the treatment for Lesch-Nyhan

Allopurinol, which inhibits xanthine oxidase, so there is less uric acid in the blood.
Only fixes the gout and hyperuricemia tho, not the MR, CNS issues.

52

What drugs are metabolized by xanthine oxidase, and therefore will have increased toxicity if the pt is also taking allopurinol?

6-MP (anti-cancer), and the related drug Azithoprine.
These are both metabolized by xanthine oxidase.
Allopurinol inhibits xanthine oxidase, so XO won't be able to metabolize 6-MP as usual, so there will be more toxicity from 6-MP since it doesn't get metabolized.
(Toxic to bone marrow, GI, liver)

53

What is adenosine converted to?

AMP
or Inosine
(and inosine is then converted to hypoxanthine)

54

What enz converts adenosine to inosine?

Adenosine Deaminase (ADA)

55

What is adenosine deaminase (ADA) deficiency?

Can't convert adenosine to inosine.
So excess adenosine (and therefore excess AMP)

Excess ATP and dATP cause imbalance in the nucleotide pool via feedback inhibition of ribonuceloside reductase (RNR converts ribo to deoxyrib)

This prevents DNA synth and therefore decreases lymphocyte count (a lot).
Mjr cause of SCID

56

Main sx of SCID, cause

SCID- severe combined immunodeficiency.
Severe recurrent infections
Chronic diarrhea
FTT
No thymic shadow on xray

1st dz to be treated by experimental human gene therapy.

Can be caused by 7 different genetic problems, one of which is Adenosine Deaminase (ADA) deficiency.

57

Silent mutation

Can of one base pair (often in 3rd wobble position) that results in the same AA being created.
Since AA is the same, not very problematic.

58

Missense mutation

Mutation in a base pair that results in a different AA- but one that is similar to the structure of the original AA (aka it's a conservative mutation)
Not very problematic, unless the mutation is at an active site- then can make the active site unable to bind/non-fnl.

59

Nonsense mutation

Stop the nonsense!
Base pair change that results in a stop codon being produced instead of the normal AA.

60

What are the 3 stop codons?

UGA
UAG
UAA

61

Frameshift mutation

Change (insertion or deletion) of a base pair such that the 3-base pair frame is shifted, so all AAs downstream of the shift are misread.
Usu results in truncated, non-fnl protein

62

Rank the severity of damage of DNA mutations

1. Nonsense (worst)
2. Missense
3. Silent

63

Origin of DNA replication

Particular seq in genome where the DNA replication begins
Prokaryotes: 1 origin
Eukaryotes: multiple origins

64

Replication fork

Y-shaped region along DNA template where the leading and lagging strands are synthesized.

65

Helicase

Unwinds DNA template at the replication fork.

If I were an enz I would be helicase... ;)

66

SSB proteins

Single-stranded binding proteins
Hold the two DNA strands apart at the fork to prevent them from re-annealing

67

DNA Topoisomerase II

aka DNA gyrase
Creates a nick in the DNA to relieve the supercoils created during replication by helicase.

68

What abx inhibit DNA gyrase?

(inhibit prokaryotic topoisomerase II):
Flouroquinolones
Inhibition means the bacteria are unable to replicate and divide.

69

Primase

Makes the RNA primer on which DNA polymerase III will initiate replication

70

DNA Polymerase III

Prokaryotic only.
Starts at RNA primer put down by primase.
Elongates leading strand by adding deoxynucelotides to the 3' end.
Elongates lagging strand until it reaches the primer of the preceding fragment.
3' --> 5' exonuclease acitivity "proofreads" each added nucleotide. (But synthesis is 5' --> 3' as always)

71

DNA Polymerase I

Prokaryotic only.
Excises the RNA primer w a 5' --> 3' exonuclease.
Degrades the RNA primer and fills in the gap w DNA

72

DNA ligase

Seals strands together after DNA Polym I has removed the RNA primer and filled in the gaps.

73

What are the DNA polymerases for eukaryotes (and what do they do)?

alpha: synth's RNA primer and replicates lagging strand
beta, epsilon: repair DNA
gamma: replicated mito DNA
delta: replicates leading strand

74

What are the anti- topoisomerase Ab, and what dz are they assoc w?

Anti- Scl-70
A/w diffuse scleroderma

75

What cancer drug inhibits the eukaryotic topoisomerases?

Etopiside

(eTOPiside inhibits TOPoisomerases)

76

Telomerase

Adds DNA to the 3' ends of the chromosome to avoid loss of genetic material every time there is replication.
TTAGGG is the seq added.

In adults, this only occurs in stem cells and immune cells (things that need to divide a lot)

Also occurs in cancer- imp step for neoplastic transformation.

77

Enz w 5' --> 3' exonuclease activity

DNA polymerase I
Excises the RNA primer
(then degrades it and fills in gap w DNA)

78

Enz w 3' --> 5' exonuclease activity

DNA polymerase III
Adds nucleotides, elongating the leading and lagging strands. The exonuclease "proofreads" each added nucleotide.

Note: even tho the exonuclease is backwards, synthesis (as always) goes in the 5' --> 3' direction.

79

What are the three methods of single strand DNA repair?

Nucleotide excision repair
Base excision repair
Mismatch repair

(for dbl strand repair, there is non-homologous end-joining)

80

Nucleotide excision repair

Specific endonucleases rls the oligonucleotide-containing bases that are dmgd. This is for a small segment of DNA (vs base excision, which is just for a base)
DNA polymerase fills in the gap and ligase reseals it.

81

Base excision repair

Specific glycolases recognize and remv the dmgd base, AP endonuclease cuts DNA at the apyrimidinic site, empty sugar is remvd, and the gap is filled in and resealed (polymerase, ligase)
This is for one base (vs nucleotide excision, which is for a segment)
Imp in repair of spontaneous/toxic deamination.

82

Mismatch repair

When DNA is made, the old strand has methyl groups, but the new strand has not be methylated yet- so this is how it's recognized as new.
The unmethylated, new DNA is recognized, and mismatched nucleotides are removed. Then the gap is filled and sealed.

83

What are dz's caused by defects in DNA repair?

Xeroderma pigmentosa (nucleoside excision repair defect)
Bloom's syndrome
HNPCC Hereditary NonPolyposis Colorectal Cancer (mismatch repair defect)
BRCA1 and BRCA2
Ataxia-Telangiectasia (Non-homologous end joining defect)

84

What is the DNA repair mechanism that deals with double-stranded repair? How dies it work?

Non-homologous end-joining.
Brings together 2 ends of DNA fragments
No reqmt for homology
Caused by x-ray, CT radiation

85

Xeroderma pigmentosum

Dry skin w melanoma and other skin cancer, children of the night.

D/t nucleotide excision repair defect, which prevents the repair of thymidine dimers (which are caused by exposure to UV)

86

Ataxia-Telangiectasia

Triad of:
Cerebellar defects (ataxia)
Spider angiomas (telangiectasia)
IgA deficiency (so immunodeficient)

Caused by defect in non-homologous end joining (DNA repair)
Insensitivity to ionizing radiation

Gait ataxia starts at 1-2 years, need to dx right away, before the telangiectasias (which happen at 5yo).
No smooth pursuit, aFP may be elevated (after 8mo old)

87

Bloom's syndrome

HPS to sunlight
Leukemias and lymphomas common
Avg age of cancer onset is 25
d/t DNA repair defect

88

HNPCC

Hereditary NonPolyposis Colorectal Cancer

Caused by auto-dom mutation of DNA mismatch repair genes.

80% progress to colorectal cancer
proximal colon always involved.

89

BRCA1 and BRCA2

Tumor suppressor genes
Loss of fn --> cancer (and BOTH alleles must be lost)

Normal gene product is DNA repair protein, so if mutated, there will be defective DNA repair.

BRCA1 causes breast and ovarian cancer
BRCA2 causes breast cancer

90

What direction are DNA, RNA, and proteins read and made?

5' --> 3' direction
mRNA is read 5' --> 3'
Protein synth is N to C

The incoming nucleotide (the one being added) has the triphosphate on it, which is the energy source for the bond that's about to be made.

The triphosphate bond is attacked by the 3'OH on the existing strand.

91

Drugs that block DNA replication (HIV, chemo) often have what biochemical modification to their DNA?

the 3'OH on the existing DNA strand is modified, meaning that the 3'OH can't attach the triphosphate of an incoming nucleotide in order to add it to the chain (aka chain termination happens, bc nothing more can be added)

92

What are the types of RNA?

rRNA (most abundant)
mRNA (longest)
tRNA (smallest)

RNA is rampant, massive, tiny
rRNA has to be the most abundant, bc it makes up the ribosomes!
mRNA has to be the longest, bc some genes are super long

93

Start codon, and what it codes for (which AA)

AUG
(AUG inAUGurates protein synthesis)
Rarely, GUG can also start things.

Eukaryotes: AUG codes for methionine
(the methionine may be removed before the translation is completed tho, making it look like it started w something else!)

Prokaryotes: AUG codes for formyl-methionine (f-Met)

94

What are the stop codons?

UGA U Go Away
UAG U Are Gone
UAA U Are Away

These encode for releasing factors.

95

What is an operon

the genes that are transcribed (the coding region) plus everything that comes before it that makes transcription happen- the promotor region, regulatory regions, operator region, enhancer region, etc

96

What are transcription factors?

Proteins that must bind to the promotor region in order for transcription to take place

97

What are the 4 common structural motifs of transcription factors?

DNA is hard to bind to, so the transcription factors have to have special shapes:
Helix-Loop-Helix
Helix-Turn-Helix
Zinc Finger
Leucine Zipper Protein

98

What are the promotor regions?

Places where the RNA polymerase and the transcription factors bind and therefore initiate transcription. Rich in A and T.

There are 2:
-25 TATA box (aka Pribnow, Hogness)
it's 25 nucleotides before the transcription initation site (start codon)

-75 CAAT box
it's 75 nucleotides before

99

What is the operator region? Where is it?

Located between the -25 promotor and the start site
It's a place that either binds a repressor (which stops transcription) or binds an inducer (starts transcription).

100

What are response elements?

Enhancer regions and repressor (aka silencer) regions- can increase the rate of transcription when bound by protein factors.
can be located close to, far from, or even within the promotor region

101

What is lactose broken down into? And what enz breaks it down?

Lactose is broken down into Glucose and Galactose.
Enz is B-galactosidase

102

What product does the lac operon make?

It makes B-galactosidase (when necessary). This is the enz that breaks down lactose into glucose and galactose.

103

When would you want the lac operon ON?

When you want to make B-galactosidase.
That is, when you want to make it bc a) you have no glucose and need to break down some lactose to get some
AND
b) lactose is there in the first place, waiting to be broken down

104

When would you want the lac operon OFF?

If you don't need B-galactosidase.
That is, if you already have glucose (so no point in breaking down lactose to get some)
and/or
if you don't have any lactose (no point in making the enz if it can't actually do anything)

105

Lac operon: when is CAP present? What does it do?

When there's no glucose, CAP is there.
CAP wants to help- it's pro- making B-galactosidase. It says hey! we need glucose!
(But, if a repressor is there, it can't do anything)

Glucose inhibits the CAP.

106

Lac operon: when is the lac repressor present? What does it do?

Repressor is there when there is no lactose. If there's no lactose, there's no point in making the enz that breaks down lactose.

When the lac repressor is bound, the RNA polymerase that makes the DNA can't bind.

When lactose is there tho, it binds the repressor, so the repressor can't bind and repress.

107

Lac operon analogy

Husband - glucose
Lover- CAP
Kid - repressor
Lactose - school

When the husband (glucose) is present, the lover CAP will not be there. When the husband is absent, the lover will be there.

When there is lactose school, the kid repressor will not be there. But, when there's no lactose school, the kid will be home.

So, in order for the CAP lover to come over, there needs to be no glucose husband, and there also needs to be lactose, so that the repressor kid will be away at school.

When the glucose husband is gone, the CAP lover is present, and the kid repressor is away at lactose school, it's ON!

108

What happens if there is a mutation in a gene's promotor region?

(Promotor regions = -25TATA and -75CAAT)
Mutation results in a dramatic decrease in the amt of gene transcribed (so much less protein is made)

109

DNA polymerases vs RNA polymerases in eukaryotes vs prokaryotes.

DNA polymerases make DNA
RNA polymerases make RNA

In prokaryotes, there is DNA polymerase III (which elongates the strands) and DNA polymerase I (which excises the RNA primer, degrades it, and fills in the gap with DNA)

In eukaryotes, there are DNA polymerases alpha, beta, gamma, delta, epsilon.

RNA:
In prokaryotes, there is only 1 RNA polymerase (a multisubunit complex) that makes all 3 kinds of RNA

In eukaryotes, there is RNA polymerase I, II, III (for rRNA, mRNA, tRNA respectively)

110

What does RNA polymerase I do?

RNA polymerases make RNA.
RNA polym I makes rRNA in eukaryotes

111

What does RNA polymerase II do?

RNA polymerases make RNA
RNA polym II makes mRNA in eukaryotes
It also opens DNA at the promotor site

112

What does RNA polymerase III do?

RNA polymerases make RNA
RNA polymerase III makes tRNA in eukaryotes

113

What makes RNA in prokaryotes?

An RNA polymerase- it is a multisubunit complex that makes all 3 kinds.

114

T/F RNA polymerases have proofreading function

False
No proofreading fn, but they can initiate chains.

115

a-amantin

Found in death cap mushrooms and inhibits RNA polymerase II
Causes liver failure if eaten.

116

Abx that inhibits prokaryotic RNA polymerase

Rifampin

117

RNA processing in eukaryotes

Occurs in nucleus, after transcription:
1. 5' cap (7-methylguanosine)
2. 3' tail, aka polyadenylation (~200 As) AAUAAA is the polyadenylation signal. The poly-A polymerase does not req a template.
3. Introns are spliced out by the spliceosome

Only RNA that has been processed like this can go out of the nucleus.

118

Why don't mitochondrial genes have a 5' cap?

RNA processing (adding the 5' cap) occurs inside the nucleus. Mitochondrial genes are made in the cytoplasm.

119

What is the initial RNA transcript called before processing? What about after?

Before: hnRNA (heterogenous nuclear RNA)
After it has been capped and tailed and spliced, it is mRNA, and can go out of the nucleus.

120

Splicing of pre-mRNA (hnRNA)

Once of the necessary processings of RNA in eukaryotes.
1. Primary hnRNA transcript combines w snRNPs and other proteins to form the spliceosome
2. Lariat (loop) intermediate is made
3. Lariat is rlsd to remove the intron, the 2 exons are joined

121

How is pre-mRNA splicing affected in pts w lupus?

Lupus pts make Ab to the snRNPs of the spliceosome

122

Exons vs Introns

Introns are what gets spliced out.
Exons are what stays.

Exons EXit the nucleus (!! not the transcript), which means they are EXpressed.

Introns are INtervening seq's that stay IN the nucleus (so they are not expressed)

Exons contain the info that codes for protein. Introns are intervening, non-coding segmts of DNA

123

Alternative splicing

Taking out different introns (eg 2 introns flanking an exon taken out so the exon is taken out too; for a different protein the exon would have stayed)

Combos of different exons make different proteins for different tsu's.

Ex: B-thalassemia mutations

124

Where is rRNA sythesized? mRNA? tRNA?

rRNA is made in the nucleolus of the nucleus
mRNA and tRNA are made in the nucleoplasm of the nucleus

125

Structure of tRNA

75-90 nucleotides
Secondary structure
Cloverleaf form
Anticodon end is opposite the 3' aminoacyl end.

The 3' OH end has a CCA and a lot of modified bases. The AA gets covalently bound to the 3' end of the tRNA (knocks off the OH)
CCA = can carry amino acids

126

What enz adds the amino acid to the tRNA molecule?

Aminoacyl tRNA synthetase (using ATP) adds the AA to the 3'OH end of the tRNA.
This is called "charging" the tRNA

There is a different aminoacyl-tRNA for each AA!

The enz also looks at the AA before and after it binds- if it is incorrect, the bond is hydrolyzed.

127

What happens if a tRNA is mischarged?

It reads that codon that it should (bc the anti-codon part of the tRNA is fine) but since the wrong AA is attached to the 3' end of the tRNA, the wrong AA will be inserted into the sequence.

128

What abx prevent attachment of aminoacyl-tRNA?

Tetracyclines- they bind the 30S subunit.

129

Where is the anticodon?

It's part of the tRNA.
The codon is part of the mRNA.

anTI is for T-RNA

130

tRNA wobble

The base pairing only needs to be right for the first two nucleotide positions of an mRNA codon / tRNA anticodon, so codons that have a different 3rd wobble position can code for the same AA.
This is d/t the degeneracy of the code.
It's also why silent mutations are silent.

131

Where are ribosomes synthesized?

They are made in the nucleus (then txfrd to the cytoplasm in order to do their job)

132

Protein synthesis: initiation

Activated by GTP hydrolysis
Initiation factors (eIFs) help assemble the 40S ribosomal subunit w the initiator tRNA (the one that has the Met and the anti-codon to mRNA's AUG)

eIFs are rlsd once the mRNA and the (other) ribosomal subunit assemble with the complex.

133

Protein synth: elongation

Elongation has 3 steps:
1. Aminoacoly-tRNA (that's a tRNA w an AA attached to it) binds to the A site*
2. Ribosomal RNA aka Peptidyl transferase aka ribozyme catalyzes peptide bond formation bt the new AA (the one on the tRNA in the A site) and the existing AA chain (which is on the tRNA in the P site).
When the peptide bond is made, all of the AA chain is transfered and now hanging off of the tRNA in the A site.
(the tRNA in the P site now has nothing on it)
3. Translocation: the ribosome advances 3 nucleotides toward the 3' end of the mRNA- this moves the empty tRNA to the E site, and moves the tRNA will the AA chain on it (the "peptidyl RNA") to the P site, and frees up the A site for the incoming tRNA with one AA on it.

*except the very first one (Met)- it doesn't bind to the A site

134

What proteins are needed to help with translocation?

In prokaryotes: EF-G
In eukaryotes: EF-2

(Translocation is when the ribosome moves 3 nucleotide toward the end of the 3' mRNA, shifting what's in the A, P, and E sites)

135

Protein synthesis: termination

Completed protein is rlsd from the ribosome thru hydrolysis (req's one GTP), and dissociates

136

Ribosomal subunits in eukaryotes and prokaryotes

Eukaryotes: 40S + 60S = 80S (Even)
PrOkaryotes: 30S + 50S = 70S (Odd)

137

When are ATP and GTP each needed during the process of protein synthesis?

ATP = tRNA Activation (charging)- this is when the AA is attached to tRNA, it actually occurs before protein synth occurs

GTP- used for tRNA Gripping (initial assembly) and Going places (translocation step of elongation)

138

A site, P site, E site

A site = incoming AminoAcyl tRNA (a tRNA with an AA attached)

P site = accommodates growing Peptide
but note: the peptide chain is transfered to the tRNA that's in the A site when the peptide bond is made)

E site: holds the Empty tRNA before it Exits

139

What part of protein synthesis do aminoglycosides inhibit?

They bind to the 30S subunit (prokaryotes) and therefore inhibit formation of the initiation complex - so they inhibit initiation. They also cause misreading of the mRNA.

140

How does Chloramphenicol inhibit protein synthesis?

It inhibits the 50S peptidyl transferase
(so it inhibits the elongation step)

Streptogramins do the same thing.

141

How do macrolides and linezolid inhibit protein synthesis?

They bind the 23S (peptidyl transferase, ribozyme) part of the 50S subunit, and therefore block translocation.

142

How does Clindamycin inhibit protein synth?

Binds 50S, blocking translocation

143

How do tetracyclines inhibit protein synthesis?

Bind to 30S
They inhibit the binding of the Aminoacyl-tRNA to the A site.

144

Trimming (post-translational mods)

Removal of N-terminal or C-terminal propeptides from zymogens to create mature proteins

145

Covalent alterations post-translation

Phosphorylation
Glycosylation, Hydroxylation on collagen

146

Post-translational proteosomal degradation

Attachmt of ubiquitin to defective proteins to tag them for breakdown.
Ubiquitin protein ligase identifies the tagged proteins, and they are sent to the proteosome

147

3 ways to do proteolysis

Ubiquitination (sent to proteosome)
Lysosomal degradation
Ca2+-dependent enz degradation

148

Which parts of collagen synthesis happen inside fibroblasts? Outside?

Inside:
Sythesis (RER)
Hydroxylation (ER)
Glycosylation (ER)
Exocytosis

Outside:
Proteolytic processing
Cross-linking

149

Synthesis, hydroxylation, glycosylation, exocytosis of collagen

These steps happen inside the fibroblasts:
1. Synthesis (RER) - collagen alpha chains (preprocollagen) are translated in the RER. Usu makes Gly-X-Y (with X and Y being proline, hydroxproline, or hydroxylysine)

2. Hydroxylation (ER)- hydroxylation of specific proline and lysine residues.
This step req's Vit C! (if not, get scurvy)

3. Glycosylation (ER)- Glycosylation of pro-a-chain lysine residues and the formation of procollagen (triple helix of 3 a chains)

4. Exocytosis of procollagen into extracelluar space.

150

Which AAs of collagen get hydroxylated?

Proline and Lysine
This req's Vit C

151

Which AA of collagen gets glycosylated?

Lysine

152

What is scurvy?

Lack of Vit C, which means the proline and lysine of collagen can't be hydroxylated, so collagen synthesis is impaired.

Collagen synth defect causes swollen gums, bruising, anemia, poor wound healing, tongue petechiae

153

What steps of collagen processing occur after the exocytosis of collagen into the extracellular space (outside of fibroblasts)?

1. Proteolytic processing: cleavage of terminal regions of procollagen. This transforms it into insoluble tropocollagen.

2. Cross-linking: covalent crosslinking of lysine-hydroxlysine (by enz lysyl oxidase) reinforces the many staggered tropocollagen molecules and makes collagen fibrils.

154

What is Ehlers-Danlos?

Faulty collagen synthesis, causing hyperextensible skin, tendency to bleed (easy bruising) and hypermobile joints.

Type III collagen is most freq'ly affected.

Happens when collagen can't crosslink its lysine and hydoxylysine to make collagen fibrils (lysyl oxidase is the enz)

155

How is collagen synthesis affected in osteogenesis imperfecta?

OA is the inability to form pro-collagen from pro-a chains
Have the chains, but can't make the triple helix with them.

156

Drug that inhibits 50S peptidyltransferase

Chloramphenicol and Streptogramins

157

Drug that binds to 50S and blocks translocation

Macrolides and Linezolid

158

Drug that binds to 30S, preventing attachment of tRNA

Tetracyclines

159

Drug that inhibits prokaryotic DNA polymerase

Rifampin

160

Drug that inhibits prokaryotic topoisomerases

Fluroquinolones
(topois = gyrase)

161

Drug that inhibits prokaryotic DHF reductase

TMP trimethoprim

for eukaryotes- MTX methotrexate

162

Enz that matches AAs to tRNA

Aminoacyl tRNA synthetase
this is called "charging"