DNA Lab Quiz Flashcards

1
Q

definition for Nucleotide

A

monomer of DNA

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2
Q

What are the building blocks of nucleic acid?

A

Phosphate group
Deoxyribose (pentose sugar)
Nitrogen base (1 of 4)
 Adenine (A)
 Thymine (T)
 Cytosine (C)
 Guanine (G)

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3
Q

Name the nitrogenous bases that are classified as purines.

A

adenine and guanine

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4
Q

Name the nitrogenous bases that are classified as pyrimidines.

A

thymine and cytosine

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5
Q

What kind of bonds hold the nitrogenous bases together?
a. How many for GC and how many for AT?

A

h bonds
3 bonds for guanine and cytosine
2 bonds for adenine and thymine

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6
Q

Where is a phosphodiester bond located?

A

on 3 carbon

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7
Q

What is the difference between 5’3’ and 3’5’?

A

The numbers for the two sides are divide from the placement of the five carbon sugar in double helix. For visual purposes for the 53 strand by purely looking at it it goes 54321 and for the 35 strand it goes 12345 with it being turned upside down.

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8
Q

Who established the base pair rule?

a. What does the base pair rule state?

A

Erwin Chargaff
The amount of A, T, G, C varies from species to species
In each species
The amount of Adenine equals Thymine
The amount of Guanine equals Cytosine

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9
Q

Who developed the model of the DNA molecule?

a. In what year was it published?

A

Established by James Watson and
Francis Crick in 1953

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10
Q

In RNA, what nitrogenous base is substituted for Thymine?

A

uracil

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