doc Meme 1 Flashcards
1
Q
genogroup of CSFV in the country
A
G2, G3
2
Q
Indirect ELISA: test serum is placed in wells of _____
A
polystyrene microtitre plates
3
Q
is the antiglobulin linked to an enzyme
A
conjugate
4
Q
ELISA Color reaction is visualised and measured with
A
spectrophotometer
5
Q
antigen is pre-adsorbed at the bottom of the well T.F
A
true
6
Q
how many nm is used to determine adsorbance
A
405 nm
7
Q
used to
measure optical density (OD) or color intensity of
the reaction for each well
A
Spectrophotometer
8
Q
test validation:
A
neg control: <0.1
pos control: >0.1
9
Q
formula for S/P ration:
A
test sample OD - OD neg control/ OD pos control - OD neg control =
10
Q
S/P >0.25 =
S/P <0.25 =
A
pos
neg
11
Q
it is recommended to have no more than __ freeze-thaw cycle
A
3-5
12
Q
A
