qPCR Flashcards
(22 cards)
virions vs viruses
virion: infectious form of virus, extracellular, smaller, spheroidal in shape
virus: larger, intracellular, nucleoprotein particle
NA extraction steps
lysis
binding
washing
elution
after pipeting 20ul of master mix into pcr tubes, the ___, ___, and ___ will be added
negative control first
sample RNA second
positive control last
why is there a need to spin?
to remove bubbles
primer probe combination
PEDV RNA probe : ____
FAM fluorescence
TGEV RNA probe : ___
Quasar® 670/ Cy®5 fluorescence
Heterologous RNA control probe = ___
HEX fluorescence
PCR uses
medical research
diagnosis
contact tracing
forensics
drug devt
how to interpret CT with 4-cycle difference
the pink reaction has 16 times as much target sequence compared to red reaction
nuclease-free water why need?
nucleases have the tendency to degrade DNa KASI
For tissues, the volume of formalin used should be approximately __ times as that of the tissue.
10
formalin can be diluted using
dh20
A good transport medium for swabs for viral isolation is
NSS, PBS, 0.85% NaCl if possible with 1% gelatin
fecal samples should be chilled at __
4C
only sample that needed to be frozen
semen sample
use this when bacterial growth must be prevented
bacterial soln
refrigeration techniques
ice pack, dry ice
chemi preservation techniques
fixing solm
bactericidal soln
volt for electrophoresis
5-8V/cm
possible cause of false pos in downstream detection test
re-use of equipment
contaminated equipment
contaminated reagents
“ working area
possible causes of Failure in
downstream detection tests
bad sample
Damaged reagent bottles or spin columns
incorrect prep
incorrect sample vol
possible causes of no flow-through
blood clots
centrifuge force not enough
