RLG PARASITES

Question 1

pathogen Causing dermatologic lesions

Answer 1

Cercopithifilaria
dirofilaria repens

Question 2

Only sp. That detects antibodies

Answer 2

d. immitis

Question 3

Causes humpsores

Answer 3

stephanofilaria

Question 4

large sp of babesia

Answer 4

canis, vogeli

Question 5

vector of b. vogeli

Answer 5

r. sanguineus

Question 6

vector of b canis

Answer 6

dermacentor

Question 7

small sp. of babesia

Answer 7

b. gibsoni

Question 8

Causes neurologic signs
Adhere and clogs brain capillaries
Highly pathogenic

Answer 8

b. bovis

Question 9

present in ph

Answer 9

b vogeli
b. gibsoni
b. bovis
hepatozoon canis
theileria orientalis

Question 10

vector of t. evansi

Answer 10

tabanids

Question 11

TAE buffer function

Answer 11

used as a running buffer in gel electrophoresis to separate nucleic acids.

based on their molecular weight and net charge, the nucleic acids move either slowly or rapidly towards the positive pole. The smaller molecules run faster than the bigger molecules during the gel electrophoresis.

Question 12

other term for anaplasmosis

Answer 12

canine infectious cyclic thrombocytopenia

Question 13

LAMP temperature

Answer 13

60-65C

Question 14

sample that’s more useful when looking for babesia

Answer 14

capillary

Question 15

stain preferred for examination of protozoan parasites and rickettsiae

Answer 15

romanowsky - wright and giemsa

Question 16

how much blood is needed for blood smear

Answer 16

3-5 uL

Question 17

angle for blood smear

Answer 17

30-45C

Question 18

fixative for giemsa staining

Answer 18

methnaol

Question 19

knott’s concn technique

Answer 19

mix 1 ml uncoagulated blood with 9 ml 2% formalin

invert to mix 4x

centrifuge 500g x 5min (2000 rpm)

discard supernatant
staon sediment with 1-2 drops 1% meth blue

add drop of sample and cover

Question 20

No sheath; Body curved
with hooked posterior end

Answer 20

acanthocheilonema reconditum

Question 21

Sheathed; Tail is tapered
and with cephalic space.

Answer 21

brugia spp.

Question 22

size of brugia

Answer 22

170-230 um

Question 23

LAMP can amplify target DNA to what exponent

Answer 23

10 raised to 9

Question 24

LAMP utilizes a set of _- primers that recognize a total of six distinct sequences on the target DNA.

Answer 24

4-6

Question 25

meaning of CFI

Answer 25

color fluorescent indicator

Question 26

composition of CFI

Answer 26

hydroxynaphthol blue gel green

Question 27

LAMP fluorescence is confirmed via machine called

Answer 27

FAM filter

Question 28

advantages of microscopic exam

Answer 28

accessible can ID coinfection

Question 29

limitations of microscopic exam

Answer 29

low sensitivity difficult for species lvl ID

Question 30

advantages and disadvantages of serology

Answer 30

accessible, high sensitivity /// Abs take time to develop cannot distinguish past and present infection cross-reactivity

Question 31

molecular techniques adv and disadvantages

Answer 31

high sensitivity and specificity multiplex PCR // not readily avail expensive may take hours false negative cases

Question 32

LAMP meaning

Answer 32

loop-mediated isothermal amplification