Enzymes with temperature

Question 1

What happens to an enzyme and substrate solution when heated?

Answer 1

The enzymes and substrates molecules increase in kinetic energy, so they move faster, so more successful collisions so there are more enzyme - substrate molecules, increasing reaction rate

Question 2

What is an enzymes optimum temperature?

Answer 2

The temperature that enzymes and substrates make the fastest reaction rate

Question 3

What happens when an enzymes optimum temperature is surpassed?

Answer 3

The enzymes tertiary structure is broken and the active site not complementary to their substrate so the reaction rate decreases and the enzyme is denatured

Question 4

What bonds break when an enzymes optimum temperature is surpassed?

Answer 4

Hydrogen bonds

Ionic bonds

Question 5

What is a thermophilic?

Answer 5

Enzymes that work in high temperatures

Question 6

What is a psychrophilic?

Answer 6

Enzymes that work in low temperatures

Question 7

What is a temperature coefficient?

Answer 7

The increase to the rate of reaction when the temperature increases by 10 degrees

Question 8

What is the optimum temperature for animals?

Answer 8

40 degrees

Question 9

What is the equation of temperature coefficient?

Answer 9

rate of reaction(T of 10)degrees
Q10 = ——————————————–
rate of reaction 10 degrees

Question 10

What happens to the temperature coefficient after the enzymes optimum temperature is surpassed?

Answer 10

The temperature coefficient is decreases

Question 11

What is pH?

Answer 11

How acidic or alkaline a substance is

Question 12

What is an acidic pH?

Answer 12

0-6

Question 13

What is an neutral pH?

Answer 13

7

Question 14

What is an alkaline pH?

Answer 14

8-14

Question 15

What is an acid?

Answer 15

This disassociates into protons and negative ions

Question 16

What is a buffer?

Answer 16

They resit changes to pH by either donating or accepting H+ ions

Question 17

How does pH affect the enzymes intermolecular forces?

Answer 17

A hydrogen ion is attracted to the negative ions, molecules or parts of the molecules which replace the hydrogen bonds. The enzymes active site changes shape so it can’t take in its complementary substrate so the reaction rate decreases

Question 18

What happens to an enzyme if there is a small fluctuation in the pH?

Answer 18

The hydrogen bonds can be remade when it goes back to the optimum pH so the enzyme return back to normal so the active site is partially disrupted

Question 19

What happens to an enzyme if there is a large fluctuation in the pH?

Answer 19

The enzyme is fully denatured, and will be permanently damaged and can’t catalyse the reaction

Question 20

What is the optimum pH for amylase?

Answer 20

6.8

Question 21

What is the optimum pH for pepsin?

Answer 21

1 - 2

Question 22

What is the optimum pH for trypsin?

Answer 22

7.8