EX1 Opsonization and Phagocytosis - Quan Flashcards Preview

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Flashcards in EX1 Opsonization and Phagocytosis - Quan Deck (23):
1

The process of attaching opsonins, such as IgG or complement fragments, to microbial surfaces to target microbes for phagocytosis

opsonization

2

These are macromolecules attached to the surface of a microbe and can be recognized by receptors on neutrophils and macrophages

opsonins

3

Two primary types of opsonins are...

IgG and complement factor C3

4

Most microorganisms (will/will not) be phagocytosed (with/without) opsonins

will NOT
withOUT

5

Opsonins change the organisms surface form (hydrophilic/hydrophobic) to more (hydrophilic/hydrophobic)

hydrophillic
hydrophobic

6

Receptors for opsonins are…..

Fcγ on phagocytes
CR and leukocyte integrin Mac-1

7

The _____ region of IgG reacts with the organism

Fab

8

There are _____ classes of Fc (Fcγ) domains on PMNs

3

9

Fcγ RI (CD64) binds to ____ and _____ with (high/low/intermediate) affinity, it (is/is not) expressed on resting neutrophils, has ____ immunoglobulin-like binding domains, and expression is (up/down) regulated by IFN-γ, G-CSF, and infection

IgG1 and IgG3
high
is not
3
up regulated

10

Fcγ RII (CD32) binds to multiple IgGs with (high/low/intermediate) affinity, it is ______ spanning with a cytoplasmic tail, _____ different genes code for RII, ______'s RII exhibits genetically determined structural polymorphisms

low
membrane
3
neutrophils

11

Fcγ RIII (CD16) binds to _____ and _____ with (high/low/intermediate) affinity, ____ genes code of RIII
RIIIB is a molecule with a ________ anchor and is found only on ______
RIIIA has _______ and _____ domains and is found on ______ and _______

IgG1 and IgG3
intermediate
2
glycophosphatidylinositol
neutrophils
transmembrane and cytoplasmic
NK cells and macrophages

12

Fcγ R____ is the most important for phagocytosis of IgG coated microorganisms and is the sole class capable of binding _______ IgG2 complexes (IgG2 contains antibodies to bacterial capsular polysaccharides)

RII
human

13

Activation of the _______ cascade causes proteolytic cleavage of ________ factors creating potential _______ for receptors on _______ surfaces

complement
complement
ligands
neutrophil

14

These complement factors are chemotatic factors

C3a and C5a

15

These complement factors are the main complement derived opsonins

C3b and C3bi

16

CR1 (CD35) is a neutrophil complement ________, resides in ___ pools of receptors, it binds dimeric ______ with (strong/weak) binding

receptor
2
C3bi
weak

17

Complement R CR3 is a glycoprotein member of the ____ family, resides in ____ pools of receptors, and the whole molecule is termed the CD___ and CD___ complex

integrin
2
CD11 and CD18

18

_________ are the major phagocytic cells in the innate immune response, has _____ and ______ granules, and is (continually/periodically) circulating

neutrophils
primary and secondary
continually

19

________ circulate in the blood eventually moving into tissue where they differentiate into _________; of which ________ and produce _______

monocytes
macrophages
phagocytose
cytokines

20

4 factors thats can increase PMN numbers...

stress
injury
infection
increased cytokines

21

There are 2 primary mechanisms for destruction/killing of cells

oxidative and non-oxidative

22

The non-oxidative mechanism uses ______ and ______ granules and/or a ____ change

enzymes and cytoplasmic
pH

23

Name the 2 ways in which microbes can avoid phagocytosis

inhibiting/inactivating opsonization/phagocytosis
surviving intracellular killing