EXPERIMENT 2, 3, 4, 5, 6, 7 POST LAB Flashcards
used for the preservation of stained fecal smears
Polyvinyl alcohol (PVA)
preservation of trophozoites and cysts
Polyvinyl alcohol (PVA)
*Quality control: discard if cloudy
Polyvinyl alcohol (PVA)
For concentration techniques or direct fecal smear
5-10% Formalin
Not suffcient for preparing permanent stained feel scr
5-10% Formalin
*Quality control: Heat at 60°C before use
5-10% Formalin
For bulk feces, the solution is mixed in the proportion of 9.4 ml and 0.6 ml Lugol’s for a gram of fecal material
Merthlolate lodine Formalin (MIF) or Thimerosal lodine Formalin (TIF)
For fresh materials and those recovered from intestinal mucosal linings
Schaudinn’s fixative
For permanent staining
Schaudinn’s fixative
Enterobius vermicularis and Taenia spp.
CELLULOSE TAPE PREPARATION
• provides an estimate of worm burden
EGG COUNT TECHNIQUE
• for the recovery of hookworms, Ascaris, and Truchuris
EGG COUNT TECHNIQUE
- 1.5 mg feces (667) = epg
- 2.0 mg feces (500) = epg
Direct Smear Egg Count (Method by Beaver)
- uses 0.1 N NaOH
Dilution Egg Count
- Stoll’s Egg Counting Technique
Dilution Egg Count
- KTS for schistosomes
Thick Smear Egg Count
- Schistosomes
Dilution-Fitration Egg Count
• For accurate detection of the organisms as a supplement to other methods
CULTURE METHODS PURPOSES
• For obtaining a rich yield of organisms to be used as antigen in immunologic diagnosis
CULTURE METHODS PURPOSES
• For in-vitro screening of drugs
CULTURE METHODS PURPOSES
• For investigating the physiology of organisms
CULTURE METHODS PURPOSES
• As a source for inoculating susceptible experimental animals
CULTURE METHODS PURPOSES
• for Entamoeba histolytica and some other intestinal protozoa
Balamuth’s Monophasic Medlum
- Best medium
A. Novy, MacNeal and Nicole (NNN) Diphasic
