Hema (Stains)

Question 1

Stains ALP in neutrophil and to a small degree, in certain B cells.

Answer 1

Leukocyte Alkaline
Phosphatase

Question 1

Stains peroxidases present in granulocytes and monocytes

Answer 1

Peroxidase

Question 2

Stains lipids in monocytes and granulocytes

Answer 2

Sudan Black B

Question 3

Stains esterases present in monocytic cells, macrophages,
megakaryocytes and platelets

Answer 3

Non-specific esterase

Question 4

Stains esterases present in granulocytes

Answer 4

Chloroacetate
esterase

Question 5

Stain mucoproteins, glycoproteins, and high molecular weight
carbohydrates normally present in almost all blood types except
pronormoblast

Answer 5

Periodic Acid Schiff

Question 6

Diagnose Di Guglielmo’s syndrome and aid to classify some
acute leukemias

Answer 6

PAS

Question 7

Differentiated by LAP

Answer 7

Chronic myelogenous from
leukemoid reaction or PV

Question 7

Stains ACP present in the myelogenous cell, lymphocytes,
plasma cells, monocytes and platelets
L (+) tartaric acid – diagnose hairy cell leukemia

Answer 7

Acid phosphatase

Question 8

Differentiated by Peroxidase

Answer 8

Acute myelogenous and monocytic leukemia from
Acute lymphocytic leukemia

Question 9

Differentiated by SBB

Answer 9

Acute myelogenous and myelomonocytic leukemias from
Acute lymphocytic leukemia

Question 9

Differentiated by Chloroacetate esterase

Answer 9

Granulocytic cells from
Monocytic cells

Question 10

Differentiated by NSE

Answer 10

Monocytic leukemias from
Granulocytic leukemias

Question 10

Non-enzyme stains

Answer 10

Sudan Black B
PAS
Toluidine blue

Question 11

Marker for primary granules of
Auer rods

Answer 11

MPO

Question 12

Marker for phospholipids and lipids

Answer 12

Sudan Black B

Question 13

Stains DNA polymerase
immunoperoxidase

Answer 13

Terminal
deoxyribonucleotidyl
transferase (TdT)

Question 14

Marker for glycogen, glycoproteins,
mucoproteins and high molecular
weight carbohydrates

Answer 14

PAS

Question 15

Marker for monocyte,
megakaryocyte, and plasma cells

Answer 15

Alpha-naphthyl
acetate esterase

Question 15

Marker for mature and immature
neutrophils and mast cells

Answer 15

Naphthol AS-D
chloroacetate
esterase

Question 16

Identifying monocytes,
promonocytes, and monoblasts

Answer 16

Alpha- naphthyl
Butyrate esterase

Question 17

Present in all hematopoietic cells
and found on lysosomes

Answer 17

Acid phosphatase

Question 18

Marker for hairy cell leukemia

Answer 18

Tartrate resistant
acid phosphatase

Question 19

Neutrophils – only leukocyte that
contains this activity

Answer 19

Leukocyte alkaline
Phosphatase (LAP)

Question 20

Binds with acid mucopolysaccharides in blood cells

Answer 20

Toluidine Blue

Question 21

Present in 90% cases of ALL

Answer 21

TdT

Question 22

Peroxidase activity produces dark brown granules in cytoplasm of granulocytes and monocytes

Answer 22

MPO

Question 23

Dark purple black granules in neutrophil precursors Lymphoblasts are negative

Answer 23

SBB

Question 24

Activity results in bright fuchsia pink or magenta red Pattern of staining varies with each cell type

Answer 24

PAS

Question 25

Enzyme activity results in bright red granules in cytoplasm of neutrophils, neutrophil precursors and mast cells

Answer 25

Naphthol AS-D chloroacetate esterase

Question 26

Monocytic stain red-brown

Answer 26

Alpha-naphthyl acetate esterase

Question 27

Enzyme activity results in dark red precipitates in cytoplasm

Answer 27

Alpha- naphthyl Butyrate esterase

Question 28

Activity is indicated by purple to red granules

Answer 28

Acid phosphatase

Question 29

Activity is indicated by purple to dark red granules in cytoplasm

Answer 29

Tartrate resistant acid phosphatase

Question 30

100 cell count is done. Neutrophils are scored from with no activity to 4 with a large amount of activity

Answer 30

LAP

Question 31

Strongly metachromatic

Answer 31

Toluidine Blue

Question 32

nonspecific esterases

Answer 32

Alpha-naphthyl acetate esterase Alpha- naphthyl Butyrate esterase