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Year 1 NSF > Histology > Flashcards

Flashcards in Histology Deck (171)
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1

Define histology

The study of microscopic structures and tissues

2

Why is a high resolution needed?

To observe and discriminate between cell and tissue components

3

What are the 6 steps in tissue processing?

Fixation, embedding, sectioning, mounting, de-waxing, staining

4

What is the process of fixation?

Preserves and fixes specimens in place with crosslinks using formaldehyde

5

What is the process of embedding?

First dehydrate and remove water with alcohol, then clearing using organic solvent, then replacing with wax (58) or resin (60) for physical support

6

What is the process to sectioning?

Sectioned by a microtome to allow discrimination

7

What is mounting?

Mounting is the process of mounting tissues to a glass slide to be stained and viewed

8

What is de-waxing?

The removal of wax or resin to allow staining using organic solvent, then alcohol and then water

9

Why is staining needed?

to show and distinguish specific features.

10

What colour are the nucleus and cellular proteins with H and E stain?

DNA/RNA- purple/blue. Intra/extracellular proteins- red

11

What colour does trichrome stain the nuclei, intracellular and extracellular proteins?

Nuclei varies, intracellular proteins- red/pink, extracellular proteins- blue/green

12

What are both ordinary CT and cartilage made from?

Cells and extra cellular matrix

13

What is ordinary CT used for?

Binding muscle to bone and binding individual cells, and allows O2 to diffuse through extra cellular matrix and WBCs

14

What cells in ordinary CT secrete ECM?

Fibroblasts

15

What are mature and dormant fibroblasts called?

Fibrocytes

16

What features does a fibroblast cell have?

Prominent nucleus and granular cytoplasm

17

What is an adipocyte?

Fat cell

18

What are the two types of adipocytes?

White or brown

19

What are the functions of adipocytes?

Energy reserves- triglycerides, thermal insulation, shock absorber- spine, hooves, brown fat for rapid heat producer

20

What causes the pigment of brown fat?

The dense mitochondria to rapidly break down the fat and release heat

21

What type of fibres are in the ECM?

Collagen, Reticular, Elastic

22

What colour do collagen fibres stain with H&E and trichrome?

H&E- pink, Trichrome- Blue

23

Which fibre in ordinary CT is made from abundant proteins assembled into fibres?

Collagen- strong and give tensile strength

24

What doe reticular fibres form in the liver?

The internal skeleton

25

Which fibre in ordinary CT can recoil?

Elastic

26

Which fibre is needed to limit the stretching of elastic fibres?

Collagen fibres

27

What are proteoglycans in the ground substance made from?

GAGs or hydrated sugars attached to a protein core

28

What are the types of loose connective tissue?

Areolar, adipose and reticular

29

What are the types of dense connective tissue?

dense regular, dense irregular, elastic

30

What are the three types of cartilage?

hyaline, fibro and elastic