Histology (complete) Flashcards Preview

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Flashcards in Histology (complete) Deck (142)
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1

What are the steps in prepping a tissue for analyzing

1. obtain the tissue
2. quickly place in a fixative
3. sectioning the sample

2

what does a fixative do in preparing a tissue

1. coagulate cellular protein
2. harden gels
3. inactivate enzymes

3

What are the two main types of sectioning for a tissue sample

1. longitudinal cuts
2. transverse cuts

4

What is a longitudinal section

a cut that is made with the long axis of the tissue

5

What is a transverse section

a cut that is made perpendicular to the long axis of the tissue

6

What is the name of the most common histological stain

Hemotoxylin and Eosin (H&E)

7

What color is the nucleus of a cell going to be colored if a Hemotoxylin and Eosin stain is used

Blue

8

what color is the cytoplasm of a cell going to be if a Hemotoxylin and Eosin stain is used

Red or pink

9

What color will muscle and collagen be if a Hemotoxylin and eosin stain is used

Pink

10

What are the 4 types of tissues in the body

1. Epithelial
2. Connective
3. Muscular
4. Nervous

11

Where does epithelial tissue come from (what germ layer)

All Three germ layers

12

What is epithelial tissue

Closely adhering sheets of cells

13

Is epithelial tissue vascular

no, it is avascular

14

is epithelial tissue single layered or does it have multiple layers

it can be single layered or have multiple layers

15

what are the 4 main features of epithelial cells

1. Mostly uninucleate
2. Polyhedral
3. Polarized
4. undergo regular turnover

16

What does it mean that epithelial cells are polarized

that different sides of the cell are not the same. it has specific sides that do specific things

17

what are the specific sides of epithelial cells

Apical
Basal
Lateral

18

What is the apical end of epithilial cells (what does it border)

the apical end of the cell faces outward, away from the basement membrane

19

what is the basal end of epithelial cells (what does it border)

the basal end of the cell borders, or is up against the basement membrane

20

Where is the basement membrane usually found

between the epithelial tissue and underlying tissue

21

Do epithelial cells usually undergo regular turnover

yes

22

What is the basement membrane

it is an extracellular selective filter between epithelium and connective tissues

23

what kind of cells is the basement membrane made from

it isn't made from cells it is extracellular

24

What are the main components of the basement membrane

1. collagen (type 4 and 7)
2. proteglycans
3. laminin
4. entactin
5. fibronectin
6. underlying reticular fibers

25

What does the basement membrane control

1. cellular growth
2. cellular metabolism
3. cellular differentiation

26

What are the three layers of the basement membrane

1. lamina lucida
2. lamina densa (basal lamina)
3. lamina fibroreticularis

27

What are the two main types of collagen found in the basement membrane

type 4 and 7

28

What is another name for the lamina densa (middle layer of the basement membrane)

basal lamina

29

What is the order of the layers of the basement membrane from deepest to most superficial

lamina fibroreticularis
lamina densa (basal lamina)
lamina lucida

30

Where is epithelial tissue found

1. covers body surfaces (inside and out)
2. lines internal cavities
3. inside of various organs and glands
4. lines ducts