Immunology

Question 1

1) RID

2) Ouchterlony double diffusion

Answer 1

Mancini/endpoint method

Fahey&mckelvey/kinetic

Question 2

Both ag-ab diffuse independently thru a semi-solid medium
Serologic identity =
Non-identity =
Partial identity =

Answer 2

Ouchterlony DD
smooth curve
crossed line
spur-formation ; ab precip. ag w/ common determinant

Question 3

Precipitation by electrophoretic techniques

Answer 3

1. rocket immunoelectrophoresis
2. countercurrent immunoelectrophoresis
3. immunofixation electrophoresis

Question 4

Rocket

Answer 4

RID + EP

Height/apex = Ag conc

Question 5

Countercurrent EP
Source of Ag
Sign of abnormality

Answer 5

Px serum wc is EPd in separate protein fractions

Change in shapre, loc, and intensity of precipitin arc

Question 6

Phases of agglu

Answer 6

Sensi

Lattice form

Question 7

enhancement oflattice f

Answer 7

LISS, PEG, enz, pH, adjusting temp

Question 8

Types of agglu

Answer 8

Direct
Passive
Reverse Passive
Coagglu
Agglu Inhibition
Antiglobulin-mediated agglu

Question 9

Direct agglu

Ex.

Answer 9

Ex. Hemagglu = Ag is found on RBCs

Question 10

Passive agglu

Ex.

Answer 10

Ex. ASO latex agglutination test

Question 11

Ag on surface of particle

Answer 11

Direct agglu

Question 12

Ag is attached to the carrier particle

Answer 12

Passive agglu

Question 13

Agglu occurs if px Ab is present

Answer 13

Passive agglu

Question 14

Ab is attached to the c.part

Answer 14

Reverse passive agglu

Question 15

Agglu occurs if px ag is present

Answer 15

Reverse passive agglu

Question 16

Rev-Pass agglu

ex.

Answer 16

CRP LAT

Question 17

Uses bacte as inert particles to wc ab are attached

Answer 17

coagglu

Question 18

Positive result is lack of agglu

Answer 18

agglu inhib

Question 19

agglu inhi ex.

Answer 19

B HCG
Hemagglu inhi (influenza, rubella, measles)

Question 20

Antiglobulin-mediated agglu ex.

Answer 20

Coomb’s
DAT
IAT

Question 21

DAT

IAT

Answer 21

Px RBCs + AHG = agglu (+)

sensi thru incubation -> wash 2-3x -> AHG

Question 22

analyte measured competes w/ a radiolabeled analyte for a limited no. of binding sites

Answer 22

compet RIA

Question 23

amt of label is inv propo to the amt of px ag

Answer 23

compet RIA

Question 24

amt of bound labelled Ab is directly propo to the amt of px analyte

Answer 24

non compet IRMA

Question 25

labeled immunoassays

Answer 25

RIA EIA Fluorescent immunoassay

Question 26

EIA

Answer 26

horse radish perox g6pd glucose oxidase b-galactosidase

Question 27

based on princip of RIA

Answer 27

compet ELISA

Question 28

enzyme act is indirectly prop to the conc of analyte

Answer 28

compet ELISA

Question 29

indirect ELISA

Answer 29

non compet ELISA

Question 30

enzyme-labeled rgt does not participate to the initial ag-ab rxn

Answer 30

non compet ELISA

Question 31

amt of enzyme label is direct propo to the con of analy

Answer 31

non compet elisa

Question 32

enzyme act is directly propo to the ag conc

Answer 32

sandwich/capture EIA

Question 33

direct immunof assay

Answer 33

unknown ag + FITC/Tritc labeled ab = + fluor

Question 34

indirect IF

Answer 34

known ag + unknown ab + fitc/titc labeled AHG = + fluor