Introduction To Histology

Question 1

What is the most commonly used dye combination

Answer 1

Haematoxylin and Eosin (H&E)

Question 2

What colour does haematoxylin and eosin stain cytoplasm and nuclei

Answer 2

Nuclei stain blue and cytoplasm stains pink

Question 3

What also stains pink when using a H&E stain

Answer 3

Most extra-cellular fibres such as collagen and elastic stain pink with H&E stain

Question 4

What is PAS used to stain

Answer 4

Sugars

Question 5

What is van Gieson used to stain

Answer 5

Elastic

Question 6

What is Alcian blue used to stain

Answer 6

Mucins

Question 7

What is trichromatic stain used for

Answer 7

It is used to stain 3 types of cells to visualise the difference between them

Question 8

What is an example of a small cell

Answer 8

A lymphocyte which is about 10 micrometers in diameter

Question 9

What is an example of a large cell

Answer 9

A motor neuron which is about 100 micrometers wide with axons up to 1 meter in length

Question 10

What shape are these cells

Answer 10

Rounded

Question 11

What shape are these cells

Answer 11

Polygonal

Question 12

What shape are these cells

Answer 12

Fusiform

Question 13

What shape are these cells

Answer 13

Columnar

Question 14

What shape are these cells

Answer 14

Cuboidal

Question 15

What shape are these cells

Answer 15

Squamous

Question 16

In general what is the difference between metabolically active and inactive cells

Answer 16

Metabolically active cells are larger than metabolically inactive cells

Metabolically active cells often have nucleoli

Question 17

What is the life span of tissues lining the gut

Answer 17

Days

Question 18

What is the life span of tissues such as blood, skin and connective tissues

Answer 18

Months

Question 19

What is the lifespan of bones and tendons

Answer 19

Years

Question 20

What is the life span of skeletal muscle

Answer 20

Nearly your whole life

Question 21

What is the life span of nerves, brain, cardiac muscle and germ cells

Answer 21

They last your whole life

Question 22

What are the arrows pointing at

Answer 22

Nucleolus

Question 23

What is this showing

Answer 23

Mitochondria

Question 24

What is this showing

Answer 24

Rough endoplasmic reticulum

Question 25

What is this showing

Answer 25

Smooth endoplasmic reticulum

Question 26

What is this showing

Answer 26

Golgi apparatus

Question 27

What is this showing

Answer 27

Lysosomes

Question 28

How big are microfilaments and what is an example

Answer 28

Microfilaments are about 5 micrometers Actin is an example of a Microfilament

Question 29

How big are microtubules and what is an example

Answer 29

Microtubules are 25 micrometers Tubulin is an example

Question 30

What calls don’t have any microtubules in

Answer 30

Erythrocytes

Question 31

How big are intermediate filaments

Answer 31

10 diameters

Question 32

Where are the intermediate filaments cytokeratins found

Answer 32

Epithelial cells

Question 33

Where are the intermediate filament desmin found

Answer 33

Myocytes

Question 34

Where are the intermediate filaments glial fibrillary acidic proteins found

Answer 34

Astrocytic glial cells

Question 35

Where are the intermediate filaments neurofilament proteins found

Answer 35

Neurons

Question 36

Where are the intermediate filaments nuclear laminin found

Answer 36

Nuclei of all cells

Question 37

Where are the vimentin intermediate filaments found

Answer 37

Mesodermal cells

Question 38

What are the different types of tissues

Answer 38

Epithelial tissue Muscle tissue Supporting tissue Nerve tissue Germ cell tissue