L1 HISTOMTLE Flashcards
Commercial stock formaldehyde solutions contain:
A. 4% formaldehyde
B. 10% formaldehyde
C. 37% to 40% formaldehyde
D. 98% to 100% formaldehyde
C. 37% to 40% formaldehyde
10% formalin contains approximately what percentage of formaldehyde?
A. 4
B. 10
C. 37
D. 100
A. 4
A microwave oven can be used for fixation because it:
A. Causes cross-linking of proteins
B. Induces physical fixation
C. Increases tissue basophilia
D. Inactivates enzymes with beta-radiation
B. Induces physical fixation
This organ is usually suspended whole in 10% buffered formalin for 2 to 3 weeks to ensure fixation and some hardening prior to sectioning.
A. Lungs
B. Liver
C. Brain
D. Amputated foot
C. Brain
Fixatives that give the best results with metachromatic staining and are the routine fixatives of choice for preservation of detail for photography:
A. Formalin fixatives
B. Mercurial fixatives
C. Lead fixatives
D. Chromate fixatives
B. Mercurial fixatives
Airholes found on tissue
A. Incomplete fixation
B. Incomplete dehydration
C. Incomplete clearing
D. Incomplete impregnation
D. Incomplete impregnation
Tissue is opaque, section cutting is difficult due to the presence of alcohol.
A.Incomplete fixation
B.Incomplete dehydration
C.Insufficient clearing
D. Incomplete impregnation
C.Insufficient clearing
Tissue is soft when block is trimmed:
A.Incomplete fixation
B.Incomplete dehydration
C.Incomplete clearing
D.Incomplete impregnation
A.Incomplete fixation
The first and most critical step in histotechnology?
A. Fixation
B. Clearing
C. Infiltration
D. Embedding
A. Fixation
These knives are used to cut block for ELECTRON MICROSCOPY (EM):
A. Disposal blades
B. Steel knives
C. Diamond or glass knives
D. Magnetic blades
C. Diamond or glass knives
Microtome knives for PARAFFIN SECTIONING are usually made of:
A. Carbon steel
B. Diamond
C. Glass
D. Iron
A. Carbon steel
It is considered to be as the best vital dye.
A. Janus green
B. Neutral red
C. Nile blue
D. Toluidine blue
B. Neutral red
It is used for demonstrating mitochondria during vital staining:
A. Janus green
B. Neutral red
C. Nile blue
D. Toluidine blue
A. Janus green
Oldest stain:
A. Orcein
B. Iodine
C. Eosin
D. Hematoxylin
B. Iodine
What is the most sensitive lipid stain?
A. Oil red O
B. Sudan III
C. Sudan IV
D. Sudan Black B
D. Sudan Black B
The phosphotungstic acid hematoxylin (PTAH) is useful for demonstrating:
A. Edema fluid
B. Muscle striations
C. Ground substance
D. Reticulin network
B. Muscle striations
The connective tissue cells actively involved in wound healing are:
A. Plasma cells
B. Mast cells
C. Macrophages
D. Fibroblasts
D. Fibroblasts
Due to chronic trauma induced by ill-fitting dentures:
A. Hypertrophy
B. Hyperplasia
C. Dysplasia
D. Metaplasia
B. Hyperplasia
It is a special form of cell death produced by the TUBERCLE BACILLUS.
A. Coagulation necrosis
B. Liquefaction necrosis
C. Fat necrosis
D. Caseous necrosis
D. Caseous necrosis
Cell death due to ischemia is known as Infarction and is manifested by a characteristic histologic appearance which is:
A. Coagulation necrosis
B. Liquefaction necrosis
C. Fat necrosis
D. Caseous necrosis
A. Coagulation necrosis
Which of the following organs should not be dissected before they are fixed?
A. Eyes
B. Lungs
C. Heart
D. Liver
A. Eyes
Formalin fixes tissue by:
A. Causing a loss of color
B. Forming crosslinks
C. Forming formic acid
D. Facilitating numerous staining techniques
B. Forming crosslinks
The primary aim of fixation:
A. Preserve the morphologic and chemical integrity of the cell
B. Act as mordant or accentuators
C. Harden and protect the tissue from trauma of further handling
D. Inhibit bacterial decomposition
A. Preserve the morphologic and chemical integrity of the cell
The secondary goal of fixation:
A. Preserve the morphologic and chemical integrity of the cell
B. Act as mordant or accentuators
C. Harden and protect the tissue from trauma of further handling
D. Inhibit bacterial decomposition
C. Harden and protect the tissue from trauma of further handling