L14 Vesicular Trafficking I Flashcards Preview

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Flashcards in L14 Vesicular Trafficking I Deck (36)
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1

What types of SNAREs are there

v-SNAREs and t-SNAREs

2

What are transport vesicles coated in?

Clathrin, COPI & COPII

3

What is a paradigm for transport vesicle formation

COPII coated vesicle

4

What associates with the rough ER

ribosomes

5

How are newly made proteins translocated into organelles

co-translationally or post-translationally

6

What does translocation into the ER require

signal

7

How are membrane proteins inserted into the ER

signal peptidase

8

What ensures proper protein folding in the ER

chaperones

9

What do defects in protein folding give rise to

disease

10

What do misfolded proteins stimulate?

unfolded protein response

11

What are the essential components for all transport vesicle formation

GTPase, adaptor proteins, and coat

12

What act as molecular switches

GTPases

13

What converts GDP to GTP

guanine nucleotide exchange factors

14

What converts GTP to GDP

GTPase activating proteins

15

Where is GDP form usually found

cytosol

16

Where is GTP form usually found

membrane

17

What is the main member of GTPases

Ras

18

What is the GTPase component of COPII

Sar1

19

What is the adaptor component of COPII

Sec23/24

20

What is the coat component of COPII

Sec13/31

21

Adaptor protein function

recognise and select cargo ensuring specificity

22

What do adaptor proteins recognise

motifs in cytoplasmic domains

23

AP2

major clathrin adaptor

24

What is AP2 involved in

endocytosis

25

What do coat proteins provide

structural scaffold

26

What experiments allow us to understand what makes a COPII vesicle

reconstitution experiments

27

Ribophorin

resident ER protein

28

P58

COPII cargo protein

29

Where are GTPases recruited from

cytosol

30

GTP mutant

cannot hydrolyse GTP