Flashcards in L14 Vesicular Trafficking I Deck (36)
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1
What types of SNAREs are there
v-SNAREs and t-SNAREs
2
What are transport vesicles coated in?
Clathrin, COPI & COPII
3
What is a paradigm for transport vesicle formation
COPII coated vesicle
4
What associates with the rough ER
ribosomes
5
How are newly made proteins translocated into organelles
co-translationally or post-translationally
6
What does translocation into the ER require
signal
7
How are membrane proteins inserted into the ER
signal peptidase
8
What ensures proper protein folding in the ER
chaperones
9
What do defects in protein folding give rise to
disease
10
What do misfolded proteins stimulate?
unfolded protein response
11
What are the essential components for all transport vesicle formation
GTPase, adaptor proteins, and coat
12
What act as molecular switches
GTPases
13
What converts GDP to GTP
guanine nucleotide exchange factors
14
What converts GTP to GDP
GTPase activating proteins
15
Where is GDP form usually found
cytosol
16
Where is GTP form usually found
membrane
17
What is the main member of GTPases
Ras
18
What is the GTPase component of COPII
Sar1
19
What is the adaptor component of COPII
Sec23/24
20
What is the coat component of COPII
Sec13/31
21
Adaptor protein function
recognise and select cargo ensuring specificity
22
What do adaptor proteins recognise
motifs in cytoplasmic domains
23
AP2
major clathrin adaptor
24
What is AP2 involved in
endocytosis
25
What do coat proteins provide
structural scaffold
26
What experiments allow us to understand what makes a COPII vesicle
reconstitution experiments
27
Ribophorin
resident ER protein
28
P58
COPII cargo protein
29
Where are GTPases recruited from
cytosol
30
GTP mutant
cannot hydrolyse GTP
31
GDP mutant
sequester GEFs
32
Mutant GTPases effect
dominant negative
33
Expression of Sar1GDP inhibits
COPII formation
34
COPII cargo
newly synthesised proteins
35
COPI cargo
retrieved & newly synthesised protein
36
