Yeast Screens (L13?) Flashcards Preview

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Flashcards in Yeast Screens (L13?) Deck (30)
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1

Enzyme modifications

glycosylation and proteolytic cleavage

2

What are the major membrane trafficking pathways

secretory/exocytic pathway, endocytic pathway

3

What is NOT part of the secretory pathway

mitochondria

4

What does the secretory pathway involve

ER to golgi to PM/endosome/lysosome

5

What does the endocytic pathway involve

cell surface to endosome to golgi/ER/lysosom

6

Protein modification as they transit ER and golgi

glycosylated and proteolytically cleaved

7

Advantage of yeast as model organism for membrane trafficking studies

genome sequenced, cheap and easy to grow, large quantities

8

Disadvantages of yeast as model organism

limited cell-cell contact, small, cell wall

9

Aim of Novick and Schekman experiment in 1980

investigate secretory pathway in yeast

10

Secretory mutants

fail to export active invertase and acid phosphatase

11

Difference in mutant appearance

accumulation of vesicles, aberrant membranous structures

12

How many genes are involved in transport of proteins to membrane

23

13

Where do secreted proteins from class A sec genes accumulate

cytosol

14

Where do secreted proteins from class B sec genes accumulate

rough ER

15

Where do secreted proteins from class C sec genes accumulate

ER to golgi transport vesicles

16

Where do secreted proteins from class D sec genes accumulate

golgi

17

What genes did Novick and Schekman miss?

genes involved in transport to endosome or vacuole, redundantly functioning genes

18

Where is the decision made to traffic to surface or lysosome

TGN

19

What is endocytosis

PM invaginates into the cell - vesicle production that fuses with endosome

20

Endocytosis functions

down-regulation of signals, remodelling cell surface composition

21

What is the first stage of endocytosis

PM to endocytic vesicle

22

What is the last stage of endocytosis

late endosome to golgi or vacuole

23

What is important in yeast endocytosis

actin cytoskeleton

24

What is the major function of lysosomes

degradation of extracellular material

25

What do lysosomes contain

degradative/proteolytic enzymes

26

What is carboxypeptidase

vacuolar enzyme

27

where is carboxypeptidase transported from

ER to golgi

28

How is carboxypeptidase sorted

by receptors

29

What does the transport step of sorting require

cytoplasmic factors

30

What cytoplasmic factors does the transport step of sorting require

clathrin and two adaptors called Gga1 Gga2