L4: Pneumonia - Pt 3 Flashcards
(46 cards)
Labs in Pneumonia
Labs in Pneumonia
- Detection of The Microorganism
Sputum Sample in Pneumonia
Detection of the microorganism in Pneumonia
- After clinical & radiological diagnosis of pneumonia the following samples must be taken
Sputum Sample in Pneumonia
- When??
If there is productive cough
Sputum Sample in Pneumonia
- For??
Culture and Gram stain
Blood Sample in Pneumonia
Sputum Sample in Pneumonia
- Drawbacks
Contamination is inevitable with the oropharyngeal commensals.
Sputum Sample in Pneumonia
- Quality Criteria
- Not a salivary specimen
- The number of neutrophil > 25 cell
- The buccal epithelial cells are < 10 cell/low power field (x100).
Blood Sample in Pneumonia
- For??
- Blood culture
(actual infective organism(s) with no contamination).
Blood Sample in Pneumonia
- Results
+ve in 10 - 30% of cases only.
Pleural Aspirate in Pneumonia
Pleural Aspirate in Pneumonia
- when??
If there pleural effusion
Pleural Aspirate in Pneumonia
- For??
Culture & Gram stain (no contamination)
Pleural Aspirate in Pneumonia
- Results
If +ve it indicate presence of empyema.
Detection of Microorganism in Pneumonia
- Absence of organism by culture or Gram stain in sputum samples may suggest infection by ……..
Detection of Microorganism in Pneumonia
- Special stains
Special Stains in Pneumonia
- ZN Stain
For TB bacilli
Special Stains in Pneumonia
- Fungal Wet Mount Smears
For fungal infections (need fluorescent agent or phase-contrast microscopy)
Special Stains in Pneumonia
- Wright Stain
To detect aspergillus infection
Special Stains in Pneumonia
- giesa & Quik Stain
For pneumocystis carinii.
Detection of Microorganism in Pneumonia
- Immunostains
Immunostains in Pneumonia
- detection of Pneumococcal Antigen
- By Latex agglutination test
- Counter Immune-Electrophoresis (CIE)
- ELISA.
Immunostains in Pneumonia
- Direct Immunoflouresent Antibody Test
- For Legionella
- Pneumocystis carinii
- Chlamydia.