LAB 9 - HCG

Question 1

compound Produced by the trophoblast cells of the placenta during pregnancy

Answer 1

HUMAN CHORIONIC GONADOTROPIN

Question 2

human chorionic gonadotropin is a dimeric molecule which consists of

Answer 2

alpha sub unit and beta sub unit

Question 3

is the HUMAN CHORIONIC GONADOTROPIN a glycoprotein?

Answer 3

yes po

Question 4

Same Alpha Sub-unit is analogous to

Answer 4

LH, FSH, TSH

Question 5

Same beta Sub-unit is analogous to

Answer 5

LH

Question 6

Normal concentrations of LH, FSH, and TSH would not actually have significant effect on the qualitative test for HCG
or pregnancy testing.

t or f

Answer 6

true - it ha sthreshold

Question 7

HCG

Serves to maintain ___ production by the corpus luteum in the early pregnancy

Answer 7

progesterone

Question 8

___ is the predominant form during pregnancy

Answer 8

Intact HCG

Question 9

HCG is can be detected after how many days of ovulation

Answer 9

2-3 days

Question 10

For serum assay, an antibody is directed separately to _____sub-unit and intact HCG

Answer 10

beta

Question 11

the one being detected in serum assay is the

Answer 11

beta sub unit and the intact hcg

Question 12

For the urine assay, what is specifically measured is the ___

Answer 12

alpha and beta subunit of HCG.

Question 13

Qualitative test for urine samples has detection limits of about __

Answer 13

50 mIU/mL

Question 14

the test kits we use can have a sensitivity as low as ___ mIU/mL

Answer 14

25 - meaning it’s easier to trigger or detect a positive reaction even at a smaller concentration

Question 15

Serum HCG increases above reference interval (4 – 6 mIU/mL) by __

Answer 15

implantation

Question 16

Indicative of pregnancy or trophoblastic disease

Answer 16

> 5 mIU/mL

Question 17

Serum level during the first trimester of pregnancy

Answer 17

> 100,000 mIU/mL

Question 18

HCG levels increase to more than 100 mIU/mL. It can go as high as that. Hence, there is a
significant color change and the appearance of a line on our test kit.

what could be the indication

Answer 18

pregnancy at first trimester

Question 19

antibody-antigen-antibody setup to detect the presence of a
particular antigen.

Answer 19

sandwich assay - immunometric method

Question 20

The presence of an antigen between the two antibodies will
trigger or create the colorimetric reaction.

Answer 20

immunochromatographic
method using a sandwich technique

Question 21

SD HCG One Step Pregnancy Test

what is its sensitivity

Answer 21

25 mIU/ml

Question 22

As early as the first day of a missed period, it can readily provide a positive result for individuals who are pregnant. On
the first day of a missed period, it is estimated that the HCG level is around 100 mIU/mL.

Answer 22

SD HCG One Step Pregnancy Test

Question 23

amount of HCG in 10-12 weeks into pregnancy

Answer 23

30,000-100,000 mIU/L

Question 24

in the Test Kit

what is the component of the gold conjugate

Answer 24

Mouse Anti-beta hCG monoclonal antibody-gold colloid

Question 25

in the Test Kit what is the component of the test line

Answer 25

goat anti-hcg

Question 26

in the Test Kit what is the component of the control line

Answer 26

goat anti mouse IgG

Question 27

specimen for HCG

Answer 27

first morning urine specimen

Question 28

Urine samples containing visible precipitate should be __

Answer 28

centrifuges or allowed to settle

Question 29

if the sample is stored in refrigerator, it can be kept for __ hrs

Answer 29

72 hrs

Question 30

if the sample is stored in freezer, it can be kept for ___

Answer 30

14 days

Question 31

in using the test kit, we squeeze how many drops of urine in the well

Answer 31

3-4 drops

Question 32

we interpret the results at how many mns

Answer 32

3 mns

Question 33

Negative Result -->

Answer 33

the presence of only one purple band (“C”) within the test window

Question 34

Positive Result ->

Answer 34

® the presence of two color bands (“I”) and (“C”) within the result window

Question 35

Invalid Results -->

Answer 35

® if the purple color band (“C”) is not visible within the result window

Question 36

threshold for LH as an interference

Answer 36

200 mIU/ml

Question 37

threshold for FSH as an interference

Answer 37

1000 mIU/ml

Question 38

threshold for TSH as an interference

Answer 38

1000 uIU/ml

Question 39

SUBSTANCES (20 mg/dL) list the interferences in this threshold

Answer 39

* Acetaminophen * Acetylsalicylic Acid * Ampicillin * Ascorbic Acid * Atropine * Caffeine * Gentisic Acid * Phenylpropanolamine * Salicylic Acid * tetreacyline

Question 40

glucose has an interference of

Answer 40

2000 mg/dl

Question 41

hemoglobin has an interference of

Answer 41

25 mg/dl

Question 42

albumin has an interference of

Answer 42

2000 mg/dl