LEC 7 - PHOSPHOROUS

Question 1

It is an important constituent in nucleic acid, phospholipid
and phosphoproteins,

Answer 1

PHOSPHORUS

Question 2

phosphorus’s distribution

Answer 2

80% in the bone
20% in soft tissues
1% in plasma

Question 3

phosphorous concentration is inversely related to

Answer 3

calcium

Question 4

if the calcium is maximally absorbed in duodenum, what about phosphorus?

Answer 4

jejunum

Question 5

role of phsphate in glucose and insulin processing

Answer 5

essential for the insulin-mediated entry of glucose into the cells by a process involving PHOSPHORYLATION of the glucose and the co entry of K+

Question 6

in PHOSPHORUS we are determining __

Answer 6

phosphate

Question 7

inorganic phosphorous is called

Answer 7

phosphates

Question 8

Phosphorous

It forms high energy compounds such as ______and is involved in intermediary metabolism and
various enzyme systems

Answer 8

ATP
nucleic acid (dna and rna)
enzyme cofactors (NADPH)
lipids (phospholipids)

Question 9

most phosphate in serum is in what form

Answer 9

inorganic form

Question 10

It is essential for muscle contractility, neurologic function,
and electrolyte transport and oxygen-carrying by
hemoglobin.

Answer 10

PHOSPHORUS

Question 11

is the primary factor that increase phosphate

Answer 11

Vitamin D

Question 12

relationship of PTH to phosphate

Answer 12

inversely - PTH want calcium

Question 13

hyperphosphatemia usual cause

Answer 13

Decrease renal excretion in acute and chronic renal failure

Question 14

Increase intake with excessive oral, rectal, intravenous
administration.

hyper or hypo?

Answer 14

hyperphosphatemia

Question 15

Increase extracellular load due to transcellular shift in acidosis in phosphate

Answer 15

hyperphosphatemia

Question 16

Secondary to over medication with Vitamin D and
production of Vitamin by granulomatous tissue.

effect to phosphate

Answer 16

hyperphosphatemia

Question 17

phosphate is usually seen in children ___

Answer 17

drinking cow’s milk

Question 18

phosphate is usually seen in adult ___

Answer 18

taking laxatives

Question 19

patients with lymphoblastic leukemia

hyperphosphatemia or hypo?

Answer 19

hyperphosphatemia

Question 20

Hypoparathyroidism

hyperphosphatemia or hypo?

Answer 20

hyper

Question 21

t is a condition characterized by a serum phosphorus
concentration below the lower limit of the reference interval

Answer 21

HYPOPHOSPHATEMIA

Question 22

HYPOPHOSPHATEMIA can be seen in

Answer 22

o Alcohol abuse
o Intestinal loss due to vomiting, diarrhea, and use of phosphate binding antacids
o Induced by a shift of phosphorus from extracellular fluid into cells.

Question 23

decreased intestinal absorption, as with vitamin D
deficiency or antacid use and steatorrhea

hyperphosphatemia or hypo?

Answer 23

hypo

Question 24

Most of the current methods for phosphorus determination
involve the formation of an ____

Answer 24

ammonium
phosphomolybdate complex

Question 25

an ammonium phosphomolybdate complex. o This colorless complex can be measured by ultraviolet absorption at _____ nm

Answer 25

340

Question 26

* Most of the current methods for phosphorus determination involve the formation of an ammonium phosphomolybdate complex. o This colorless complex can be measured by ultraviolet absorption at 340 nm or can be reduced to form molybdenum blue, a stable blue chromophore, which is read between _____ nm.

Answer 26

600 and 700

Question 27

Reaction of phosphate with ammonium molybdate (_____)

Answer 27

FISKE SUBBAROW

Question 28

REFERENCE INTERVAL for phosphorous

Answer 28

Inserts * Adult: 2.8-4.5 mg/dL (0.89-1.44mmol/L) * Children: 4.0-7.0mg/dL (1.29-2.26mmol/L)

Question 29

the type of phosphate that is the principal anion within cells

Answer 29

organic phosphate

Question 30

the type of phosphate that is pary pf the blood buffer system

Answer 30

inorganic phosphate - kaya ang highest type of phosphate na makikita sa serum is si inorganic kasi pang buffer siya

Question 31

free or unbound form of phosphate percentage

Answer 31

55%

Question 32

complexed with ions percentage of phosphate

Answer 32

35%

Question 33

protein bound percentage of phospahte

Answer 33

10%

Question 34

role of PTH in phosphate

Answer 34

secretes phosphate - decrease

Question 35

role of growth hormone in phosphate

Answer 35

reabsorption - increase

Question 36

do we need to have the patient undergo fasting for phosphate determination?

Answer 36

yes, a high CHO can result in decreased levels

Question 37

what is the time (circadian rhythm) when phosphate has high production

Answer 37

late morning low levels in the evening

Question 38

effect of physical activty to phosphate

Answer 38

increase

Question 39

the most commonly used method to measure serum inorganic phosphate

Answer 39

fiske-subbarow method

Question 40

most common reagent in fiske subbarow

Answer 40

PICTOL (amino naphthol sulfonic acid )

Question 41

end product of fiske subbarow

Answer 41

ammonium molybdate complex

Question 42

the reduced form of the end product yield what color

Answer 42

blue

Question 43

major cause of hypophosphatemia

Answer 43

transcellular shift

Question 44

most common cause of hypophosphatemia

Answer 44

alcohol abuse