Lab - Phlebotomy & Protein-free Filtrates Flashcards Preview

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Flashcards in Lab - Phlebotomy & Protein-free Filtrates Deck (19):
1

[Phlebotomy]
identify
the act of obtaining a blood sample from a vein using a needle attached to a syringe or an evacuated tube

phlebotomy

2

[Phlebotomy]
identify the best site for venipuncture, and its specific location in the body

median cubital vein in antecubital fossa

3

[Phlebotomy]
Which is true?
A.Venous blood is easy to get & it has most analytes in soluble form.
B.Evacuated tube method is the same as syringe method.
C.BOTA
D.NOTA

A

4

[Phlebotomy]
What is the foremost essential factor in providing reliable lab results?

proper patient identification

5

[Phlebotomy]
Which is true?
A. Once blood collection is done,you must allow patient to flex his/her arm.
B. A tourniquet must not be applied for more than a minute.
C. BOTA
D. NOTA

B

6

[Phlebotomy]
How is protein removed from the blood and other biological fluids before carrying out the tests?

precipitated by either acids or salts of heavy metals

7

[Phlebotomy]
Once protein is precipitated, what is the clear liquid called?

protein-free filtrate

8

[Protein-Free Filtrates]
Which is false?
A. The precipitating agent is the same regardless of the analysis procedure.
B. The precipitating agent should not interfere with the substance being determined.
C. BOTA
D. NOTA

A

9

[Protein-Free Filtrates]
The precipitating agent should provide proper __ for the filtrate.
A. pressure
B. weight
C. pH
D. AOTA

C

10

[Protein-Free Filtrates]
Enumerate 3 sources of error in PFF preparation.

use of unclean glassware
incorrect volumes
foaming

11

[Protein-Free Filtrates]
foaming indicates
A.inadequate sample weight
B.incomplete protein precipitation
C.too vigorous shaking
D.NOTA

B

12

[Protein-Free Filtrates]
Enumerate 3 causes of foaming

improper reagent concentration
presence of an excess of anticoagulant
use of improper anticoagulant

13

[Protein-Free Filtrates]
Biological fluids are precipitated with either acids or salts of heavy metals. The resulting suspension is cleared of solids by either of which 2 methods?

centrifugation
filtration

14

[Protein-Free Filtrates]
Enumerate 3 methods of protein-free filtrate preparation.

Folin Wu's Method
Somogyi Method
Trichloroacetic Method

15

[Protein-Free Filtrates]
Another name for Folin Wu's Method is ___

Tungstic Acid Filtrate

16

[Protein-Free Filtrates]
To which method does this apply: "H2SO4 + 10% sodium tungstate is added to the sample"
A.Folin Wu's Method
B.Somogyi Method
C.Trichloroacetic Method

A

17

[Protein-Free Filtrates]
To which method does this apply: "The resulting protein free filtrates is extremely acid, therefore its use is limited to determinations requiring very high pH"
A.Folin Wu's Method
B.Somogyi Method
C.Trichloroacetic Method

C

18

[Protein-Free Filtrates]
To which method does this apply: "Protein is precipitated by use of zinc sulfate and barium hydroxide."
A.Folin Wu's Method
B.Somogyi Method
C.Trichloroacetic Method

B

19

[Protein-Free Filtrates]
False about Somogyi Method
A. Advantage: non-glucose reducing agents are not present
B. There should be a balance between conc of zinc sulfate & conc of hydroxide solution
C. This method can be used in determining uric acid, creatinine and non-protein nitrogen.
D.NOTA

C

cannot dapat; these are left with the coagulum