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Flashcards in Lab test 1 Deck (54):
0

Eye piece

Ocular lense

1

Magnify the specimen-rotate

Objective lenses

2

Attaches objective lenses to microscope

Revolving nosepiece

3

Where the slide is placed

Mechanical stage

4

Adjust light

Iris diaphragm lever

5

What you hold microscope with

By arm

6

Larger knob

Coarse adjustment

7

Small adjustment

Fine adjustment

8

Moves stage back and forth

Mechanical stage control

9

The enlargement of te image by use of the various objective and the ocular

Magnification

10

Ability to distinguish between two adjacent points

Resolution

11

The diameter of the smallest objective visible

Resolving power

12

Sphere or round

Coccus

13

Rods

Bacillus

14

Helixes or spiral

Spirillum

15

Total magnification=

Objective power x 10

16

Growth of cells of one specific microorganism

Pure culture

17

A medium may be a

Liquid or soli

18

Solid medium will have

Agar

19

Must be heated to boiling to dissolve and will solidify at 40C

Agar

20

Infection origination in a hospital

Nosocomial Infection

21

_____ a slide of bacteria improves the contrast between the cells and the background

Staining

22

Disadvantage of staining?

Drying and fixation of the cells onto a slide introduces some shrinkage and distortion to cell

23

Will kill the cells and also ensure that they will stay attached tithe glass surface during staining

Fixation

24

Distinguish between different organisms on the basis of staining reactions

Differential stains

25

Most useful differential stain in microbiology

Gram stain

26

Differentiates cells on te basis of cell wall composition

Gram stain

27

No lipid in their cell wall
Decolonize with ethanol or acetone
Retain crystal violet
Purple

Gram positive

28

Decolonize and accept the counterstain
Appear pink

Gram negative

29

What type of bacteria do you do acid stain for?

Mycobacterium
Nocardia
Staphylococcus

30

Acid dye that stains background

Negative stain

31

Used a modified negative stain with both the background and cells being stained

Capsule stain

32

_____ remains unstained, giving the cells a halo appearance

Capsule stain

33

Uses heat to penetrate the spore with the dye

Endospore stain

34

All over flagella

Peritrichous

35

Multiple flagella on one end

Polar lophotrichous

36

1 flagella on one end

Polar monotrichous

37

Flagella on both ends

Polar Amphitrichous

38

Uses a needle

Motility test

39

Indicated by growth away from the line of inoculation

Motility test

40

Steps of motility test

Heat needle
Obtain organism
Stab straight down 1 time

41

Enzymes that function outside the cell

Exoenzymes

42

Enzymes that function inside the cell

Endoenzyme

43

Collects gas produced during fermentation

Durham tube

44

Used in the fermentation of lactose

Durham tube

45

Anaerobic process

Fermentation of lactose

46

Test tube that has a second inverted tube inside used to capture fermented gas

Durham tube

47

One or more components that inhibit most microorganisms and allows a select group to grow

Selective media

48

Contain indicators that allow the distinguishing of different organisms on the basis of their appearance on the media or the color of the media around the growth

Differential media

49

Mannitol salt agar

Selective
Differential

50

MacConkey's Agar

Selective
Differential

51

Blood agar

Differential

52

Lowenstein-Jensen

Selective

53

Sabouraud's dextrose agar

Selective