Lab test 1

Question 0

Magnify the specimen-rotate

Answer 0

Objective lenses

Question 1

Eye piece

Answer 1

Ocular lense

Question 2

Attaches objective lenses to microscope

Answer 2

Revolving nosepiece

Question 3

Where the slide is placed

Answer 3

Mechanical stage

Question 4

Adjust light

Answer 4

Iris diaphragm lever

Question 5

What you hold microscope with

Answer 5

By arm

Question 6

Larger knob

Answer 6

Coarse adjustment

Question 7

Small adjustment

Answer 7

Fine adjustment

Question 8

Moves stage back and forth

Answer 8

Mechanical stage control

Question 9

The enlargement of te image by use of the various objective and the ocular

Answer 9

Magnification

Question 10

Ability to distinguish between two adjacent points

Answer 10

Resolution

Question 11

The diameter of the smallest objective visible

Answer 11

Resolving power

Question 12

Sphere or round

Answer 12

Coccus

Question 13

Rods

Answer 13

Bacillus

Question 14

Helixes or spiral

Answer 14

Spirillum

Question 15

Total magnification=

Answer 15

Objective power x 10

Question 16

Growth of cells of one specific microorganism

Answer 16

Pure culture

Question 17

A medium may be a

Answer 17

Liquid or soli

Question 18

Solid medium will have

Answer 18

Agar

Question 19

Must be heated to boiling to dissolve and will solidify at 40C

Answer 19

Agar

Question 20

Infection origination in a hospital

Answer 20

Nosocomial Infection

Question 21

_____ a slide of bacteria improves the contrast between the cells and the background

Answer 21

Staining

Question 22

Disadvantage of staining?

Answer 22

Drying and fixation of the cells onto a slide introduces some shrinkage and distortion to cell

Question 23

Will kill the cells and also ensure that they will stay attached tithe glass surface during staining

Answer 23

Fixation

Question 24

Distinguish between different organisms on the basis of staining reactions

Answer 24

Differential stains

Question 25

Most useful differential stain in microbiology

Answer 25

Gram stain

Question 26

Differentiates cells on te basis of cell wall composition

Answer 26

Gram stain

Question 27

No lipid in their cell wall Decolonize with ethanol or acetone Retain crystal violet Purple

Answer 27

Gram positive

Question 28

Decolonize and accept the counterstain | Appear pink

Answer 28

Gram negative

Question 29

What type of bacteria do you do acid stain for?

Answer 29

Mycobacterium Nocardia Staphylococcus

Question 30

Acid dye that stains background

Answer 30

Negative stain

Question 31

Used a modified negative stain with both the background and cells being stained

Answer 31

Capsule stain

Question 32

_____ remains unstained, giving the cells a halo appearance

Answer 32

Capsule stain

Question 33

Uses heat to penetrate the spore with the dye

Answer 33

Endospore stain

Question 34

All over flagella

Answer 34

Peritrichous

Question 35

Multiple flagella on one end

Answer 35

Polar lophotrichous

Question 36

1 flagella on one end

Answer 36

Polar monotrichous

Question 37

Flagella on both ends

Answer 37

Polar Amphitrichous

Question 38

Uses a needle

Answer 38

Motility test

Question 39

Indicated by growth away from the line of inoculation

Answer 39

Motility test

Question 40

Steps of motility test

Answer 40

Heat needle Obtain organism Stab straight down 1 time

Question 41

Enzymes that function outside the cell

Answer 41

Exoenzymes

Question 42

Enzymes that function inside the cell

Answer 42

Endoenzyme

Question 43

Collects gas produced during fermentation

Answer 43

Durham tube

Question 44

Used in the fermentation of lactose

Answer 44

Durham tube

Question 45

Anaerobic process

Answer 45

Fermentation of lactose

Question 46

Test tube that has a second inverted tube inside used to capture fermented gas

Answer 46

Durham tube

Question 47

One or more components that inhibit most microorganisms and allows a select group to grow

Answer 47

Selective media

Question 48

Contain indicators that allow the distinguishing of different organisms on the basis of their appearance on the media or the color of the media around the growth

Answer 48

Differential media

Question 49

Mannitol salt agar

Answer 49

Selective | Differential

Question 50

MacConkey's Agar

Answer 50

Selective | Differential

Question 51

Blood agar

Answer 51

Differential

Question 52

Lowenstein-Jensen

Answer 52

Selective

Question 53

Sabouraud's dextrose agar

Answer 53

Selective