Lecture 1 Flashcards
(27 cards)
Hematoxylin
4: RNA, DNA, ribosomes and rough ER
Color: blue
Eosin
5: secretory vesicles, soft ER, lysosomes, mitochondria,, and type I collagen
Color: pink
Feulgen reaction
DNA
Color: magenta
mallory triple
4: Nuclei (red), muscle (red/orange), collagen(blue), hyaline (transparent) cartilage (blue)
PAS reaction
Carbohydrates
color: Magenta
osmic acid
lipids
color: black
verhoeff
elastic fibers
color: black
silver methods
3: intermediate filaments of nerve cells, glial cells, reticular fibers (connective tissue fibers with type 3 collagen)
color: black
Trypan blue
macrophages
color: blue
pruissian blue
hemosiderin (ferric iron)
Color: blue
Nissel
Ribosomes
Color: blue
Iron Hematoxylin
4: Nuclear elements, chromosomes, mitochondria, centrioles, and muscle striation
Color: dark blue- back
What can eosin and hematoxylin be used for, clinically?
coagulative necrosis
what can PAS be used for, clinically?
To be used to thicken the basement membranes for kidney disease samples, glycogen storage diseases and in alpha antitrypsin deficiency in liver cells (hepatocytes)
what can mallory triple be used for, clinically?
to highlight fibrosis
what can feulgen be used for, clinically?
nuclear changes in cancer
what can prussian blue be used for, clinically?
demonstrate excessive iron in hemochromatosis
what can congo red be used for, clinically?
demonstrate extracellular deposits of amyloid
what can verhoeff be used for, clinically?
elastic fibers in marfan’s
what is immunohistochem? indirect vs direct method-
use of antibodies with markers to ID antigen, TO CATEGORIZE A TUMOR ORIGIN
indirect- more sensitive, more antibodies bind antigen
direct- less sensitive, antibodies bind less antigen
what is in situ hybridization?
uses a complementary nucleic acid strand to:
Identify a specific nucleic acid sequence in order to:
see amplifications, identify viruses, identify specific genes in a chromosome
what would you use to identify receptors on cells (like estrogen receptors in breast cancer or ERB B2)
immunohistochemistry
what would you use to find kaposi sarcoma d2-40?
immunohistochem
what would you use to find cytokeratins?
immunohistochem
