Lecture--Chapter 13 Flashcards Preview

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Flashcards in Lecture--Chapter 13 Deck (85)
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1

DNA strands are _____ and _____.

complementary; antiparallel

2

template strand specifies its complement

Chargaff's rule

3

DNA strands have a ____ phosphate end and ____ OH end, replication is _____.

5'; 3'; directional

4

The two DNA strands separate, with each strand used as a template for synthesis of _____.

daughter strands

5

Replication is _____.

semi-conservative

6

Steps of bacterial replication:

1. initiation
2. elongation
3. termination

7

Initiation of DNA replication starts at the ______.

origin of replication (oriC)

8

site where parental DNA strands have separated and new daughter strands are being made

replication fork

9

Elongation and ___ of new DNA.

synthesis

10

Replication is _____.

bidirectional

11

replication forks meet on opposite side of the circle and chromosomes separate

termination

12

motifs that bind proteins

sequence "boxes"

13

controls timing of replication

GATC methylation

14

Initiation of DNA replication: _____ initiators, separation of ____ region.

dnaA protein; AT-rich

15

Initiation of DNA replication: Binding of _____, establishing the _____.

helicase; 2 replication forks

16

Initiation of DNA replication: ____ and other proteins bind and unwind DNA.

DNA helicase

17

Initiation of DNA replication: ____ generates positive supercoiling ahead of each replication fork.

DNA helicase

18

Initiation of DNA replication: _____ travels ahead of the helicase and relieves positive supercoils.

DNA gyrase (topoisomerase II)

19

Initiation of DNA replication: ______ bind to the separated strands to keep them apart.

single-strand DNA binding proteins

20

enzymes that copy DNA

DNA polymerases

21

the primary polymerase of replication

DNA polymerase III (DNA Pol III)

22

has a role in "lagging strand" replication

DNA polymerase I (DNA Pol I)

23

DNA polymerases are:

1. primer dependent
2. directional
3. processive

24

DNA polymerases: only can add nucleotides to a primer

primer dependent

25

DNA polymerases: can only synthesise 5' to 3'

directional

26

DNA polymerases: remain on the strand and catalyse consecutive reactions

processive

27

___ enters catalytic site

dNTP

28

Base pairing according to _____ rule.

AT/GC

29

A phosphodiester bond forms between:

5'-P of entering dNTP and 3'-OH of the sugar of the previous deoxynucleotide

30

Outer 2 phosphates of a phosphodiester bond are ____.

released