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AUBF > M3: Analysis Automation (Part 3: Sediment/Cell Analyzers) > Flashcards

M3: Analysis Automation (Part 3: Sediment/Cell Analyzers) Flashcards

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1
Q

SEDIMENT/CELL ANALYZERS

  • This type of sediment/cell analyzers provides efficient standardized results
  • markedly improves TAT
A

Automated Microscopy Analyzers

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2
Q

SEDIMENT/CELL ANALYZERS

What are 2 types of technology used in sediment/cell analyzers

A
  • Flow cytometry
  • Digital imaging techniques
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3
Q

What are the automated urine system for sediments/cells?

A
  • Sysmex UF-1000i Fully Automated Urine Particle Analyzer and Sysmex UN-2000 Automated Urinalysis System
  • Beckman Coulterʼs iQ200 Microscopy Analyzer
  • 77 Elektronika UriSed 2 Automated Urine Sediment Analyzer
  • 77 Elektronika UriSed 3Pro Automated Urine Sediment Analyzer
  • FUS-100 and FUS-200 Sediment Analyzers
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4
Q

Automated urine system for sediments/cells

  • Uses fluorescent flow cytometry
  • Measures forward, side, and flourescent signal
  • Has 2 channels
  • uses 1.2 mL of uncentrifuged urine
  • particles are identified by measuring the height and width of the fluorescent and light scatter signals
A

Sysmex UF-1000i Fully Automated Urine Particle Analyzer and Sysmex UN-2000 Automated Urinalysis System

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5
Q

Automated urine system for sediments/cells

In Sysmex UF-1000 and Sysmex UN-2000, it uses flow cytometry to measure what (3) parameters of light

A
  1. Forward light scatter
  2. Side scatter
  3. Fluorescent signal
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6
Q

Automated urine system for sediments/cells

This measures size (length of cells)

Sysmex UF-1000 and Sysmex UN-2000

(3) parameters of light

A

Forward light scatter

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7
Q

Automated urine system for sediments/cells

Measures shape and internal structure/complexity of the element

Sysmex UF-1000 and Sysmex UN-2000

(3) parameters of light

A

Side scatter

Sysmex UR-1000 and Sysmex UN-2000

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8
Q

Automated urine system for sediments/cells

Measures cellular inclusions

Sysmex UF-1000 and Sysmex UN-2000

(3) parameters of light

A

Fluorescent signal

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9
Q

Automated urine system for sediments/cells

What are the requirements in Sysmex UF-1000i and Sysmex UN-2000?

Sysmex UF-1000 and Sysmex UN-2000

A
  • 1.2 mL of uncentrifuged urine
  • 2 channels (sediment and bacterial channel)
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10
Q

Automated urine system for sediments/cells

T or F

1.2 mL of centrifuged urine is used in Sysmex UF-1000i and Sysmex UN-2000

Sysmex UF-1000 and Sysmex UN-2000

A

F (1.2 mL uncentrifuged urine)

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11
Q

Automated urine system for sediments/cells

Channel for urine particle analysis

Sysmex UF-1000 and Sysmex UN-2000

A

Sediment channel

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12
Q

Automated urine system for sediments/cells

Channel for bacteria staining and detection

Sysmex UF-1000 and Sysmex UN-2000

A

bacterial channel

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13
Q

Automated urine system for sediments/cells

This stabilizes the pH and lyses the non-bacterial particles

Sysmex UF-1000 and Sysmex UN-2000

bacteria channel

A

diluent

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14
Q

Automated urine system for sediments/cells

T or F

stain in bacterial channel is specific to the RNA in a bacterial cell

Sysmex UF-1000 and Sysmex UN-2000

A

T

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15
Q

Automated urine system for sediments/cells

Bacterial channel is incubated at what temperature?

Sysmex UF-1000 and Sysmex UN-2000

A

42 c

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16
Q

Automated urine system for sediments/cells

Resulting values of Sysmex UF-1000 and Sysmex UN-2000 are presented in what unit?

Sysmex UF-1000 and Sysmex UN-2000

A

quantitative cells per microliter (μ) and cells per HPF or LPF

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17
Q

Automated urine system for sediments/cells

Main particles detected in Sysmex UF-1000 and Sysmex UN-2000

Sysmex UF-1000 and Sysmex UN-2000

A
  • RBCs
  • WBC
  • SECs
  • Hyaline casts
  • Bactera
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18
Q

Automated urine system for sediments/cells

Flagged particles in Sysmex UF-1000 and Sysmex UN-2000

Sysmex UF-1000 and Sysmex UN-2000

A
  • Pathologic casts
  • Crystals
  • Small round cells (RTE/transitional ECs), sperm, mucus, yeast-like cells
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19
Q

Automated urine system for sediments/cells

Identify if main particles detected or flagged particle:
Pathologic casts

Sysmex UF-1000 and Sysmex UN-2000

A

flagged particles

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20
Q

Automated urine system for sediments/cells

Identify if main particles detected or flagged particle:
Crystals

Sysmex UF-1000 and Sysmex UN-2000

A

flagged particles

Sysmex UF-1000 and Sysmex UN-2000

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21
Q

Automated urine system for sediments/cells

Identify if main particles detected or flagged particle:
RTE cells

Sysmex UF-1000 and Sysmex UN-2000

A

flagged particle

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22
Q

Automated urine system for sediments/cells

Identify if main particles detected or flagged particle:
Transitional ECs

Sysmex UF-1000 and Sysmex UN-2000

A

flagged particles

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23
Q

Automated urine system for sediments/cells

Identify if main particles detected or flagged particle:
sperm

Sysmex UF-1000 and Sysmex UN-2000

A

flagged

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24
Q

Automated urine system for sediments/cells

Identify if main particles detected or flagged particle:
mucus

Sysmex UF-1000 and Sysmex UN-2000

A

flagged

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25
# Automated urine system for sediments/cells Identify if main particles detected or flagged particle: yeast-like cells | Sysmex UF-1000 and Sysmex UN-2000
flagged | Sysmex UF-1000 and Sysmex UN-2000
26
# Automated urine system for sediments/cells Identify if main particles detected or flagged particle: RBCs | Sysmex UF-1000 and Sysmex UN-2000
main particle
27
# Automated urine system for sediments/cells Identify if main particles detected or flagged particle: WBCs | Sysmex UF-1000 and Sysmex UN-2000
main particle
28
# Automated urine system for sediments/cells Identify if main particles detected or flagged particle: SECs | Sysmex UF-1000 and Sysmex UN-2000
main particle
29
# Automated urine system for sediments/cells Identify if main particles detected or flagged particle: Hyaline casts | Sysmex UF-1000 and Sysmex UN-2000
main particle
30
# Automated urine system for sediments/cells Identify if main particles detected or flagged particle: Bacteria | Sysmex UF-1000 and Sysmex UN-2000
main particle
31
# Automated urine system for sediments/cells Flagged particles indicate the need to perform what (2) procedures? | Sysmex UF-1000 and Sysmex UN-2000
Manual microscopic analysis and identification
32
# Automated urine system for sediments/cells T or F Sysmex UF-1000 and Sysmex UN-2000 can store up to 10,000 results including scattergrams, histograms, and specimen characteristics | Sysmex UF-1000 and Sysmex UN-2000
T
33
# Automated urine system for sediments/cells T or F Sysmex UF-1000 and Sysmex UN-2000 can only be used alone | Sysmex UF-1000 and Sysmex UN-2000
F (independently used or integrated with automated urine chemistry strip reader to create a complete urinalysis)
34
* the principle also used for CBC (specifically, RBC, WBC, and platelet counts) * the passage of different urine elements in a single file in front of a laser so they can be detected, counted, and sorted
Flow cytometry
35
Components of a flow cytometry
* Laser * Fluidic system * Optic system * Photodetector * Computer system
36
# Flow cytometry Process of flow cytometry
1. Some elements are fluorescently-labelled and excited by the **laser** (emit light at varying wavelengths) 2. The **optic system** gathers the light 3. **Detector** receives light which converts the received scattered light into a voltage pulse 4. The **computer system** converts/transcribes the data into a histogram or scattergram
37
# Components of a flow cytometry Gathers light
Optic system
38
# Components of a flow cytometry Converts the received scattered light into a voltage pulse
detector
39
# Components of a flow cytometry Converts/transcribes the data into a histogram or scattergram
Computer system
40
# Components of a flow cytometry Flow cytometry can transcirbe data into a. histogram b. scattergram c. both d. NOTA
c. both
41
# Automated urine system for sediments/cells * uses **digital flow morphology** (imaging) and **auto particle recognition (APR)** to categorize and count urine particles automatically in uncentrifuged urine based on size, shape, texture, and contrast * uses 1.3 mL of urine * Used for body fluid cells * Digital camera takes 500 images
Beckman Coulterʼs iQ200 Microscopy Analyzer
42
# Automated urine system for sediments/cells T or F Beckman Coulterʼs iQ200 Microscopy Analyzer is not used ofr body fluid cells
F (also used)
43
# Automated urine system for sediments/cells T or F The sample moves in planar flow cell and presented to a digital microscope in a thick moving sheet | Beckman Coulterʼs iQ200 Microscopy Analyzer
F (sample moves in planar flow cell and presented to a digital microscope in a **thin moving sheet**)
44
# Automated urine system for sediments/cells Volume of urine used? (centrifuged or uncentrifuged?) | Beckman Coulterʼs iQ200 Microscopy Analyzer
1.3 mL of uncentrifuged urine
45
# Automated urine system for sediments/cells * Digital camera takes how many images as sample advances through flow cell | Beckman Coulterʼs iQ200 Microscopy Analyzer
500 images
46
# Automated urine system for sediments/cells What sediments does Beckman Coulterʼs iQ200 Microscopy Analyzer classify? | Beckman Coulterʼs iQ200 Microscopy Analyzer
RBCs, WBCs, WBC clumps, SECs, non-SECs, unclassified casts, hyaline casts, unclassified crystals, bacteria, yeast, mucus, and sperm | subclassifications also exist in 27 more categories
47
# Automated urine system for sediments/cells * performs **automated microscopy with digital imaging** using **auto image evaluation module** (AIEM) software * Uses 2mL of centrifuged urine, bright field microscope * Analyzed by bright-field microscope * a digital camera captures and categorizes 15 particle images based upon size and shape using AIEM software * Zoom capability to view images
77 Elektronika UriSed 2 Automated Urine Sediment Analyzer
48
# Automated urine system for sediments/cells T or F 77 Elektronika UriSed 2 Automated Urine Sediment Analyzer's interpretation is like that of automatic microscopic smears
F (interpretation is like that of **manual microscopic smears**)
49
# Automated urine system for sediments/cells * incorporates **phase contrast microscopy** in addition to bright-field microscopy to improve differentiation of elements such as hyaline casts, RBC membranes, crystals, and yeasts
77 Elektronika UriSed 3Pro Automated Urine Sediment Analyzer
50
# Automated urine system for sediments/cells What can be linked with 77 elektronika urised 2 and 3pro to make complete automated urinalysis laboratory system
LabUMat 2 | LAB U
51
# Automated urine system for sediments/cells * **flat-flow cell digital imagine technology** * artificial imaging identification (AII) software classifies and quantifies the cells and formed particles in the uncentrifuged urine * a digital camera captures up to 820 photos
FUS-100 and FUS-200 Sediment Analyzers
52
# Automated urine system for sediments/cells classifies and quantifies the cells and formed particles in the uncentrifuged urine FUS-100 and FUS-200
artificial imaging identification (AII) softwarre
53
# Automated urine system for sediments/cells Centrifuged or uncentrifuged urine? | FUS-100 and FUS-200 Sediment Analyzers
uncentrifuged urine
54
# Automated urine system for sediments/cells digital camera captures to how many photos? | FUS-100 and FUS-200 Sediment Analyzers
820 photos ## Footnote it identifies and classifies 12 visible components in urine
55
# Automated urine system for sediments/cells How many visible components in urine are identified and classified? | FUS-100 and FUS-200 Sediment Analyzers
12
56
# Automated urine system for sediments/cells What can be integrated with FUS-100 and FUS-200 Sediment Analyzers for a fully automated urinalysis system | FUS-100 and FUS-200 Sediment Analyzers
H-800 chemistry analyzer
57
Zoom capability a. Beckman's Coluter iQ200 microscopy analyzer b. Sysmex UF-1000 and Sysmex UN-2000 automated urinalysis system c. both d. NOTA
d. NOTA ## Footnote 77 ELEKTRONIKA URISED 2 AUTOMATED URINE SEDIMENT IS THE CORRECT ANSWER
58
Fluorescent flow cytometry a. Sysmex UF-1000 and Sysmex UN-2000 b. Beckman's Coulter iq200 microscopy analyzer c. both d. NOTA
a. Sysmex UF-1000 and Sysmex UN-2000
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1.3 mL of centrifuged urine a. Beckman's Coulter iq200 microscopy analyzer b. 77 Elektronika UriSed 2 Automated Urine Sediment Analyzer c. both d. NOTA
d. NOTA | BECKMAN SHOULD BE 1.3 mL UNCENTRIFUGED URINE
60
Digital camera a. Beckman's Coulter iq200 microscopy analyzer b. FUS-100 and FUS-200 Sediment Analyzers c. both d. NOTA
c. both
61
Phase-contrast microscope a. 77 Elektronika UriSed 2 Automated Urine Sediment Analyzer b. 77 Elektronika UriSed 3Pro Automated Urine Sediment Analyzer c. both d. nOTA
b. 77 Elektronika UriSed 3Pro Automated Urine Sediment Analyzer
62
Centrifuged urine a. 77 Elektronika UriSed 2 Automated Urine Sediment Analyzer b. 77 Elektronika UriSed 3Pro Automated Urine Sediment Analyzer c. both d. nOTA
c. both
63
Uncentrifuged urine a. Sysmex UF-1000i and Sysmex UN-2000 Automated Urinalysis System b. Beckman Coulterʼs iQ200 Microscopy Analyzer c. FUS-100 and FUS-200 Sediment Analyzers d. ALL e. NOTA
d. ALL
64
# Automated urine system for sediments/cells How many particle images does 77 Elektronika UriSed 2 Automated Urine Sediment Analyzer capture and categorize? | 77 Elektronika UriSed 2 Automated Urine Sediment Analyzer
15 particle images | using AIEM software

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