Micro Exam 1c Flashcards
(25 cards)
hyperthermophiles
65 to 110 celcius
thermophiles
40 to 72 celcius
mesophiles
10 to 50 celcius
psychrotrophs
0 to 30 celcius
psychrophiles
-10 to 20 celcius
physical requirements for growth
- temp
- pH
- osmotic pressure
TOP
chemical requirements for growth
- carbon
- nitrogen, sulfur, phosphorus
- trace elements
- oxygen
- organic growth factor
at what pH do most bacteria grow
6.5-7.5
plasmolysis
hypertonic solutions, or increase in salt or sugar
extreme or obligate halophiles
require high osmotic pressures
facultative halophiles
tolerate high osmotic pressure
microaerophilic
grow best in pure oxygen
faculative anaerobic
grow under semi-anaerobic but prefer aerobic
aerotolerant anaerobic
anaerobic that can tolerate oxygen
obligate anaerobic
need to function in no oxygen
sulfur
used in amino acids, thiamine and biotin
phosphorus
in DNA, RNA, ATP and membranes
toxic oxygen
- singlet oxygen
- superoxide free radical
- peroxide anion
- hydroxyl radical
chemically defined media
is one in which the exact chemical composition is known
complex media
is one in which exact chemical composition varies slightly from batch to batch
reducing media
growth of obligate anaerobes
selective media
encouraging desired microbe growth
differential media
make it easy to distinguish colonies of different microbes
enrichment media
increase number of desired microbes
