Microscope

Question 1

who did the earliest records of microscopic observations

Answer 1

anton van leeuwenhoek

Question 2

what did anton van leeuwenhoek first observe and what type of microscope did he use

Answer 2

protozoans - simple microscope

Question 3

how did early microscopes first start out and where

Answer 3

lenses in tubes - holland

Question 4

Magnification vs Linear Magnification

Answer 4

Magnification: magnitude of amplification of image by the microscope

Linear Magnification: magnitude of amplification of image by the objectives lens only

Question 5

2 types of magnification

Answer 5

Theoretical (ocular lens x objective lens)

Actual (apparent size/actual size)

Question 6

Resolving Power vs Limit of resolution

Answer 6

Resolving Power - ability of a microscope to distinguish between two adjacent points as DISTINCT and SEPARATE

Limit of Resolution - smallest distance that can be distinguished by microscope

Question 7

An __ of the ___-gathering ___ of the ___ lens

Answer 7

index
light
power
objective

NUMERICAL APERTURE

Question 8

1. RELATIONSHIP between NA and LIMIT OF RESOLUTION
2. NA of each objective lens

Answer 8

1. inversely proportional

LPO - 0.25
HPO - 0.65
OIO - 1.30

Question 9

The ability of medium to deflect light

and what does a high value of it indicates

Answer 9

refractive index

High RI - less deflection - more light

Question 10

RI of water and air

Answer 10

air - 1.00
water - 1.56

Question 11

Working distance vs Field of view

+ value per objective lens

Answer 11

WD - distance between the specimen and objective lens
LPO: 5
HPO: 0.46
OIO: 0.13

FV - area visible under microscope
LPO: 1.50
HPO. 0.35
OIO: 0.18

Question 12

___ OF FOCUS definition

Answer 12

depth

• property that indicates how deep the space the microscope can bring into clear view

Question 13

APERTURE DISC VS EXTENSION TUBE

Answer 13

AD- under stage and contains holes w/ diff sizes to regulate light

ET - below eyepiece and also contains lens

Question 14

How many inches should a microscope be placed far from a table’s edge

Answer 14

2 inches

Question 15

what to use to clean the lenses

Answer 15

lens paper

Question 16

How to know if the EYEPIECE or the OBJECTIVE LENS are dirty?

Answer 16

eyepiece- dirt rotates with eye piece

objective lens - if dirt does not rotate (also check slide, mirror and condenser)

Question 17

what position should the microscope be in when observing WET MOUNTS

Answer 17

upright

Question 18

can u use alcohol on microscopes

Answer 18

no

Question 19

can u focus an HPO downwards using objective lens

Answer 19

no