Microscopic Examination of Urine - RBC, WBC, and Epithelial Cells Flashcards

1
Q

Crenated RBCs are seen in urine that is:

A

Hypertonic

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2
Q

“Ghosts” or swollen RBCs are seen in urine that is:

A

Hypotonic

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3
Q

How many RBCs are seen in normal urine?

A

0-2/hpf

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4
Q

What are two reasons for a positive chem strip, but no visible RBCs in the urine?

A
  1. Blood is alkaline.

2. Hypotonic urine. Check specific gravity.

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5
Q

What are two reasons for a negative chem strip, but visible RBCs in urine?

A
  1. Ascorbic acid.

2. Check for yeast or calcium oxalate crystals.

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6
Q

RBCs in urine indicates:

A
  • Patient is in early renal disease.
  • Dysomorphic = diseased glomerulus.
  • Can be a contaminant.
  • Can be caused by smoking and excessive exercise.
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7
Q

RBCs can be mistaking for:

A
  • WBCs.
  • Yeast.
  • Round from of calcium oxalate.
  • Oil droplets.
  • Air bubbles.
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8
Q

What is the physical appearance of neutrophils?

A
  • Larger than red blood cells.
  • Vary in size with tonicity.
  • Grainy cytoplasm and segmented nuclei.
  • Grayish single or clumps (report clumps).
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9
Q

What is the physical appearance of aging neutrophils?

A
  • Nuclear lobes fuse.
  • Swell and may lyse.
  • “Glitter cells”.
  • Cytoplasma forms granular blebs or filaments.
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10
Q

What is the total number of neutrophils in hpf?

A

0-5 or 0-8.

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11
Q

During physical examination of the urine, how will neutrophils make the urine look?

A
  • Cloudy.
  • Foul oder.
  • macroscopic white button.
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12
Q

Why would a reagent strip test positive for neutrophils (WBC), but be negative under the microscope?

A

Lysis

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13
Q

Why would a reagent strip test negative for neutrophils (WBC), but be negative under the microscope?

A
  • Other types of cells.
  • Check test strip function.
  • Not enough cells to cause reaction.
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14
Q

Neutrophils look like:

A
  • Tubular epithelial cells.
  • Red bleed cells.
  • Trichomonads and fungi.
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15
Q

What is used to differentiate neutrophils from renal tubular epithelial cells?

A
  • 2% acetic acid.

- 0.5% toluidine blue.

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16
Q

Neutrophils is urine can be a clinical significance for?

A
  • Renal diseases.
  • Inflammatory conditions.
  • Bacterial.
  • Nonbacterial.
17
Q

Eosinophils can be differentiated from neutrophils with what stain?

A

Hansel

18
Q

Why would you need to use the Hansel stain?

A

To differentiate eosinophils from neutrophils.

19
Q

What conditions would the presence of eosinophils in urine indicate?

A
  • Drug induced acute interstitial nephritis.
  • Rare chronic UTI.
  • Rejection of transplants.
20
Q

WBC in urine is only significant at what levels?

A

Greater than 1%.

21
Q

Clinical significance of lymphocytes in the urine?

A

Rejection of transplants.

22
Q

What is done if you see clumps of white blood cells?

A

Report them. They aren’t good for patient.

23
Q

Which cells line the entire urinary tract?

A

Epithelial cells.

24
Q

What is the most common and largest epithelial cell?

A

Squamous

25
Q

Where are the squamous cells located?

A

The entire female urethra and the distal portion of male urethra.

26
Q

Which cells are described as flag-stone?

A

Squamous

27
Q

When are squamous cells considered an issue?

A
  • When they are covered with bacteria.

- Usually a vaginal infection with Gardnerella vaginalis.

28
Q

Where are the transitional epithelial cells located?

A

Renal calyces to bladder and male urethra.

29
Q

What could be the cause of large amounts of renal tubular epithelial cells?

A
  • Acute tubular necrosis.
  • Heavy metal poisoning.
  • Viral infections.
  • Renal transplant rejection.
30
Q

How many renal tubular epithelial cells are normal at hpf?

A

15

31
Q

How can you verify it is a renal tubular epithelial cell?

A
  • Polarizing scope.

- Fat stains.