microscopy

Question 1

magnification

Answer 1

make small stuff look bigger

Question 2

resolution

Answer 2

ability to tell 2 objects from eachother

Question 3

contrast

Answer 3

difference in light intensity between image & background

Question 4

what do scientists use when the contrast is low and they cant see much?
what are some common types

Answer 4

-stains
- methylene blue, iodine

Question 5

compound light microscope

Answer 5

• 2+ lenses
• uses light

Question 6

objective lens

Answer 6

closer to object (4x, 10x, 40x)

Question 7

ocular lens

Answer 7

closer to eye
aka eyepiece
(10x)

Question 8

fluorescent microscopy

Answer 8

-fluorescence = ability of certain chemicals to give visible light after absorbing radiation which is not normally visible, such as ultraviolet light.
-in microscopy fluorescent substances added to cells to see them better

Question 9

electron microscopes

Answer 9

• uses beam of electrons instead of light
  1. transmission electron microscope (TEM)
  2. scanning electron microscope

Question 10

Transmission Electron Microscope (TEM)

Answer 10

-compresses NON-LIVING cells with heavy metals on top and shoots beam of electrons through
-x1,500,000 zoom

Question 11

Scanning Electron Microscope

Answer 11

• shoots beam over & across sample
• 3D view of some LIVING & NON-LIVING samples
  -x300,000 zoom