Module 3 Flashcards

Biology of the Microbial Cell

1
Q

Spontaneous generation

A

life arises from non living matter

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2
Q

Francesco Redi

A

provided experimental data against spontaneous generation

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3
Q

Louis Pasteur

A

proved life only comes from life using the swan neck flask

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4
Q

Robert Hooke

A

observed and coined the term cells

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5
Q

7 statements of cell theory

A

living are made from one or more cells
fundamental components of life
arise from pre existing cells
organism activity depends on cell activity
energy flow occurs within a cell
cells contain DNA and RNA
similar species have similar cellular compositions

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6
Q

who was the endosymbiotic theory proposed and revived by

A

konstantin mereschkowski
lynn margulis

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7
Q

what does the endosymbiotic theory state

A

fusion of prokaryotes with ancestral bacteria gave prokaryotes mitochondria

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8
Q

prokaryotes include what two groups
what is the defining feature

A

bacteria and archaea
not inclosed nucleus

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9
Q

coccus

A

round

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10
Q

bacillus

A

rod

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11
Q

vibro

A

curved rod

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12
Q

coccobacillus

A

short rod

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13
Q

spirillum

A

spiral

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14
Q

spirochete

A

long loose spiral

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15
Q

diplo-

A

pair

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16
Q

tetra-

A

4 in a square

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17
Q

strepto-

A

chain

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18
Q

staphylo-

A

cluster

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19
Q

inclusions and compartments (2)

A

in prokaryotes
condensed areas of energy, gas

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20
Q

Endospore (3)

A

in gram positive
not metabolically active therefore resistant to killing
spores form inside the cell then lyse out for germination

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21
Q

cell envelope

A

layers around the cytoplasm with diverse functions

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22
Q

Bacteria and Eukarya membrane (3)

A

phospholipid bilayer
tail and head
ester linkages

23
Q

archaea membrane (3)

A

bilayer or monolayer
sidechains
ether linkages

24
Q

simple diffusion (4)

A

small and uncharged molecules
linear rate of diffusion (no saturation point)
inefficient for bacteria
ex)glycerol, h2o, o2

25
facilitated diffusion (3)
concentration gradient dependent more efficient limited based on channel properties
26
active transport (3)
energy to move against the concentration gradient carrier proteins used can be saturated
27
coupled active transport (2)
antiport or symport uses proton motor force and concentration gradients
28
ABC transporters
must have protein bound to a substrate to enter the cell
29
Group translocation (2)
phosphate transfer allows for sugar transport energy from high energy phosphates
30
bacterial cell walls provide (2)
rigidity to maintain cell shape prevent osmotic lysis
31
peptidoglycan layer when it comes to gram bacteria
positive thick negative thin + outer membrane
32
why is peptidoglycan layer missing in gram negative bacteria
missing pentapeptide sugar
33
sugars in bacteria
NAG and NAM
34
sugars in archaea what is special?
NAG and NAT making pseudomurin which is resistant to lysozyme
35
Eukaryote cell wall composition options
cellulose or chitin
36
lysozymes
defense mechanisms in animals which target bacteria due to their cell wall sugar composition
37
lipopolysaccharides
in the envelope around gram negative bacteria only provide attachment, mechanical strength, immune invasion, and protection endotoxin causing spesis
38
Deep branching bacteria
stain gram negative but do not have LPS so are not truley gram negative
39
S Layer (3)
protein mesh layer on archaea and some bacteria archaea: cell wall, protects against osmotic lysis, cell shape bacteria: protection and adhesion
40
capsule/slime layer (2)
sticky polysaccharide layer attachment, biofilm, protection, adhesion
41
capsule
tight matrix strongly attached to the cell
42
slime layer
easily deformed and loosely attached
43
outermost layer of cell options (3)
s layer capsule slime layer
44
pili vs fimbrae
pili are thin filaments with multiple functions while fimbrae are short pili used for attachment both play roles in conjugation, twithcing motility, and pathogenicity
45
flagellum (6)
thin protein appendages rotational speeds of 1000 rpm clockwise rotation = tumbling counterclockwise rotation = running made of flagellin build at tip using proton motor force
46
archaellum
smaller than flagella solid not hollow build at base by ATP
47
twitching (3)
driven by ATP type 4 pili extend then pull
48
gliding (3)
proton motor force smooth and continuous no external propulsion
49
taxis
description of movement
50
chemotaxis can be observed using
darkfield microscopy or pipette and plate
51
different types of taxis (5)
osmo hydro photo aero magneto
52
eukaryotic motility includes
swimming flexile whip
53
swimming
due to long flagella and short cilia
54
flexile whip
microtubules in the cytoplasmic membrane powered by ATP