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Histopath Lec > Mounting and labeling > Flashcards

Mounting and labeling Flashcards

1
Q

last step in tissue processing that results in a
permanent histological preparation suitable for
microscopy, after adhesion of the sections on to
the slide and appropriate staining of the tissue.

A

Mounting

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2
Q

usually a syrupy fluid applied between the section and the
coverslip after staining, setting the section firmly,
preventing the movement of the coverslip

A

Mounting medium

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3
Q

important because it governs the contrast between the
cellular detail and the background, and also the
transparency of the observed sample against the bright
field of the microscope

A

Refractive index

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4
Q

Mounting media may be divided
into two main groups:

A
  • Aqueous Media
  • Resinous Media
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5
Q

used for mounting
sections from distilled water when the stains would be
decolorized or removed by alcohol and xylene

A

Aqueous mounting medium

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6
Q

Aqueous mounting medium is made up of

A

made up of gelatin, glycerin jelly or gum arabic, glycerol, sugar, and a preservative solution.

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7
Q

Purpose of glycerin jelly or gum arabic

A

to
solidify the medium

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8
Q

Purpose of glycerol

A

to prevent cracking and
drying of the preparation

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9
Q

Purpose of sugar

A

to increase the
refractive-index

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10
Q

has a low refractive index, is moderately transparent and
evaporates easily, hence is good only for temporary
mounting.

A

Water

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11
Q

improves the image
quality and also supports the specimen.

A

Refractive index of water

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12
Q

Why wet mounts cannot be stored over extended time period

A

water will evaporate

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13
Q

has a high index of refraction and provides greater
visibility if slightly diluted with water

A

Glycerin

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14
Q

Glycerin refractive index

A

1.46

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15
Q

miscible with water, is inexpensive, and is non-poisonous.

A

Glycerin

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16
Q

Disadvantage of glycerin

A
  • difficult to prepare slides that are truly permanent in
    nature.
  • slowly run off a slide that is tilted.
  • Do not stack slides for long as the pressure will squeeze
    glycerin from the mounts.
  • Glycerin will eventually evaporate and air will penetrate
    under the coverslip.
  • Glycerin can be attacked by microorganisms,
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17
Q

very suitable semi-permanent mounting medium

A

Glycerin

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18
Q

Commonly used to mount sections for immunofluorescence and glycerol may be added to other
agents to retard drying and cracking.

A

Phosphate buffered glycerol

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19
Q

Refractive index of Phosphate buffered glycerol

A

1.47

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20
Q

Formula of GLYCERIN JELLY (KAISER’S
1880)

A
  • Gelatin 10 gm.
  • Glycerol 70 ml.
  • Distilled water 60 ml.
  • Phenol crystals (preservative) 0.25 gm.
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21
Q

standard mounting medium used
when dehydration and clearing with xylene cannot be
made (as in fat stains)

A

Glycerin jelly

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22
Q

Glycerin jelly refractive index

A

1.47

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23
Q

FARRANT’S MEDIUM formula

A
  • Gum arabic 50 gm.
  • Distilled water 50 ml.
  • Glycerol 50 ml.
  • Sodium merthiolate 0.025 gm
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24
Q

FARRANT’S MEDIUM. Refractive index

25
Advantage of farrant's medium
does not solidify upon storage and therefore does not need to be heated before use.
26
Disadvantage of farrant's medium
takes a longer time to harden and may therefore require ringing
27
Arsenic trioxide may be used as a substitute of sodium merthiolate for preservation of the medium.
Farrant's medium
28
APATHY'S MEDIUM formula
* Pure gum arabic (crystals not powder) 50 gm. * Pure cane sugar or sucrose 50 gm. * Distilled water 50 ml. * Thymol crystals 0.05 gm.
29
Apathy's medium refractive index
1.52
30
used for methylene blue-stained nerve preparations and as a general purpose aqueous mountant.
Apathy's medium
31
Advantage of apathy's medium
one of the most useful aqueous mountants for fluorescent microscopy, being virtually non- fluorescent.
32
Disadvantage of apathy's medium
sets quite hard, has a higher refractive index, and does not require ringing.
33
recommended for mounting frozen sections from water.
BRUN'S FLUID
34
BRUN'S FLUID formula
* Glucose 24 gm. * Glycerine 6 ml. * Spirits of camphor 6 ml. * Distilled water 84 ml.
35
used for preparations that have been dehydrated and cleared in xylene or toluene, and are recommended for majority of staining methods.
RESINOUS MOUNTING MEDIA
36
RESINOUS MOUNTING MEDIA is divided into 2
natural and synthetic resins.
37
most important synthetic resins are used for
embedding undecalcified bones, and for electron microscopy
38
It is a transparent, almost colorless oleoresin that adheres firmly to glass and sets to a hard consistency without granulation.
Canada Balsam
39
Canada Balsam refractive index
1.524
40
recommended for whole mounts and for thick sections because it does not shrink much.
Canada balsam
41
may be added to Canada balsam to maintain its neutral reaction
Calcium carbonate chips
42
dissolved in xylene in an incubator at 37°C or paraffin oven at 58 °C
Canada balsam
43
Canadian tree:
Abus Balsamea
44
recommended for small tissue sections but not for whole mounts because of shrinkage produced on drying
DPX - (Dibutyl Phthalate and Xylene)
45
Disadvantage of DPX - (Dibutyl Phthalate and Xylene)
It tends to set quickly and often retract from the edge of the coverslip.
46
colorless, neutral medium in which most standard stains are well preserved.
DPX - (Dibutyl Phthalate and Xylene)
47
DPX - (Dibutyl Phthalate and Xylene) refractive index
1.532
48
synthetic resin mixture in xylene, available in a pale yellow or colorless solution
XAM
49
Xam refractive index
1.52
50
Dries quickly without retraction, and preserves stains well.
Xam
51
CLARITE refractive index
1.544
52
synthetic resin which is soluble in xylene generally preferred over D.P.X
CLARITE
53
Other recommended synthetic mounting media
* Permount (made by Fisher Scientific) * H.S.R. (Harleco Synthetic Resin) * Clearmount (Gurr)
54
process of sealing the margins of the cover-slip to prevent the escape of fluid or semi-fluid mounts and evaporation of mountant, to fix the coverslip in place, and to prevent sticking of the slides upon storage.
Ringing
55
The term “ringing” originated because
round coverslips were initially used and the coating applied in the form of a circle or “ring.”
56
made up of two parts paraffin wax mixed with 4-9 parts powdered colophonium resin, heated and filtered.
Kronig cement
57
cellulose adhesives
Durofix
58
often used as a mountant in immunofluorescence microscopy, has been recommended as an alternative for glycerin jelly
Polyvinyl alcohol

Histopath Lec (11 decks)

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