NHPs

Question 1

Temperature range for NHPs

Answer 1

64-84F

Question 2

Minimum relative humidity for tamarins and marmosets

Answer 2

50%

Question 3

Vaccines typically given to NHPs

Answer 3

Rabies, tetanus +/- MMR

Question 4

Most common site for blood collection from NHPs

Answer 4

Femoral vein

Question 5

Safe amount of blood to draw from an NHP

Answer 5

No more than 10-15% of total blood volume per 2 weeks

Question 6

Maximum volume of SC administration in NHPs

Answer 6

5 ml/kg/site

Question 7

Insertion of NG tubes in OWM vs NWM

Answer 7

OWM: Downward orientation of nares –> Upward insertion

NWM: Laterally flared nares –> Lateral insertion

Question 8

What are vero cells and from what species do they originate?

Answer 8

Kidney epithelial cell line from Chlorocebus (African green)

Question 9

What is MPTP used to induce?

Answer 9

Parkinson’s disease

Question 10

What is MOG and what is it used for?

Answer 10

Myelin oligodendrocyte glycoprotein

Induces EAE

Question 11

What is S. oedipus commonly used to study?

Answer 11

Cotton top tamarin

Chronic colitis and colon adenocarcinoma

Question 12

What NHP species are CITES I?

Answer 12

S. oedipus (cotton top tamarin), Saimiri oerstedii (black crowned squirrel monkey), Macaca silenus (lion tailed macaque), Pan

Question 13

What species is used to study baldness?

Answer 13

Stump tailed macaque (M. arctoides)

Question 14

What species are used in the MPTP induced model of Parkinson’s dz?

Answer 14

C. jacchus (marmoset), Chlorocebus aethiops (African green), M. mulatta (Rhesus), Pig-tailed macaque (M. nemestrina)

Question 15

What species are used as an EAE model? What is used to induce it?

Answer 15

Myelin oligodendrocyte glycoprotein (MOG)

C. jacchus (marmoset), M. mulatta (Rhesus), M. fascicularis (cyno)

Question 16

What species is used to study pelvic organ prolapse?

Answer 16

Saimiri spp. (Squirrel monkey)

Question 17

What species are used to study cardiomyopathy?

Answer 17

Saimiri spp. (squirrel monkey) and Sanguinus mystax (mustached tamarin)

Question 18

What enteric bacterial infection also causes gingivitis?

Answer 18

Shigella

Question 19

A macaque presents with mucohemorrhagic diarrhea. Three weeks later, he develops lameness on one limb. What is the agent?

Answer 19

Shigella

Question 20

What species of Helicobacter is associated with colonic adenocarcinoma in macaques?

Answer 20

H. macacae

Question 21

What agent causes fatal proliferative enteritis in juvenile rhesus?

Answer 21

Lawsonia intracellularis

Question 22

What is the clinical manifestation of S. pneumoniae in NHPs?

Answer 22

Pneumonia, meningitis, rapidly progresses to death

Low morbidity, high mortality

Question 23

What does Klebsiella pneumoniae cause in NHPs?

Answer 23

Pneumonia, meningitis, air sacculitis

Young more commonly affected

Question 24

What histopathology findings are characteristic of nocardia infection?

Answer 24

Pyogranulomas with sulfur granules and large colonies of filamentous bacteria

Question 25

What bacteria causes epistaxis in cynos, especially in dry months? How is it treated?

Answer 25

Moraxella catarrhalis Long-acting penicillin

Question 26

What are the differential diagnoses for cavitary lesions in the lungs?

Answer 26

Nocardia, TB, crypto, coccidiosis, Yersinia

Question 27

What is the gold standard diagnostic for tuberculosis?

Answer 27

Culture

Question 28

What bacterium is associated with acute gastric dilation in NHPs?

Answer 28

C. perfringens

Question 29

What is the most common opportunistic infection of macaques with SIV?

Answer 29

Pneumocystis

Question 30

Stain needed to visualize pneumocystis?

Answer 30

Silver or PAS

Question 31

Where in the country is Coccidioides immitis found?

Answer 31

Southwest US

Question 32

What are the characteristic lesions of Coccidioidmycosis?

Answer 32

Lesions in the lungs and vertebrae (cavitary lung lesions and/or vertebral bone lysis)

Question 33

What clinical signs does HIstoplasma capsulatum cause? Dx?

Answer 33

Disseminated microgranulomas confined to the skin GMS stain - See halo around organisms

Question 34

What pathology findings are associated with Candidiasis?

Answer 34

White plaques on tongue, buccal cavity, esophagus, intestines

Question 35

Which systemic mycoses is associated with bat feces?

Answer 35

HIstoplasmosis

Question 36

Where can macacine herpesvirus 1 (herpes B) found during latent infection?

Answer 36

Trigeminal and lumbosacral ganglia

Question 37

Inclusion bodies seen with macacine herpesvirus I infection?

Answer 37

Intranuclear inclusion bodies (Cowdry type A)

Question 38

What clinical signs does papiine herpesvirus 2 cause in baboons?

Answer 38

Often asymptomatic +/- transient oral and genital lesions Hepatocellular necrosis and pulmonary alveolitis in neonates

Question 39

Baboons should be kept separate from other NHPs due to what virus?

Answer 39

Papiine herpesvirus 2

Question 40

Why should saimiri be separated from aotus and callitrichids?

Answer 40

Saimirine herpesvirus 1 (SAHV1) Asymptomatic in saimiri, but fatal in aotus and callitrichids

Question 41

Clinical signs in aotus and callitrichids infected with human herpesvirus 1 or 2?

Answer 41

Multisystemic necrosis + encephalitis

Question 42

Clinical presentation of cercopithecine herpesvirus 9

Answer 42

In aberrant hosts (African and Asian NHP spp) --> Vesicular dermatitis (rarely involving hands/feet) progressing to death within 48 hours

Question 43

Clinical presentation of SIV positive macaques infected with macacine herpesvirus 4?

Answer 43

B cell lymphoma and oral hairy leukoplakia

Question 44

What other virus are NHPs also infected with when they develop RFHV induced retroperitoneal fibromatosis?

Answer 44

Simian retrovirus 2 (SRV2)

Question 45

Clinical presentation of saimiriine herpesvirus 2 and ateline herpesvirus 2,3?

Answer 45

None in natural host (Squirrel and spider monkeys) Lymphoma in callitrichids and aotus